Transcriptome analysis of some genes of Staphylococcus aureus exposed to alkylated, nano- and native chitosans
Chitosan, including native, N- alkyl (AlkCs), and nanoparticles chitosan (CsNPs), were prepared and characterized. Average size and hydrodynamic diameter of nanoparticles were determined at 126 nm and 240 nm with a polydispersity index (PDI) of 0.154 and zeta potential of 30 mV, respectively. Crysta...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893923000725 |
| _version_ | 1827577189015486464 |
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| author | Vasighe Sadat Mirbagheri Alireza Alishahi Seyyed Hamid Reza Hashemi Petroudi Seyed Mahdi Ojagh |
| author_facet | Vasighe Sadat Mirbagheri Alireza Alishahi Seyyed Hamid Reza Hashemi Petroudi Seyed Mahdi Ojagh |
| author_sort | Vasighe Sadat Mirbagheri |
| collection | DOAJ |
| description | Chitosan, including native, N- alkyl (AlkCs), and nanoparticles chitosan (CsNPs), were prepared and characterized. Average size and hydrodynamic diameter of nanoparticles were determined at 126 nm and 240 nm with a polydispersity index (PDI) of 0.154 and zeta potential of 30 mV, respectively. Crystallinity index for Cs, AlkCs, and CsNPs was calculated as 57.85, 30.24, and 24.85, respectively. Minimum concentration of growth inhibition (125.78 µg/ml) and lethality (100 µg/ml) was observed for CsNPs (P < 0.05). CsNPs and AlkCs showed the highest diameter of the growth inhibition zone at 1250 μg/mL concentration, respectively (P < 0.05). Membrane permeability test, the highest absorption of ortho-nitrophenol was observed after 90 min with 0.11 nm. The most significant decrease in absorption in chitosan was observed at the end of 120 min. DAPI (4′,6-Diamidino-2-Phenylindole) staining test showed higher cell death of bacteria treated with AlkCs and CsNPs. Images obtained from atomic force microscopy (AFM) showed a change in the morphology of the treated cells compared to the spherical and clustered control cells. A significant increase in the expression of vraS, vraR, lytM, lrgA and lrgB genes was observed as a result of induction of S. aureus cell wall by Cs, AlkCs and CsNPs, d. |
| first_indexed | 2024-03-08T21:24:17Z |
| format | Article |
| id | doaj.art-7126a4da6f984c4396551b12a8c572cc |
| institution | Directory Open Access Journal |
| issn | 2666-8939 |
| language | English |
| last_indexed | 2024-03-08T21:24:17Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj.art-7126a4da6f984c4396551b12a8c572cc2023-12-21T07:37:40ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392023-12-016100351Transcriptome analysis of some genes of Staphylococcus aureus exposed to alkylated, nano- and native chitosansVasighe Sadat Mirbagheri0Alireza Alishahi1Seyyed Hamid Reza Hashemi Petroudi2Seyed Mahdi Ojagh3Faculty of Fisheries and Environment Science, Gorgan University of Agriculture Science and Natural Resources, Gorgan, IranFaculty of Fisheries and Environment Science, Gorgan University of Agriculture Science and Natural Resources, Gorgan, Iran; Corresponding author.Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, PO Box 578, Sari, IranFaculty of Fisheries and Environment Science, Gorgan University of Agriculture Science and Natural Resources, Gorgan, IranChitosan, including native, N- alkyl (AlkCs), and nanoparticles chitosan (CsNPs), were prepared and characterized. Average size and hydrodynamic diameter of nanoparticles were determined at 126 nm and 240 nm with a polydispersity index (PDI) of 0.154 and zeta potential of 30 mV, respectively. Crystallinity index for Cs, AlkCs, and CsNPs was calculated as 57.85, 30.24, and 24.85, respectively. Minimum concentration of growth inhibition (125.78 µg/ml) and lethality (100 µg/ml) was observed for CsNPs (P < 0.05). CsNPs and AlkCs showed the highest diameter of the growth inhibition zone at 1250 μg/mL concentration, respectively (P < 0.05). Membrane permeability test, the highest absorption of ortho-nitrophenol was observed after 90 min with 0.11 nm. The most significant decrease in absorption in chitosan was observed at the end of 120 min. DAPI (4′,6-Diamidino-2-Phenylindole) staining test showed higher cell death of bacteria treated with AlkCs and CsNPs. Images obtained from atomic force microscopy (AFM) showed a change in the morphology of the treated cells compared to the spherical and clustered control cells. A significant increase in the expression of vraS, vraR, lytM, lrgA and lrgB genes was observed as a result of induction of S. aureus cell wall by Cs, AlkCs and CsNPs, d.http://www.sciencedirect.com/science/article/pii/S2666893923000725Modified chitosanAntibacterialStaphylococcus aureusCell deathMembrane permeability |
| spellingShingle | Vasighe Sadat Mirbagheri Alireza Alishahi Seyyed Hamid Reza Hashemi Petroudi Seyed Mahdi Ojagh Transcriptome analysis of some genes of Staphylococcus aureus exposed to alkylated, nano- and native chitosans Carbohydrate Polymer Technologies and Applications Modified chitosan Antibacterial Staphylococcus aureus Cell death Membrane permeability |
| title | Transcriptome analysis of some genes of Staphylococcus aureus exposed to alkylated, nano- and native chitosans |
| title_full | Transcriptome analysis of some genes of Staphylococcus aureus exposed to alkylated, nano- and native chitosans |
| title_fullStr | Transcriptome analysis of some genes of Staphylococcus aureus exposed to alkylated, nano- and native chitosans |
| title_full_unstemmed | Transcriptome analysis of some genes of Staphylococcus aureus exposed to alkylated, nano- and native chitosans |
| title_short | Transcriptome analysis of some genes of Staphylococcus aureus exposed to alkylated, nano- and native chitosans |
| title_sort | transcriptome analysis of some genes of staphylococcus aureus exposed to alkylated nano and native chitosans |
| topic | Modified chitosan Antibacterial Staphylococcus aureus Cell death Membrane permeability |
| url | http://www.sciencedirect.com/science/article/pii/S2666893923000725 |
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