Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast Cancer
Background: Over the last few decades, tremendous progress has been achieved in the early detection and treatment of breast cancer (BC). However, the prognosis remains unsatisfactory, and the underlying processes of carcinogenesis are still unclear. The purpose of this research was to find out the r...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2023-07-01
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| Series: | Breast Cancer: Basic and Clinical Research |
| Online Access: | https://doi.org/10.1177/11782234231184378 |
| _version_ | 1797786919822688256 |
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| author | Shokufeh Razi Hossein Mozdarani Roudabeh Behzadi Andouhjerdi |
| author_facet | Shokufeh Razi Hossein Mozdarani Roudabeh Behzadi Andouhjerdi |
| author_sort | Shokufeh Razi |
| collection | DOAJ |
| description | Background: Over the last few decades, tremendous progress has been achieved in the early detection and treatment of breast cancer (BC). However, the prognosis remains unsatisfactory, and the underlying processes of carcinogenesis are still unclear. The purpose of this research was to find out the relationship between myocardial infarction–associated transcript ( MIAT ), FOXO3a , and miRNA29a-3p and evaluated the expression levels in patients compare with control and their potential as a noninvasive bioindicator in whole blood in BC. Methods: Whole blood and BC tissue are taken from patients before radiotherapy and chemotherapy. Total RNA was extracted from BC tissue and whole blood to synthesize complementary DNA (cDNA). The expression of MIAT, FOXO3a , and miRNA29a - 3p was analyzed by the quantitative reverse transcription-polymerase chain reaction (RT-qPCR) method and the sensitivity and specificity of them were determined by the receiver operating characteristic (ROC) curve. Bioinformatics analysis was used to understand the connections between MIAT, FOXO3a , and miRNA29a - 3p in human BC to develop a ceRNA (competitive endogenous RNA) network. Results: We identified that in ductal carcinoma BC tissue and whole blood, MIAT and FOXO3a were more highly expressed, whereas miRNA29a - 3p was lower compared with those in nontumor samples. There was a positive correlation between the expression levels of MIAT, FOXO3a , and miRNA29a - 3p in BC tissues and whole blood. Our results also proposed miRNA29a - 3p as a common target between MIAT and FOXO3a , and we showed them as a ceRNA network. Conclusions: This is the first study that indicates MIAT, FOXO3a , and miRNA29a - 3p as a ceRNA network, and their expression was analyzed in both BC tissue and whole blood. As a preliminary assessment, our findings indicate that combined levels of MIAT, FOXO3a , and miR29a - 3p may be considered as potential diagnostic bioindicator for BC. |
| first_indexed | 2024-03-13T01:14:37Z |
| format | Article |
| id | doaj.art-713cba0cff0a4766b5c3b15a334971b2 |
| institution | Directory Open Access Journal |
| issn | 1178-2234 |
| language | English |
| last_indexed | 2024-03-13T01:14:37Z |
| publishDate | 2023-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Breast Cancer: Basic and Clinical Research |
| spelling | doaj.art-713cba0cff0a4766b5c3b15a334971b22023-07-05T11:33:34ZengSAGE PublishingBreast Cancer: Basic and Clinical Research1178-22342023-07-011710.1177/11782234231184378Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast CancerShokufeh Razi0Hossein Mozdarani1Roudabeh Behzadi Andouhjerdi2Department of Genetics, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, IranDepartment of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Genetics, Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Tehran, IranBackground: Over the last few decades, tremendous progress has been achieved in the early detection and treatment of breast cancer (BC). However, the prognosis remains unsatisfactory, and the underlying processes of carcinogenesis are still unclear. The purpose of this research was to find out the relationship between myocardial infarction–associated transcript ( MIAT ), FOXO3a , and miRNA29a-3p and evaluated the expression levels in patients compare with control and their potential as a noninvasive bioindicator in whole blood in BC. Methods: Whole blood and BC tissue are taken from patients before radiotherapy and chemotherapy. Total RNA was extracted from BC tissue and whole blood to synthesize complementary DNA (cDNA). The expression of MIAT, FOXO3a , and miRNA29a - 3p was analyzed by the quantitative reverse transcription-polymerase chain reaction (RT-qPCR) method and the sensitivity and specificity of them were determined by the receiver operating characteristic (ROC) curve. Bioinformatics analysis was used to understand the connections between MIAT, FOXO3a , and miRNA29a - 3p in human BC to develop a ceRNA (competitive endogenous RNA) network. Results: We identified that in ductal carcinoma BC tissue and whole blood, MIAT and FOXO3a were more highly expressed, whereas miRNA29a - 3p was lower compared with those in nontumor samples. There was a positive correlation between the expression levels of MIAT, FOXO3a , and miRNA29a - 3p in BC tissues and whole blood. Our results also proposed miRNA29a - 3p as a common target between MIAT and FOXO3a , and we showed them as a ceRNA network. Conclusions: This is the first study that indicates MIAT, FOXO3a , and miRNA29a - 3p as a ceRNA network, and their expression was analyzed in both BC tissue and whole blood. As a preliminary assessment, our findings indicate that combined levels of MIAT, FOXO3a , and miR29a - 3p may be considered as potential diagnostic bioindicator for BC.https://doi.org/10.1177/11782234231184378 |
| spellingShingle | Shokufeh Razi Hossein Mozdarani Roudabeh Behzadi Andouhjerdi Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast Cancer Breast Cancer: Basic and Clinical Research |
| title | Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast Cancer |
| title_full | Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast Cancer |
| title_fullStr | Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast Cancer |
| title_full_unstemmed | Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast Cancer |
| title_short | Evaluation of the Potential Diagnostic Role of the Lnc-MIAT, miR-29a-3p, and FOXO3a ceRNA Networks as Noninvasive Circulatory Bioindicator in Ductal Carcinoma Breast Cancer |
| title_sort | evaluation of the potential diagnostic role of the lnc miat mir 29a 3p and foxo3a cerna networks as noninvasive circulatory bioindicator in ductal carcinoma breast cancer |
| url | https://doi.org/10.1177/11782234231184378 |
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