Vasodilator-stimulated phosphoprotein-phosphorylation by ginsenoside Ro inhibits fibrinogen binding to αIIb/β3 in thrombin-induced human platelets
Background: Glycoprotein IIb/IIIa (αIIb/β3) is involved in platelet adhesion, and triggers a series of intracellular signaling cascades, leading to platelet shape change, granule secretion, and clot retraction. In this study, we evaluated the effect of ginsenoside Ro (G-Ro) on the binding of fibrino...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845315001104 |
| _version_ | 1819136456824717312 |
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| author | Jung-Hae Shin Hyuk-Woo Kwon Hyun-Jeong Cho Man Hee Rhee Hwa-Jin Park |
| author_facet | Jung-Hae Shin Hyuk-Woo Kwon Hyun-Jeong Cho Man Hee Rhee Hwa-Jin Park |
| author_sort | Jung-Hae Shin |
| collection | DOAJ |
| description | Background: Glycoprotein IIb/IIIa (αIIb/β3) is involved in platelet adhesion, and triggers a series of intracellular signaling cascades, leading to platelet shape change, granule secretion, and clot retraction. In this study, we evaluated the effect of ginsenoside Ro (G-Ro) on the binding of fibrinogen to αIIb/β3.
Methods: We investigated the effect of G-Ro on regulation of signaling molecules affecting the binding of fibrinogen to αIIb/β3, and its final reaction, clot retraction.
Results: We found that G-Ro dose-dependently inhibited thrombin-induced platelet aggregation and attenuated the binding of fibrinogen to αIIb/β3 by phosphorylating cyclic adenosine monophosphate (cAMP)-dependently vasodilator-stimulated phosphoprotein (VASP; Ser157). In addition, G-Ro strongly abrogated the clot retraction reflecting the intensification of thrombus.
Conclusion: We demonstrate that G-Ro is a beneficial novel compound inhibiting αIIb/β3-mediated fibrinogen binding, and may prevent platelet aggregation-mediated thrombotic disease. |
| first_indexed | 2024-12-22T10:35:16Z |
| format | Article |
| id | doaj.art-7138941b1f12492c89ea9876326f42de |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-22T10:35:16Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-7138941b1f12492c89ea9876326f42de2022-12-21T18:29:13ZengElsevierJournal of Ginseng Research1226-84532016-10-0140435936510.1016/j.jgr.2015.11.003Vasodilator-stimulated phosphoprotein-phosphorylation by ginsenoside Ro inhibits fibrinogen binding to αIIb/β3 in thrombin-induced human plateletsJung-Hae Shin0Hyuk-Woo Kwon1Hyun-Jeong Cho2Man Hee Rhee3Hwa-Jin Park4Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University, Gyungnam, KoreaDepartment of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University, Gyungnam, KoreaDepartment of Biomedical Laboratory Science, College of Medical Science, Konyang University, Daejeon, KoreaLaboratory of Veterinary Physiology and Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu, KoreaDepartment of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University, Gyungnam, KoreaBackground: Glycoprotein IIb/IIIa (αIIb/β3) is involved in platelet adhesion, and triggers a series of intracellular signaling cascades, leading to platelet shape change, granule secretion, and clot retraction. In this study, we evaluated the effect of ginsenoside Ro (G-Ro) on the binding of fibrinogen to αIIb/β3. Methods: We investigated the effect of G-Ro on regulation of signaling molecules affecting the binding of fibrinogen to αIIb/β3, and its final reaction, clot retraction. Results: We found that G-Ro dose-dependently inhibited thrombin-induced platelet aggregation and attenuated the binding of fibrinogen to αIIb/β3 by phosphorylating cyclic adenosine monophosphate (cAMP)-dependently vasodilator-stimulated phosphoprotein (VASP; Ser157). In addition, G-Ro strongly abrogated the clot retraction reflecting the intensification of thrombus. Conclusion: We demonstrate that G-Ro is a beneficial novel compound inhibiting αIIb/β3-mediated fibrinogen binding, and may prevent platelet aggregation-mediated thrombotic disease.http://www.sciencedirect.com/science/article/pii/S1226845315001104cAMPclot retractionginsenoside Rofibrinogen bindingVASP (Ser157) |
| spellingShingle | Jung-Hae Shin Hyuk-Woo Kwon Hyun-Jeong Cho Man Hee Rhee Hwa-Jin Park Vasodilator-stimulated phosphoprotein-phosphorylation by ginsenoside Ro inhibits fibrinogen binding to αIIb/β3 in thrombin-induced human platelets Journal of Ginseng Research cAMP clot retraction ginsenoside Ro fibrinogen binding VASP (Ser157) |
| title | Vasodilator-stimulated phosphoprotein-phosphorylation by ginsenoside Ro inhibits fibrinogen binding to αIIb/β3 in thrombin-induced human platelets |
| title_full | Vasodilator-stimulated phosphoprotein-phosphorylation by ginsenoside Ro inhibits fibrinogen binding to αIIb/β3 in thrombin-induced human platelets |
| title_fullStr | Vasodilator-stimulated phosphoprotein-phosphorylation by ginsenoside Ro inhibits fibrinogen binding to αIIb/β3 in thrombin-induced human platelets |
| title_full_unstemmed | Vasodilator-stimulated phosphoprotein-phosphorylation by ginsenoside Ro inhibits fibrinogen binding to αIIb/β3 in thrombin-induced human platelets |
| title_short | Vasodilator-stimulated phosphoprotein-phosphorylation by ginsenoside Ro inhibits fibrinogen binding to αIIb/β3 in thrombin-induced human platelets |
| title_sort | vasodilator stimulated phosphoprotein phosphorylation by ginsenoside ro inhibits fibrinogen binding to αiib β3 in thrombin induced human platelets |
| topic | cAMP clot retraction ginsenoside Ro fibrinogen binding VASP (Ser157) |
| url | http://www.sciencedirect.com/science/article/pii/S1226845315001104 |
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