| Summary: | This study was designed to check the potential of secondary metabolites of the selected plants; <i>Citrullus colocynthis</i>, <i>Solanum nigrum</i>, <i>Solanum surattense,</i> <i>Calotropis procera</i>, <i>Agave americana</i>, and <i>Anagallis arvensis</i> for antioxidant, antibacterial, antifungal, and antidiabetic agents. Plant material was soaked in ethanol/methanol to get the crude extract, which was further partitioned via solvent extraction technique. GCMS and FTIR analytical techniques were applied to check the compounds responsible for causing antioxidant, antimicrobial, and antidiabetic activities. It was concluded that about 80% of studied extracts/fractions were active against α-amylase, ranging from 43 to 96%. The highest activity (96.63%) was exhibited by butanol fractions of <i>A. arvensis</i> while the least response (43.65%) was shown by the aqueous fraction of <i>C. colocynthis</i> and the methanol fraction of fruit of <i>S. surattense.</i> The highest antioxidant activity was shown by the ethyl acetate fraction of <i>Anagallis arvensis</i> (78.1%), while aqueous as well as n-hexane fractions are the least active throughout the assay. Results showed that all tested plants can be an excellent source of natural products with potential antimicrobial, antioxidant, and antidiabetic potential. The biological response of these species is depicted as a good therapeutic agent, and, in the future, it can be encapsulated for drug discovery.
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