Reliability of the saliva self-sampling with and without supervision
One of the least invasive sampling methods suitable for self-sampling is saliva spitting. The aim of this study is to evaluate the suitability of saliva self-sampling for unsupervised testing. Two self-sampling strategies were compared on the basis of visual evaluation of samples, measurement of cor...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Karolinum Press
2023-07-01
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| Series: | Acta Universitatis Carolinae: Kinanthropologica |
| Online Access: | http://www.karolinum.cz/doi/10.14712/23366052.2023.2 |
| _version_ | 1797787745023688704 |
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| author | Jana Jaklová Dytrtová Radmila Dytrtová Ishak Kovač Michal Šteffl Michal Jakl |
| author_facet | Jana Jaklová Dytrtová Radmila Dytrtová Ishak Kovač Michal Šteffl Michal Jakl |
| author_sort | Jana Jaklová Dytrtová |
| collection | DOAJ |
| description | One of the least invasive sampling methods suitable for self-sampling is saliva spitting. The aim of this study is to evaluate the suitability of saliva self-sampling for unsupervised testing. Two self-sampling strategies were compared on the basis of visual evaluation of samples, measurement of cortisol levels in samples and questionnaire survey. The saliva samples obtained by supervised self-sampling were found to be fully suitable for further analysis. In contrast, not all saliva samples obtained from unsupervised self-collection can be used: 13% non-compliance with the minimum required sample volume, 8% with some food/drink residues and 26% taken at the wrong day time. About 42% of the unsupervised probands made at least one significant error in the saliva self-collection procedure. These results indicate that the accuracy of the results based on the analysis of samples received from saliva self-sampling is limited. For clinical investigation, the presence of an inner standard (referring to the reliability of the sampling procedure) is required. |
| first_indexed | 2024-03-13T01:26:16Z |
| format | Article |
| id | doaj.art-712b39460d1c4d83b050e8fdd76a9671 |
| institution | Directory Open Access Journal |
| issn | 1212-1428 2336-6052 |
| language | English |
| last_indexed | 2024-03-13T01:26:16Z |
| publishDate | 2023-07-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Universitatis Carolinae: Kinanthropologica |
| spelling | doaj.art-712b39460d1c4d83b050e8fdd76a96712023-07-04T14:31:47ZengKarolinum PressActa Universitatis Carolinae: Kinanthropologica1212-14282336-60522023-07-01591192810.14712/23366052.2023.2Reliability of the saliva self-sampling with and without supervisionJana Jaklová Dytrtováhttps://orcid.org/0000-0002-6024-8679Radmila Dytrtováhttps://orcid.org/0000-0002-2353-801XIshak KovačMichal Štefflhttps://orcid.org/0000-0001-7297-8145Michal JaklOne of the least invasive sampling methods suitable for self-sampling is saliva spitting. The aim of this study is to evaluate the suitability of saliva self-sampling for unsupervised testing. Two self-sampling strategies were compared on the basis of visual evaluation of samples, measurement of cortisol levels in samples and questionnaire survey. The saliva samples obtained by supervised self-sampling were found to be fully suitable for further analysis. In contrast, not all saliva samples obtained from unsupervised self-collection can be used: 13% non-compliance with the minimum required sample volume, 8% with some food/drink residues and 26% taken at the wrong day time. About 42% of the unsupervised probands made at least one significant error in the saliva self-collection procedure. These results indicate that the accuracy of the results based on the analysis of samples received from saliva self-sampling is limited. For clinical investigation, the presence of an inner standard (referring to the reliability of the sampling procedure) is required.http://www.karolinum.cz/doi/10.14712/23366052.2023.2 |
| spellingShingle | Jana Jaklová Dytrtová Radmila Dytrtová Ishak Kovač Michal Šteffl Michal Jakl Reliability of the saliva self-sampling with and without supervision Acta Universitatis Carolinae: Kinanthropologica |
| title | Reliability of the saliva self-sampling with and without supervision |
| title_full | Reliability of the saliva self-sampling with and without supervision |
| title_fullStr | Reliability of the saliva self-sampling with and without supervision |
| title_full_unstemmed | Reliability of the saliva self-sampling with and without supervision |
| title_short | Reliability of the saliva self-sampling with and without supervision |
| title_sort | reliability of the saliva self sampling with and without supervision |
| url | http://www.karolinum.cz/doi/10.14712/23366052.2023.2 |
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