Effects of micro-colloidal Rhodomyrtus tomentosa on MMP9, GLUT-1, and IL-1β expression in Rattus norvegicus cervical cancer
Context: Rhodomyrtus tomentosa has the potential to contain compounds that enhance health. One type of disease that requires antioxidants in its healing is cancer. R. tomentosa extract was resized to nano size to optimize cell permeability. Aims: To analyze the effects of micro-colloidal leaves e...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2023-05-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol11/jppres23.1618_11.3.537.pdf |
| _version_ | 1797782138570932224 |
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| author | Cheryl Grace Pratiwi Rumahorbo Putri Cahaya Situmorang Elizabeth Rosa Meliana Puspa Zagoto Lailatun Nisfa Uswatun Hasanah |
| author_facet | Cheryl Grace Pratiwi Rumahorbo Putri Cahaya Situmorang Elizabeth Rosa Meliana Puspa Zagoto Lailatun Nisfa Uswatun Hasanah |
| author_sort | Cheryl Grace Pratiwi Rumahorbo |
| collection | DOAJ |
| description | Context: Rhodomyrtus tomentosa has the potential to contain compounds that enhance health. One type of disease that requires antioxidants in its healing is cancer. R. tomentosa extract was resized to nano size to optimize cell permeability.
Aims: To analyze the effects of micro-colloidal leaves extract of R. tomentosa (MR) on MMP9, GLUT-1, and IL-1β expression in cervical cancer.
Methods: Wistar rats were divided into five treatments, namely untreated (C-), cervical cancer (C+), and a cervical cancer rat model that was given R. tomentosa extract (MR) 100 mg/kg (MR100), 200 mg/kg (MR200), and 400 mg/kg (MR400). After receiving the extract for 30 days, all groups were analyzed using an immunohistochemical technique to detect MMP9, GLUT-1, and IL-1β antibodies.
Results: From the statistical analysis, there was a substantial difference in the expression of MMP9, GLUT-1, and IL-1 in the C+ group compared to the C-, MR100, MR200, and MR400 groups (p<0.005; p<0.002: p<0.001 p<0.01, respectively). Unrestrained cell development, asymmetric cell shape, a sizeable nucleus-to-cytoplasm ratio, and various nucleus-form variants, and when MR was administered, the cervical histology of the tissues began to improve, similar to group C. In this group decreases the distance between the tumors, slows the growth of the carcinoma, and permits the nucleus to form appropriately in a cervical cancer cell was observed. Dosages of 200 and 400 mg/kg improved the histology of cervical tissue while decreasing MMP-9, GLUT-1, and IL-1β expression.
Conclusions: Rhodomyrtus tomentosa ethanolic extract shows antimetastatic properties and could be analyzed as an anticancer therapy in the future. |
| first_indexed | 2024-03-13T00:06:47Z |
| format | Article |
| id | doaj.art-712cb37b8bcf49db9e6ddc448869ecd9 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-03-13T00:06:47Z |
| publishDate | 2023-05-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-712cb37b8bcf49db9e6ddc448869ecd92023-07-12T23:20:09ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502023-05-0111353754610.56499/jppres23.1618_11.3.537Effects of micro-colloidal Rhodomyrtus tomentosa on MMP9, GLUT-1, and IL-1β expression in Rattus norvegicus cervical cancerCheryl Grace Pratiwi Rumahorbo0Putri Cahaya Situmorang1Elizabeth Rosa Meliana Puspa Zagoto2Lailatun Nisfa3Uswatun Hasanah4Study Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia.Study Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia.Study Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia.Study Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia.Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, Indonesia.Context: Rhodomyrtus tomentosa has the potential to contain compounds that enhance health. One type of disease that requires antioxidants in its healing is cancer. R. tomentosa extract was resized to nano size to optimize cell permeability. Aims: To analyze the effects of micro-colloidal leaves extract of R. tomentosa (MR) on MMP9, GLUT-1, and IL-1β expression in cervical cancer. Methods: Wistar rats were divided into five treatments, namely untreated (C-), cervical cancer (C+), and a cervical cancer rat model that was given R. tomentosa extract (MR) 100 mg/kg (MR100), 200 mg/kg (MR200), and 400 mg/kg (MR400). After receiving the extract for 30 days, all groups were analyzed using an immunohistochemical technique to detect MMP9, GLUT-1, and IL-1β antibodies. Results: From the statistical analysis, there was a substantial difference in the expression of MMP9, GLUT-1, and IL-1 in the C+ group compared to the C-, MR100, MR200, and MR400 groups (p<0.005; p<0.002: p<0.001 p<0.01, respectively). Unrestrained cell development, asymmetric cell shape, a sizeable nucleus-to-cytoplasm ratio, and various nucleus-form variants, and when MR was administered, the cervical histology of the tissues began to improve, similar to group C. In this group decreases the distance between the tumors, slows the growth of the carcinoma, and permits the nucleus to form appropriately in a cervical cancer cell was observed. Dosages of 200 and 400 mg/kg improved the histology of cervical tissue while decreasing MMP-9, GLUT-1, and IL-1β expression. Conclusions: Rhodomyrtus tomentosa ethanolic extract shows antimetastatic properties and could be analyzed as an anticancer therapy in the future.https://jppres.com/jppres/pdf/vol11/jppres23.1618_11.3.537.pdfglut-1il-1βmmp9myrtusplant extractsuterine cervical neoplasms |
| spellingShingle | Cheryl Grace Pratiwi Rumahorbo Putri Cahaya Situmorang Elizabeth Rosa Meliana Puspa Zagoto Lailatun Nisfa Uswatun Hasanah Effects of micro-colloidal Rhodomyrtus tomentosa on MMP9, GLUT-1, and IL-1β expression in Rattus norvegicus cervical cancer Journal of Pharmacy & Pharmacognosy Research glut-1 il-1β mmp9 myrtus plant extracts uterine cervical neoplasms |
| title | Effects of micro-colloidal Rhodomyrtus tomentosa on MMP9, GLUT-1, and IL-1β expression in Rattus norvegicus cervical cancer |
| title_full | Effects of micro-colloidal Rhodomyrtus tomentosa on MMP9, GLUT-1, and IL-1β expression in Rattus norvegicus cervical cancer |
| title_fullStr | Effects of micro-colloidal Rhodomyrtus tomentosa on MMP9, GLUT-1, and IL-1β expression in Rattus norvegicus cervical cancer |
| title_full_unstemmed | Effects of micro-colloidal Rhodomyrtus tomentosa on MMP9, GLUT-1, and IL-1β expression in Rattus norvegicus cervical cancer |
| title_short | Effects of micro-colloidal Rhodomyrtus tomentosa on MMP9, GLUT-1, and IL-1β expression in Rattus norvegicus cervical cancer |
| title_sort | effects of micro colloidal rhodomyrtus tomentosa on mmp9 glut 1 and il 1β expression in rattus norvegicus cervical cancer |
| topic | glut-1 il-1β mmp9 myrtus plant extracts uterine cervical neoplasms |
| url | https://jppres.com/jppres/pdf/vol11/jppres23.1618_11.3.537.pdf |
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