Chromosomal aberrations detected by comparative genomic hybridization technique (CGH) in invasive ductal carcinoma of breast
Background: Nonlethal genetic damage is the basis for carcinogenesis. As various gene aberrations accumulate, malignant tumors are formed, regardless of whether the genetic damage is subtle or large enough to be distinguished in a karyotype. The study of chromosomal changes in tumor cells is importa...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2007-10-01
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| Series: | Tehran University Medical Journal |
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| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4216.pdf&manuscript_id=4216 |
| _version_ | 1819277642194485248 |
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| author | Nooshiravanpour P Tirgari F Ghaffari S R Abdirad A |
| author_facet | Nooshiravanpour P Tirgari F Ghaffari S R Abdirad A |
| author_sort | Nooshiravanpour P |
| collection | DOAJ |
| description | Background: Nonlethal genetic damage is the basis for carcinogenesis. As various gene aberrations accumulate, malignant tumors are formed, regardless of whether the genetic damage is subtle or large enough to be distinguished in a karyotype. The study of chromosomal changes in tumor cells is important in the identification of oncogenes and tumor suppressor genes by molecular cloning of genes in the vicinity of chromosomal aberrations. Furthermore, some specific aberrations can be of great diagnostic and prognostic value. Comparative genomic hybridization (CGH) is used to screen the entire genome for the detection and/or location chromosomal copy number changes.Methods: In this study, frozen sections of 20 primary breast tumors diagnosed as invasive ductal carcinoma from the Cancer Institute of Imam Khomeini Hospital, Tehran, Iran, were studied by CGH to detect chromosomal aberrations. We compared histopathological and immunohistochemical findings.Results: Hybridization in four of the cases was not optimal for CGH analysis and they were excluded from the study. DNA copy number changes were detected in 12 (75%) of the remaining 16 cases. Twenty-one instances of chromosomal aberrations were detected in total, including: +1q, +17q, +8q, +20q, -13q, -11q, -22q, -1p, -16q, -8p. The most frequent were +1q, +17q, +8q, -13q, similar to other studies. In three cases, we detected -13q, which is associated with axillary lymph node metastasis and was reported in one previous study. The mean numbers of chromosomal aberrations per tumor in metastatic and nonmetastatic tumors was 1.5 and 1, respectively. No other association between detected chromosomal aberrations and histopathological and immunohistochemical findings were seen.Conclusion: Since intermediately to widely invasive carcinomas are more likely to have chromosomal aberrations, CGH can be a valuable prognostic tool. Furthermore, CGH can be used to detect targeting molecules within novel amplifications which holds the potential for a new therapeutic approach for intractable cancer."n  |
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| format | Article |
| id | doaj.art-714bda93770b4805b660af69503da445 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-23T23:59:21Z |
| publishDate | 2007-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-714bda93770b4805b660af69503da4452022-12-21T17:25:10ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222007-10-01659712Chromosomal aberrations detected by comparative genomic hybridization technique (CGH) in invasive ductal carcinoma of breastNooshiravanpour PTirgari FGhaffari S RAbdirad ABackground: Nonlethal genetic damage is the basis for carcinogenesis. As various gene aberrations accumulate, malignant tumors are formed, regardless of whether the genetic damage is subtle or large enough to be distinguished in a karyotype. The study of chromosomal changes in tumor cells is important in the identification of oncogenes and tumor suppressor genes by molecular cloning of genes in the vicinity of chromosomal aberrations. Furthermore, some specific aberrations can be of great diagnostic and prognostic value. Comparative genomic hybridization (CGH) is used to screen the entire genome for the detection and/or location chromosomal copy number changes.Methods: In this study, frozen sections of 20 primary breast tumors diagnosed as invasive ductal carcinoma from the Cancer Institute of Imam Khomeini Hospital, Tehran, Iran, were studied by CGH to detect chromosomal aberrations. We compared histopathological and immunohistochemical findings.Results: Hybridization in four of the cases was not optimal for CGH analysis and they were excluded from the study. DNA copy number changes were detected in 12 (75%) of the remaining 16 cases. Twenty-one instances of chromosomal aberrations were detected in total, including: +1q, +17q, +8q, +20q, -13q, -11q, -22q, -1p, -16q, -8p. The most frequent were +1q, +17q, +8q, -13q, similar to other studies. In three cases, we detected -13q, which is associated with axillary lymph node metastasis and was reported in one previous study. The mean numbers of chromosomal aberrations per tumor in metastatic and nonmetastatic tumors was 1.5 and 1, respectively. No other association between detected chromosomal aberrations and histopathological and immunohistochemical findings were seen.Conclusion: Since intermediately to widely invasive carcinomas are more likely to have chromosomal aberrations, CGH can be a valuable prognostic tool. Furthermore, CGH can be used to detect targeting molecules within novel amplifications which holds the potential for a new therapeutic approach for intractable cancer."n http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4216.pdf&manuscript_id=4216comparative genomic hybridization (CGH)comparative genomic hybridization technique |
| spellingShingle | Nooshiravanpour P Tirgari F Ghaffari S R Abdirad A Chromosomal aberrations detected by comparative genomic hybridization technique (CGH) in invasive ductal carcinoma of breast Tehran University Medical Journal comparative genomic hybridization (CGH) comparative genomic hybridization technique |
| title | Chromosomal aberrations detected by comparative genomic hybridization technique (CGH) in invasive ductal carcinoma of breast |
| title_full | Chromosomal aberrations detected by comparative genomic hybridization technique (CGH) in invasive ductal carcinoma of breast |
| title_fullStr | Chromosomal aberrations detected by comparative genomic hybridization technique (CGH) in invasive ductal carcinoma of breast |
| title_full_unstemmed | Chromosomal aberrations detected by comparative genomic hybridization technique (CGH) in invasive ductal carcinoma of breast |
| title_short | Chromosomal aberrations detected by comparative genomic hybridization technique (CGH) in invasive ductal carcinoma of breast |
| title_sort | chromosomal aberrations detected by comparative genomic hybridization technique cgh in invasive ductal carcinoma of breast |
| topic | comparative genomic hybridization (CGH) comparative genomic hybridization technique |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4216.pdf&manuscript_id=4216 |
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