Estimating Biomass and Vitality of Microalgae for Monitoring Cultures: A Roadmap for Reliable Measurements
Estimating algal biomass is a prerequisite for monitoring growth of microalgae. Especially for large-scale production sites, the measurements must be robust, reliable, fast and easy to obtain. We compare the relevant parameters, discuss potential hurdles and provide recommendations to tackle these i...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/15/2455 |
| _version_ | 1797432913658118144 |
|---|---|
| author | Michael Schagerl Rainer Siedler Eliška Konopáčová Sameh Samir Ali |
| author_facet | Michael Schagerl Rainer Siedler Eliška Konopáčová Sameh Samir Ali |
| author_sort | Michael Schagerl |
| collection | DOAJ |
| description | Estimating algal biomass is a prerequisite for monitoring growth of microalgae. Especially for large-scale production sites, the measurements must be robust, reliable, fast and easy to obtain. We compare the relevant parameters, discuss potential hurdles and provide recommendations to tackle these issues. The focus is on optical density and in vivo autofluorescence of chlorophyll, which have proven to be ideal candidates for monitoring purposes. Beyond biomass, cell vitality is also crucial for maintaining cultures. While maximizing biomass yield is often the primary consideration, some applications require adverse growth conditions for the synthesis of high-quality compounds. The non-invasive technique of pulse-amplified modulated (PAM) fluorescence measurements provides an ideal tool and is increasingly being employed due to ever more affordable devices. We compared three devices and studied the robustness of the dark fluorescence yield of photosystem II (Fv/Fm) at various cell densities. Although the so-called inner filter effects influence the fluorescence signal, the resulting Fv/Fm remain stable and robust over a wide range of cell densities due to mutual effects. |
| first_indexed | 2024-03-09T10:09:29Z |
| format | Article |
| id | doaj.art-7129f60b85aa4ef6a7e7b63336f8af33 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T10:09:29Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-7129f60b85aa4ef6a7e7b63336f8af332023-12-01T22:53:23ZengMDPI AGCells2073-44092022-08-011115245510.3390/cells11152455Estimating Biomass and Vitality of Microalgae for Monitoring Cultures: A Roadmap for Reliable MeasurementsMichael Schagerl0Rainer Siedler1Eliška Konopáčová2Sameh Samir Ali3Department of Functional and Evolutionary Ecology, University of Vienna, Djerassiplatz 1, A-1030 Vienna, AustriaDepartment of Functional and Evolutionary Ecology, University of Vienna, Djerassiplatz 1, A-1030 Vienna, AustriaInstitute of Environmental and Chemical Engineering, University of Pardubice, Studentská 573, 530 12 Pardubice, Czech RepublicBiofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, ChinaEstimating algal biomass is a prerequisite for monitoring growth of microalgae. Especially for large-scale production sites, the measurements must be robust, reliable, fast and easy to obtain. We compare the relevant parameters, discuss potential hurdles and provide recommendations to tackle these issues. The focus is on optical density and in vivo autofluorescence of chlorophyll, which have proven to be ideal candidates for monitoring purposes. Beyond biomass, cell vitality is also crucial for maintaining cultures. While maximizing biomass yield is often the primary consideration, some applications require adverse growth conditions for the synthesis of high-quality compounds. The non-invasive technique of pulse-amplified modulated (PAM) fluorescence measurements provides an ideal tool and is increasingly being employed due to ever more affordable devices. We compared three devices and studied the robustness of the dark fluorescence yield of photosystem II (Fv/Fm) at various cell densities. Although the so-called inner filter effects influence the fluorescence signal, the resulting Fv/Fm remain stable and robust over a wide range of cell densities due to mutual effects.https://www.mdpi.com/2073-4409/11/15/2455algaegrowthbiotechnologycultivationbiomassfluorescence |
| spellingShingle | Michael Schagerl Rainer Siedler Eliška Konopáčová Sameh Samir Ali Estimating Biomass and Vitality of Microalgae for Monitoring Cultures: A Roadmap for Reliable Measurements Cells algae growth biotechnology cultivation biomass fluorescence |
| title | Estimating Biomass and Vitality of Microalgae for Monitoring Cultures: A Roadmap for Reliable Measurements |
| title_full | Estimating Biomass and Vitality of Microalgae for Monitoring Cultures: A Roadmap for Reliable Measurements |
| title_fullStr | Estimating Biomass and Vitality of Microalgae for Monitoring Cultures: A Roadmap for Reliable Measurements |
| title_full_unstemmed | Estimating Biomass and Vitality of Microalgae for Monitoring Cultures: A Roadmap for Reliable Measurements |
| title_short | Estimating Biomass and Vitality of Microalgae for Monitoring Cultures: A Roadmap for Reliable Measurements |
| title_sort | estimating biomass and vitality of microalgae for monitoring cultures a roadmap for reliable measurements |
| topic | algae growth biotechnology cultivation biomass fluorescence |
| url | https://www.mdpi.com/2073-4409/11/15/2455 |
| work_keys_str_mv | AT michaelschagerl estimatingbiomassandvitalityofmicroalgaeformonitoringculturesaroadmapforreliablemeasurements AT rainersiedler estimatingbiomassandvitalityofmicroalgaeformonitoringculturesaroadmapforreliablemeasurements AT eliskakonopacova estimatingbiomassandvitalityofmicroalgaeformonitoringculturesaroadmapforreliablemeasurements AT samehsamirali estimatingbiomassandvitalityofmicroalgaeformonitoringculturesaroadmapforreliablemeasurements |