Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro
Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For <i>Lycoris</i> species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of <i>Lycoris sprengeri</i>...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11890 |
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| author | Ziming Ren Yunchen Xu Xuesi Lvy Dong Zhang Cong Gao Yefan Lin Yue Liu Yun Wu Yiping Xia |
| author_facet | Ziming Ren Yunchen Xu Xuesi Lvy Dong Zhang Cong Gao Yefan Lin Yue Liu Yun Wu Yiping Xia |
| author_sort | Ziming Ren |
| collection | DOAJ |
| description | Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For <i>Lycoris</i> species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of <i>Lycoris sprengeri</i> (<i>Ls</i>) derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media. BA application did not change the biological processes among groups but led to earlier decreases in sucrose and total soluble sugar (TSS) contents. Correlation analyses showed that the BA treatments changed the interaction between carbohydrate and endogenous hormone contents during bulblet regeneration. We found that two sucrose degradation enzyme-related genes, cell wall invertase (CWIN) and sucrose synthase, exhibited exactly opposite expression patterns during the competence stage. In addition, the regeneration system that obtained more bulblets showed significantly higher expression of <i>LsCWIN2</i> than those that obtained fewer bulblets. Our data demonstrate the essential role of BA in accelerating sucrose degradation and the selection of a dominant sucrose cleavage pattern at the competence stage of in vitro bulblet regeneration. We propose that a relatively active CWIN-catalyzed pathway at the competence stage might promote bulblet regeneration, thus influencing bulb yield. |
| first_indexed | 2024-03-10T06:00:43Z |
| format | Article |
| id | doaj.art-7124da62c8474752b414798ccb9affe3 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:00:43Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7124da62c8474752b414798ccb9affe32023-11-22T20:59:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122211189010.3390/ijms222111890Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In VitroZiming Ren0Yunchen Xu1Xuesi Lvy2Dong Zhang3Cong Gao4Yefan Lin5Yue Liu6Yun Wu7Yiping Xia8Genomics and Genetic Engineering Laboratory of Ornamental Plants, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaGenomics and Genetic Engineering Laboratory of Ornamental Plants, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaGenomics and Genetic Engineering Laboratory of Ornamental Plants, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaGenomics and Genetic Engineering Laboratory of Ornamental Plants, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaGenomics and Genetic Engineering Laboratory of Ornamental Plants, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaGenomics and Genetic Engineering Laboratory of Ornamental Plants, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaGenomics and Genetic Engineering Laboratory of Ornamental Plants, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Landscape Architecture, School of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaGenomics and Genetic Engineering Laboratory of Ornamental Plants, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaBulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For <i>Lycoris</i> species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of <i>Lycoris sprengeri</i> (<i>Ls</i>) derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media. BA application did not change the biological processes among groups but led to earlier decreases in sucrose and total soluble sugar (TSS) contents. Correlation analyses showed that the BA treatments changed the interaction between carbohydrate and endogenous hormone contents during bulblet regeneration. We found that two sucrose degradation enzyme-related genes, cell wall invertase (CWIN) and sucrose synthase, exhibited exactly opposite expression patterns during the competence stage. In addition, the regeneration system that obtained more bulblets showed significantly higher expression of <i>LsCWIN2</i> than those that obtained fewer bulblets. Our data demonstrate the essential role of BA in accelerating sucrose degradation and the selection of a dominant sucrose cleavage pattern at the competence stage of in vitro bulblet regeneration. We propose that a relatively active CWIN-catalyzed pathway at the competence stage might promote bulblet regeneration, thus influencing bulb yield.https://www.mdpi.com/1422-0067/22/21/118906-benzyladeninebulblet regenerationsucrose cleavageendogenous hormonecell wall invertase<i>Lycoris</i> <i>sprengeri</i> |
| spellingShingle | Ziming Ren Yunchen Xu Xuesi Lvy Dong Zhang Cong Gao Yefan Lin Yue Liu Yun Wu Yiping Xia Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro International Journal of Molecular Sciences 6-benzyladenine bulblet regeneration sucrose cleavage endogenous hormone cell wall invertase <i>Lycoris</i> <i>sprengeri</i> |
| title | Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro |
| title_full | Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro |
| title_fullStr | Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro |
| title_full_unstemmed | Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro |
| title_short | Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence <i>Lycoris sprengeri</i> Bulblet Regeneration In Vitro |
| title_sort | early sucrose degradation and the dominant sucrose cleavage pattern influence i lycoris sprengeri i bulblet regeneration in vitro |
| topic | 6-benzyladenine bulblet regeneration sucrose cleavage endogenous hormone cell wall invertase <i>Lycoris</i> <i>sprengeri</i> |
| url | https://www.mdpi.com/1422-0067/22/21/11890 |
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