Occurrence and molecular characterization of cephalosporin resistant escherichia coli isolates from chicken meat
Contamination of retail meat with extended spectrum beta-lactamase (ESBL) and/or AmpC type beta-lactamase (AmpC) producing Escherichia coli may contribute to increased incidences of infections in humans. Regular monitoring of these bacteria is required in the view of one health approach. In this stu...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2020-05-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2710 |
| _version_ | 1797800709163319296 |
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| author | Nebahat BİLGE Çiğdem SEZER Leyla VATANSEVER Sevda PEHLİVANLAR ÖNEN |
| author_facet | Nebahat BİLGE Çiğdem SEZER Leyla VATANSEVER Sevda PEHLİVANLAR ÖNEN |
| author_sort | Nebahat BİLGE |
| collection | DOAJ |
| description | Contamination of retail meat with extended spectrum beta-lactamase (ESBL) and/or AmpC type beta-lactamase (AmpC) producing
Escherichia coli may contribute to increased incidences of infections in humans. Regular monitoring of these bacteria is required in the view
of one health approach. In this study, 100 chicken meat samples obtained from Kars, Turkey were analysed and 214 isolates recovered from
72/100 samples were identified as E. coli. Antibiotic susceptibility tests showed that 15.42% of isolates (33/214) were resistant to at least one
cephalosporin antibiotic, 20 (9.34%) were beta-lactamase producer. Among beta-lactamase producing E. coli isolates 8 had blaTEM, 7 had
blaCTX-M and 3 had blaSHV genes. Plasmid-mediated AmpC beta-lactamase (blaCMY) gene was present in 13 isolates. Plasmid mediated quinolone
resistance genes were also screened by polymerase chain reaction and identified by sequencing of the isolates. As a result, 3 isolates were
found to be positive for qnrB, whereas the qnrS gene was detected in 4 isolates. Regarding the virulence genes 19 isolates were positive for
fimH and 2 isolates were carrying kpsMT II. Phylo-group D2, A0, A1, B1 and D1 were detected in 13, 2, 2, 2 and 1 isolates, respectively. Our
findings indicate that poultry meat could be an important carrier of ESBL positive E. coli. |
| first_indexed | 2024-03-13T04:39:22Z |
| format | Article |
| id | doaj.art-7124c487ecd94a23a621c3121e0cc669 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:22Z |
| publishDate | 2020-05-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-7124c487ecd94a23a621c3121e0cc6692023-06-19T06:32:43ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512020-05-0126446346810.9775/kvfd.2019.235142710Occurrence and molecular characterization of cephalosporin resistant escherichia coli isolates from chicken meatNebahat BİLGE0Çiğdem SEZER1Leyla VATANSEVER2Sevda PEHLİVANLAR ÖNENUniversity of Kafkas, Faculty of Veterinary Medicine, Department of Food Safety and Public Health, TR-36100 Kars - TURKEYUniversity of Kafkas, Faculty of Veterinary Medicine, Department of Food Safety and Public Health, TR-36100 Kars - TURKEYUniversity of Kafkas, Faculty of Veterinary Medicine, Department of Food Safety and Public Health, TR-36100 Kars - TURKEYContamination of retail meat with extended spectrum beta-lactamase (ESBL) and/or AmpC type beta-lactamase (AmpC) producing Escherichia coli may contribute to increased incidences of infections in humans. Regular monitoring of these bacteria is required in the view of one health approach. In this study, 100 chicken meat samples obtained from Kars, Turkey were analysed and 214 isolates recovered from 72/100 samples were identified as E. coli. Antibiotic susceptibility tests showed that 15.42% of isolates (33/214) were resistant to at least one cephalosporin antibiotic, 20 (9.34%) were beta-lactamase producer. Among beta-lactamase producing E. coli isolates 8 had blaTEM, 7 had blaCTX-M and 3 had blaSHV genes. Plasmid-mediated AmpC beta-lactamase (blaCMY) gene was present in 13 isolates. Plasmid mediated quinolone resistance genes were also screened by polymerase chain reaction and identified by sequencing of the isolates. As a result, 3 isolates were found to be positive for qnrB, whereas the qnrS gene was detected in 4 isolates. Regarding the virulence genes 19 isolates were positive for fimH and 2 isolates were carrying kpsMT II. Phylo-group D2, A0, A1, B1 and D1 were detected in 13, 2, 2, 2 and 1 isolates, respectively. Our findings indicate that poultry meat could be an important carrier of ESBL positive E. coli.https://vetdergikafkas.org/pdf.php?id=2710escherichia colichickencephalosporinesblampc |
| spellingShingle | Nebahat BİLGE Çiğdem SEZER Leyla VATANSEVER Sevda PEHLİVANLAR ÖNEN Occurrence and molecular characterization of cephalosporin resistant escherichia coli isolates from chicken meat Kafkas Universitesi Veteriner Fakültesi Dergisi escherichia coli chicken cephalosporin esbl ampc |
| title | Occurrence and molecular characterization of cephalosporin resistant escherichia coli isolates from chicken meat |
| title_full | Occurrence and molecular characterization of cephalosporin resistant escherichia coli isolates from chicken meat |
| title_fullStr | Occurrence and molecular characterization of cephalosporin resistant escherichia coli isolates from chicken meat |
| title_full_unstemmed | Occurrence and molecular characterization of cephalosporin resistant escherichia coli isolates from chicken meat |
| title_short | Occurrence and molecular characterization of cephalosporin resistant escherichia coli isolates from chicken meat |
| title_sort | occurrence and molecular characterization of cephalosporin resistant escherichia coli isolates from chicken meat |
| topic | escherichia coli chicken cephalosporin esbl ampc |
| url | https://vetdergikafkas.org/pdf.php?id=2710 |
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