Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production
The objective of this study was to evaluate the effects of dietary Bacillus subtilis supplementation and calcium (Ca) levels on performance, eggshell quality, intestinal morphology, and relative calbindin-D28k (CALB1) mRNA level of laying hens in the late phase of production. An experiment employing...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-03-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912031021X |
| _version_ | 1818353400244338688 |
|---|---|
| author | Jing Wang Wei-wei Wang Guang-hai Qi Chuang-fei Cui Shu-geng Wu Hai-jun Zhang Li Xu Jing Wang |
| author_facet | Jing Wang Wei-wei Wang Guang-hai Qi Chuang-fei Cui Shu-geng Wu Hai-jun Zhang Li Xu Jing Wang |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | The objective of this study was to evaluate the effects of dietary Bacillus subtilis supplementation and calcium (Ca) levels on performance, eggshell quality, intestinal morphology, and relative calbindin-D28k (CALB1) mRNA level of laying hens in the late phase of production. An experiment employing a 2 × 3 factorial arrangement of 3 levels of Ca (3.5, 4.0, and 4.5%) and the absence or presence of B. subtilis was carried out with a total of 576 Hy-Line Brown laying hens aged 72 to 79 wk. Every group had 8 replicates of 12 birds each. The results showed that 4.0 and 4.5% Ca levels improved (P < 0.05) apparent retention and serum Ca content of aged laying hens. Compared with the 3.5% Ca level, the 4.0% Ca level in diets increased (P < 0.05) thickness, eggshell weight, shell ratio, and eggshell Ca content of aged laying hens. Moreover, breaking strength, thickness, eggshell weight, shell ratio, eggshell Ca content, apparent retention of Ca in g/day, apparent retention of Ca in percent, villus height, villus height/crypt depth, serum Ca level, and relative CALB1 mRNA level of aged laying hens were all increased (P < 0.05) by B. subtilis supplementation in diets. The supplemental B. subtilis decreased feed conversion ratio (P = 0.001) significantly. In addition, there was an interaction effect between increased Ca levels from 3.5 to 4.5% and B. subtilis supplementation on crypt depth in the duodenum (P < 0.05). In conclusion, we found that both the increase in dietary Ca level from 3.5 to 4.5% and B. subtilis supplementation could enhance intestinal Ca absorption and improve eggshell quality of laying hens in the late phase of production (72–79 wk of age). Dietary supplementation of B. subtilis accompanying the 4.0% Ca level was appropriate in enhancement of eggshell quality. |
| first_indexed | 2024-12-13T19:08:55Z |
| format | Article |
| id | doaj.art-7127f5fd6b4346a6bd0d754337366989 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-13T19:08:55Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-7127f5fd6b4346a6bd0d7543373669892022-12-21T23:34:27ZengElsevierPoultry Science0032-57912021-03-011003100970Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of productionJing Wang0Wei-wei Wang1Guang-hai Qi2Chuang-fei Cui3Shu-geng Wu4Hai-jun Zhang5Li Xu6Jing Wang7Laboratory of Quality & Safety Risk Assessment For Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, and National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Animal Science and Technology, Northeast Agriculture University, Harbin, Heilongjiang 150000, ChinaLaboratory of Quality & Safety Risk Assessment For Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, and National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaLaboratory of Quality & Safety Risk Assessment For Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, and National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaLaboratory of Quality & Safety Risk Assessment For Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, and National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaLaboratory of Quality & Safety Risk Assessment For Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, and National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaLaboratory of Quality & Safety Risk Assessment For Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, and National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Animal Science and Technology, Northeast Agriculture University, Harbin, Heilongjiang 150000, ChinaLaboratory of Quality & Safety Risk Assessment For Animal Products on Feed Hazards (Beijing) of the Ministry of Agriculture & Rural Affairs, and National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding author:The objective of this study was to evaluate the effects of dietary Bacillus subtilis supplementation and calcium (Ca) levels on performance, eggshell quality, intestinal morphology, and relative calbindin-D28k (CALB1) mRNA level of laying hens in the late phase of production. An experiment employing a 2 × 3 factorial arrangement of 3 levels of Ca (3.5, 4.0, and 4.5%) and the absence or presence of B. subtilis was carried out with a total of 576 Hy-Line Brown laying hens aged 72 to 79 wk. Every group had 8 replicates of 12 birds each. The results showed that 4.0 and 4.5% Ca levels improved (P < 0.05) apparent retention and serum Ca content of aged laying hens. Compared with the 3.5% Ca level, the 4.0% Ca level in diets increased (P < 0.05) thickness, eggshell weight, shell ratio, and eggshell Ca content of aged laying hens. Moreover, breaking strength, thickness, eggshell weight, shell ratio, eggshell Ca content, apparent retention of Ca in g/day, apparent retention of Ca in percent, villus height, villus height/crypt depth, serum Ca level, and relative CALB1 mRNA level of aged laying hens were all increased (P < 0.05) by B. subtilis supplementation in diets. The supplemental B. subtilis decreased feed conversion ratio (P = 0.001) significantly. In addition, there was an interaction effect between increased Ca levels from 3.5 to 4.5% and B. subtilis supplementation on crypt depth in the duodenum (P < 0.05). In conclusion, we found that both the increase in dietary Ca level from 3.5 to 4.5% and B. subtilis supplementation could enhance intestinal Ca absorption and improve eggshell quality of laying hens in the late phase of production (72–79 wk of age). Dietary supplementation of B. subtilis accompanying the 4.0% Ca level was appropriate in enhancement of eggshell quality.http://www.sciencedirect.com/science/article/pii/S003257912031021XBacillus subtiliscalcium retentioneggshell qualitylaying henlate phase of production |
| spellingShingle | Jing Wang Wei-wei Wang Guang-hai Qi Chuang-fei Cui Shu-geng Wu Hai-jun Zhang Li Xu Jing Wang Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production Poultry Science Bacillus subtilis calcium retention eggshell quality laying hen late phase of production |
| title | Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production |
| title_full | Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production |
| title_fullStr | Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production |
| title_full_unstemmed | Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production |
| title_short | Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production |
| title_sort | effects of dietary bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production |
| topic | Bacillus subtilis calcium retention eggshell quality laying hen late phase of production |
| url | http://www.sciencedirect.com/science/article/pii/S003257912031021X |
| work_keys_str_mv | AT jingwang effectsofdietarybacillussubtilissupplementationandcalciumlevelsonperformanceandeggshellqualityoflayinghensinthelatephaseofproduction AT weiweiwang effectsofdietarybacillussubtilissupplementationandcalciumlevelsonperformanceandeggshellqualityoflayinghensinthelatephaseofproduction AT guanghaiqi effectsofdietarybacillussubtilissupplementationandcalciumlevelsonperformanceandeggshellqualityoflayinghensinthelatephaseofproduction AT chuangfeicui effectsofdietarybacillussubtilissupplementationandcalciumlevelsonperformanceandeggshellqualityoflayinghensinthelatephaseofproduction AT shugengwu effectsofdietarybacillussubtilissupplementationandcalciumlevelsonperformanceandeggshellqualityoflayinghensinthelatephaseofproduction AT haijunzhang effectsofdietarybacillussubtilissupplementationandcalciumlevelsonperformanceandeggshellqualityoflayinghensinthelatephaseofproduction AT lixu effectsofdietarybacillussubtilissupplementationandcalciumlevelsonperformanceandeggshellqualityoflayinghensinthelatephaseofproduction AT jingwang effectsofdietarybacillussubtilissupplementationandcalciumlevelsonperformanceandeggshellqualityoflayinghensinthelatephaseofproduction |