Antioxidant Capacity and Antigenotoxic Effect of <i>Hibiscus sabdariffa</i> L. Extracts Obtained with Ultrasound-Assisted Extraction Process
<i>Hibiscus sabdariffa</i> (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Ro...
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MDPI AG
2020-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/2/560 |
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| author | Gregorio Iván Peredo Pozos Mario Alberto Ruiz-López Juan Francisco Zamora Nátera Carlos Álvarez Moya Lucia Barrientos Ramírez Mónica Reynoso Silva Ramón Rodríguez Macías Pedro Macedonio García-López Ricardo González Cruz Eduardo Salcedo Pérez J. Jesús Vargas Radillo |
| author_facet | Gregorio Iván Peredo Pozos Mario Alberto Ruiz-López Juan Francisco Zamora Nátera Carlos Álvarez Moya Lucia Barrientos Ramírez Mónica Reynoso Silva Ramón Rodríguez Macías Pedro Macedonio García-López Ricardo González Cruz Eduardo Salcedo Pérez J. Jesús Vargas Radillo |
| author_sort | Gregorio Iván Peredo Pozos |
| collection | DOAJ |
| description | <i>Hibiscus sabdariffa</i> (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes. |
| first_indexed | 2024-12-11T16:10:08Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-11T16:10:08Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-714b94a6fe034310ac00253fc37b9fbc2022-12-22T00:59:05ZengMDPI AGApplied Sciences2076-34172020-01-0110256010.3390/app10020560app10020560Antioxidant Capacity and Antigenotoxic Effect of <i>Hibiscus sabdariffa</i> L. Extracts Obtained with Ultrasound-Assisted Extraction ProcessGregorio Iván Peredo Pozos0Mario Alberto Ruiz-López1Juan Francisco Zamora Nátera2Carlos Álvarez Moya3Lucia Barrientos Ramírez4Mónica Reynoso Silva5Ramón Rodríguez Macías6Pedro Macedonio García-López7Ricardo González Cruz8Eduardo Salcedo Pérez9J. Jesús Vargas Radillo10Doctorate in Sciences in Biosystematics, Ecology and Management of Natural and Agricultural Resources (Bemarena), University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Botany and Zoology, University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Botany and Zoology, University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Molecular and Cellular Biology, University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Wood, Pulp and Paper, Cucei, University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Molecular and Cellular Biology, University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Botany and Zoology, University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Botany and Zoology, University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Wood, Pulp and Paper, Cucei, University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Botany and Zoology, University of Guadalajara, Nextipac, Zapopan 45510, MexicoDepartment of Wood, Pulp and Paper, Cucei, University of Guadalajara, Nextipac, Zapopan 45510, Mexico<i>Hibiscus sabdariffa</i> (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes.https://www.mdpi.com/2076-3417/10/2/560phenolsflavonoidsantioxidantcomet assayantigenotoxic activity |
| spellingShingle | Gregorio Iván Peredo Pozos Mario Alberto Ruiz-López Juan Francisco Zamora Nátera Carlos Álvarez Moya Lucia Barrientos Ramírez Mónica Reynoso Silva Ramón Rodríguez Macías Pedro Macedonio García-López Ricardo González Cruz Eduardo Salcedo Pérez J. Jesús Vargas Radillo Antioxidant Capacity and Antigenotoxic Effect of <i>Hibiscus sabdariffa</i> L. Extracts Obtained with Ultrasound-Assisted Extraction Process Applied Sciences phenols flavonoids antioxidant comet assay antigenotoxic activity |
| title | Antioxidant Capacity and Antigenotoxic Effect of <i>Hibiscus sabdariffa</i> L. Extracts Obtained with Ultrasound-Assisted Extraction Process |
| title_full | Antioxidant Capacity and Antigenotoxic Effect of <i>Hibiscus sabdariffa</i> L. Extracts Obtained with Ultrasound-Assisted Extraction Process |
| title_fullStr | Antioxidant Capacity and Antigenotoxic Effect of <i>Hibiscus sabdariffa</i> L. Extracts Obtained with Ultrasound-Assisted Extraction Process |
| title_full_unstemmed | Antioxidant Capacity and Antigenotoxic Effect of <i>Hibiscus sabdariffa</i> L. Extracts Obtained with Ultrasound-Assisted Extraction Process |
| title_short | Antioxidant Capacity and Antigenotoxic Effect of <i>Hibiscus sabdariffa</i> L. Extracts Obtained with Ultrasound-Assisted Extraction Process |
| title_sort | antioxidant capacity and antigenotoxic effect of i hibiscus sabdariffa i l extracts obtained with ultrasound assisted extraction process |
| topic | phenols flavonoids antioxidant comet assay antigenotoxic activity |
| url | https://www.mdpi.com/2076-3417/10/2/560 |
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