Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro

Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro

Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For <i>Lycoris</i> species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of <i>Lycoris sprengeri</i>...

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Bibliographic Details
Main Authors: Ziming Ren, Yunchen Xu, Xuesi Lvy, Dong Zhang, Cong Gao, Yefan Lin, Yue Liu, Yun Wu, Yiping Xia
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:International Journal of Molecular Sciences
Subjects:
6-benzyladenine
bulblet regeneration
sucrose cleavage
endogenous hormone
cell wall invertase
<i>Lycoris</i> <i>sprengeri</i>
Online Access:https://www.mdpi.com/1422-0067/22/21/11890
Description
Summary:Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For <i>Lycoris</i> species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of <i>Lycoris sprengeri</i> (<i>Ls</i>) derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media. BA application did not change the biological processes among groups but led to earlier decreases in sucrose and total soluble sugar (TSS) contents. Correlation analyses showed that the BA treatments changed the interaction between carbohydrate and endogenous hormone contents during bulblet regeneration. We found that two sucrose degradation enzyme-related genes, cell wall invertase (CWIN) and sucrose synthase, exhibited exactly opposite expression patterns during the competence stage. In addition, the regeneration system that obtained more bulblets showed significantly higher expression of <i>LsCWIN2</i> than those that obtained fewer bulblets. Our data demonstrate the essential role of BA in accelerating sucrose degradation and the selection of a dominant sucrose cleavage pattern at the competence stage of in vitro bulblet regeneration. We propose that a relatively active CWIN-catalyzed pathway at the competence stage might promote bulblet regeneration, thus influencing bulb yield.
ISSN:1661-6596
1422-0067

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