TGFB1I1 promotes cell proliferation and migration in urothelial carcinoma
Abstract Urothelial carcinoma (UC) is common cancer worldwide with a high prevalence in Taiwan, especially in the upper urinary tract, including the renal pelvis and ureter, also classifying as upper urinary tract urothelial carcinoma. Here, we aim to find a representative prognostic marker that str...
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Kaohsiung Journal of Medical Sciences |
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| Online Access: | https://doi.org/10.1002/kjm2.12798 |
| _version_ | 1797265461761540096 |
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| author | Peir‐In Liang Yu‐Ching Wei Huan‐Da Chen Yu‐Chun Ma Hung‐Lung Ke Chu‐Chun Chien Hao‐Wen Chuang |
| author_facet | Peir‐In Liang Yu‐Ching Wei Huan‐Da Chen Yu‐Chun Ma Hung‐Lung Ke Chu‐Chun Chien Hao‐Wen Chuang |
| author_sort | Peir‐In Liang |
| collection | DOAJ |
| description | Abstract Urothelial carcinoma (UC) is common cancer worldwide with a high prevalence in Taiwan, especially in the upper urinary tract, including the renal pelvis and ureter, also classifying as upper urinary tract urothelial carcinoma. Here, we aim to find a representative prognostic marker that strongly correlates to this type of carcinoma. Transforming growth factor beta‐1‐induced transcript 1 (TGFB1I1) is a cofactor of cellular TGF‐β1 and interacts with various nuclear receptors. The previous study showed that TGFB1I1 promotes focal adhesion formation, contributing to the epithelial‐mesenchymal transition (EMT) with actin cytoskeleton and vimentin through TGFB1I1 regulation. We aim to reveal the role of TGFB1I1 in the tumorigenesis of UC. In silico and clinicopathological data of upper urinary tract urothelial carcinoma (UTUC) and urinary bladder urothelial carcinoma (UBUC) were accessed and analyzed for IHC staining regarding tumor characteristics, including survival outcome. Finally, an in vitro study was performed to demonstrate the biological changes of UC cells. In UTUC, overexpression of TGFB1I1 was significantly correlated with advanced tumor stage, papillary configuration, and frequent mitosis. Meanwhile, overexpression of TGFB1I1 was significantly correlated with advanced tumor stage and histological grade in UBUC. Moreover, the in vitro study shows that TGFB1I1 affects cell proliferation, viability, migration and wound healing. The EMT markers also decreased upon TGFB1I1 knockdown. In this study, we identified that TGFB1I1 regulates UC cell proliferation and viability and induces the EMT to facilitate cell migration in vitro, leading to its essential role in promoting tumor aggressiveness in both UTUC and UBUC. |
| first_indexed | 2024-04-25T00:45:10Z |
| format | Article |
| id | doaj.art-712f5fb3deaa481bae2e8611a62ff4cf |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-04-25T00:45:10Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-712f5fb3deaa481bae2e8611a62ff4cf2024-03-12T05:57:09ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502024-03-0140326927910.1002/kjm2.12798TGFB1I1 promotes cell proliferation and migration in urothelial carcinomaPeir‐In Liang0Yu‐Ching Wei1Huan‐Da Chen2Yu‐Chun Ma3Hung‐Lung Ke4Chu‐Chun Chien5Hao‐Wen Chuang6Department of Pathology Kaohsiung Medical University Hospital Kaohsiung TaiwanDepartment of Pathology, School of Medicine, College of Medicine Kaohsiung Medical University Kaohsiung TaiwanDepartment of Pathology Kaohsiung Medical University Hospital Kaohsiung TaiwanDepartment of Pathology Kaohsiung Medical University Hospital Kaohsiung TaiwanDepartment of Urology Kaohsiung Medical University Hospital Kaohsiung TaiwanDepartment of Pathology Kaohsiung Municipal Ta‐Tung Hospital Kaohsiung TaiwanDepartment of Pathology and Laboratory Medicine Kaohsiung Veterans General Hospital Kaohsiung TaiwanAbstract Urothelial carcinoma (UC) is common cancer worldwide with a high prevalence in Taiwan, especially in the upper urinary tract, including the renal pelvis and ureter, also classifying as upper urinary tract urothelial carcinoma. Here, we aim to find a representative prognostic marker that strongly correlates to this type of carcinoma. Transforming growth factor beta‐1‐induced transcript 1 (TGFB1I1) is a cofactor of cellular TGF‐β1 and interacts with various nuclear receptors. The previous study showed that TGFB1I1 promotes focal adhesion formation, contributing to the epithelial‐mesenchymal transition (EMT) with actin cytoskeleton and vimentin through TGFB1I1 regulation. We aim to reveal the role of TGFB1I1 in the tumorigenesis of UC. In silico and clinicopathological data of upper urinary tract urothelial carcinoma (UTUC) and urinary bladder urothelial carcinoma (UBUC) were accessed and analyzed for IHC staining regarding tumor characteristics, including survival outcome. Finally, an in vitro study was performed to demonstrate the biological changes of UC cells. In UTUC, overexpression of TGFB1I1 was significantly correlated with advanced tumor stage, papillary configuration, and frequent mitosis. Meanwhile, overexpression of TGFB1I1 was significantly correlated with advanced tumor stage and histological grade in UBUC. Moreover, the in vitro study shows that TGFB1I1 affects cell proliferation, viability, migration and wound healing. The EMT markers also decreased upon TGFB1I1 knockdown. In this study, we identified that TGFB1I1 regulates UC cell proliferation and viability and induces the EMT to facilitate cell migration in vitro, leading to its essential role in promoting tumor aggressiveness in both UTUC and UBUC.https://doi.org/10.1002/kjm2.12798transforming growth factor beta‐1‐induced transcript 1upper urinary tract urothelial carcinomaurinary bladder urothelial carcinoma |
| spellingShingle | Peir‐In Liang Yu‐Ching Wei Huan‐Da Chen Yu‐Chun Ma Hung‐Lung Ke Chu‐Chun Chien Hao‐Wen Chuang TGFB1I1 promotes cell proliferation and migration in urothelial carcinoma Kaohsiung Journal of Medical Sciences transforming growth factor beta‐1‐induced transcript 1 upper urinary tract urothelial carcinoma urinary bladder urothelial carcinoma |
| title | TGFB1I1 promotes cell proliferation and migration in urothelial carcinoma |
| title_full | TGFB1I1 promotes cell proliferation and migration in urothelial carcinoma |
| title_fullStr | TGFB1I1 promotes cell proliferation and migration in urothelial carcinoma |
| title_full_unstemmed | TGFB1I1 promotes cell proliferation and migration in urothelial carcinoma |
| title_short | TGFB1I1 promotes cell proliferation and migration in urothelial carcinoma |
| title_sort | tgfb1i1 promotes cell proliferation and migration in urothelial carcinoma |
| topic | transforming growth factor beta‐1‐induced transcript 1 upper urinary tract urothelial carcinoma urinary bladder urothelial carcinoma |
| url | https://doi.org/10.1002/kjm2.12798 |
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