Functions of Forkhead Box O on Glucose Metabolism in Abalone <i>Haliotis discus hannai</i> and Its Responses to High Levels of Dietary Lipid
The forkhead box O (FoxO) subfamily is a member of the forkhead transcription factor family. It has regulation functions in glucose metabolism in mammals and fish. In the present study, a gene of the <i>foxo</i> homolog in abalone <i>Haliotis discus hannai</i> was cloned. A c...
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MDPI AG
2021-02-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/2/297 |
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| author | Liu Wang Yanlin Guo Mingzhu Pan Xinxin Li Dong Huang Yue Liu Chenglong Wu Wenbing Zhang Kangsen Mai |
| author_facet | Liu Wang Yanlin Guo Mingzhu Pan Xinxin Li Dong Huang Yue Liu Chenglong Wu Wenbing Zhang Kangsen Mai |
| author_sort | Liu Wang |
| collection | DOAJ |
| description | The forkhead box O (FoxO) subfamily is a member of the forkhead transcription factor family. It has regulation functions in glucose metabolism in mammals and fish. In the present study, a gene of the <i>foxo</i> homolog in abalone <i>Haliotis discus hannai</i> was cloned. A conservative forkhead (FH) domain and a transactivation (FoxO-TAD) domain were identified. Abalone <i>foxo</i>-specific siRNA (small interfering RNA) was injected to investigate the functions of <i>foxo</i> on glucose metabolism. Knockdown of <i>foxo</i> inhibited expression of phosphoenolpyruvate carboxykinase (<i>pepck</i>) and significantly increased expressions of hexokinase (<i>hk</i>) and pyruvate kinase (<i>pk</i>), but it failed to inhibit the relative mRNA level of glucose-6-phosphatase (<i>g6pase</i>). Then, a 100-day feeding trial was conducted to investigate the response of <i>foxo</i> and glucose metabolism in abalone fed with 1.57% (LFD, low-fat diet), 3.82% (MFD, middle-fat diet) and 6.72% (HFD, high-fat diet) of dietary lipid, respectively. The insulin-signaling pathway (AKT) was depressed and FoxO was activated by the HFD, but it did not inhibit glycolysis (<i>hk</i>) or improved gluconeogenesis significantly (<i>pepck and g6pase</i>). At the same time, impaired hepatopancreas glycogen storage raised hemolymph glucose levels. In conclusion, abalone <i>foxo</i> can be regulated by dietary lipid and can regulate gluconeogenesis or glycolysis in response to changes of dietary lipid levels, in which glycogen metabolism plays an important role. |
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| last_indexed | 2024-03-09T00:40:40Z |
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| series | Genes |
| spelling | doaj.art-712c580039cf41c3bd3a47689787937a2023-12-11T17:50:03ZengMDPI AGGenes2073-44252021-02-0112229710.3390/genes12020297Functions of Forkhead Box O on Glucose Metabolism in Abalone <i>Haliotis discus hannai</i> and Its Responses to High Levels of Dietary LipidLiu Wang0Yanlin Guo1Mingzhu Pan2Xinxin Li3Dong Huang4Yue Liu5Chenglong Wu6Wenbing Zhang7Kangsen Mai8The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaThe Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaThe Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaThe Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaThe Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaThe Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaSchool of Life Science, Huzhou University, 759 East 2nd Road, Huzhou 313000, ChinaThe Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaThe Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture and Rural Affairs), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaThe forkhead box O (FoxO) subfamily is a member of the forkhead transcription factor family. It has regulation functions in glucose metabolism in mammals and fish. In the present study, a gene of the <i>foxo</i> homolog in abalone <i>Haliotis discus hannai</i> was cloned. A conservative forkhead (FH) domain and a transactivation (FoxO-TAD) domain were identified. Abalone <i>foxo</i>-specific siRNA (small interfering RNA) was injected to investigate the functions of <i>foxo</i> on glucose metabolism. Knockdown of <i>foxo</i> inhibited expression of phosphoenolpyruvate carboxykinase (<i>pepck</i>) and significantly increased expressions of hexokinase (<i>hk</i>) and pyruvate kinase (<i>pk</i>), but it failed to inhibit the relative mRNA level of glucose-6-phosphatase (<i>g6pase</i>). Then, a 100-day feeding trial was conducted to investigate the response of <i>foxo</i> and glucose metabolism in abalone fed with 1.57% (LFD, low-fat diet), 3.82% (MFD, middle-fat diet) and 6.72% (HFD, high-fat diet) of dietary lipid, respectively. The insulin-signaling pathway (AKT) was depressed and FoxO was activated by the HFD, but it did not inhibit glycolysis (<i>hk</i>) or improved gluconeogenesis significantly (<i>pepck and g6pase</i>). At the same time, impaired hepatopancreas glycogen storage raised hemolymph glucose levels. In conclusion, abalone <i>foxo</i> can be regulated by dietary lipid and can regulate gluconeogenesis or glycolysis in response to changes of dietary lipid levels, in which glycogen metabolism plays an important role.https://www.mdpi.com/2073-4425/12/2/297<i>Haliotis discus hannai</i>forkhead box Oglucose metabolismlipidinsulin sensitivity |
| spellingShingle | Liu Wang Yanlin Guo Mingzhu Pan Xinxin Li Dong Huang Yue Liu Chenglong Wu Wenbing Zhang Kangsen Mai Functions of Forkhead Box O on Glucose Metabolism in Abalone <i>Haliotis discus hannai</i> and Its Responses to High Levels of Dietary Lipid Genes <i>Haliotis discus hannai</i> forkhead box O glucose metabolism lipid insulin sensitivity |
| title | Functions of Forkhead Box O on Glucose Metabolism in Abalone <i>Haliotis discus hannai</i> and Its Responses to High Levels of Dietary Lipid |
| title_full | Functions of Forkhead Box O on Glucose Metabolism in Abalone <i>Haliotis discus hannai</i> and Its Responses to High Levels of Dietary Lipid |
| title_fullStr | Functions of Forkhead Box O on Glucose Metabolism in Abalone <i>Haliotis discus hannai</i> and Its Responses to High Levels of Dietary Lipid |
| title_full_unstemmed | Functions of Forkhead Box O on Glucose Metabolism in Abalone <i>Haliotis discus hannai</i> and Its Responses to High Levels of Dietary Lipid |
| title_short | Functions of Forkhead Box O on Glucose Metabolism in Abalone <i>Haliotis discus hannai</i> and Its Responses to High Levels of Dietary Lipid |
| title_sort | functions of forkhead box o on glucose metabolism in abalone i haliotis discus hannai i and its responses to high levels of dietary lipid |
| topic | <i>Haliotis discus hannai</i> forkhead box O glucose metabolism lipid insulin sensitivity |
| url | https://www.mdpi.com/2073-4425/12/2/297 |
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