Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk
The assessment of detection sensitivity of five microbial inhibition tests (MITs), STAR (screening test for antibiotic residues) with the test strain Bacillus subtilis BGA, Delvotest® S P-NT, Total Antibiotics, Kalidos TB, and Kalidos MP with the test strain Bacillus stearothermophilus var. calidola...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2012-08-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201204-0004_comparison-of-detection-sensitivity-of-five-microbial-inhibition-tests-for-the-screening-of-aminoglycoside-resi.php |
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| author | Zuzana Sýkorová Goffová Ivona Kožárová Dionýz Máté Slavomír Marcinčák Zuzana Gondová Drahomíra Sopková |
| author_facet | Zuzana Sýkorová Goffová Ivona Kožárová Dionýz Máté Slavomír Marcinčák Zuzana Gondová Drahomíra Sopková |
| author_sort | Zuzana Sýkorová Goffová |
| collection | DOAJ |
| description | The assessment of detection sensitivity of five microbial inhibition tests (MITs), STAR (screening test for antibiotic residues) with the test strain Bacillus subtilis BGA, Delvotest® S P-NT, Total Antibiotics, Kalidos TB, and Kalidos MP with the test strain Bacillus stearothermophilus var. calidolactis to five aminoglycosides (AMGs), gentamicin, neomycin, streptomycin, kanamycin, and spectinomycin in fortified milk samples were studied. The sensitivity of MITs to AMGs was evaluated on the basis of experimental determination of detection limits (LODs) of MITs for AMGs. The LODs of these tests were compared with the maximum residue limits (MRLs) established for milk by the Commission Regulation (EU) No. 37/2010. LODs of STAR for AMGs in fortified milk samples were at the levels of MRL for neomycin (1.50 µg/g), gentamicin (0.10 µg/g), streptomycin (0.20 µg/g) and kanamycin (0.15 µg/g). Spectinomycin (0.20 µg/g) was not detected at the level of MRL. The LODs determined by Delvotest® SP-NT, Total Antibiotics and Kalidos MP were comparable, but only gentamicin and neomycin were reliably detected at the levels of MRL. Kalidos TB was more sensitive to AMGs than Delvotest® SP-NT, Total Antibiotics and Kalidos MP. Gentamicin, neomycin and streptomycin were detected at the levels of MRL. |
| first_indexed | 2024-04-10T08:33:45Z |
| format | Article |
| id | doaj.art-7150979737394811bde8030315e28ec8 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:33:45Z |
| publishDate | 2012-08-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-7150979737394811bde8030315e28ec82023-02-23T03:27:52ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172012-08-0130431432010.17221/86/2011-CJFScjf-201204-0004Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milkZuzana Sýkorová Goffová0Ivona Kožárová1Dionýz Máté2Slavomír Marcinčák3Zuzana Gondová4Drahomíra Sopková5Department of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak RepublicDepartment of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak RepublicDepartment of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak RepublicDepartment of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak RepublicDepartment of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak RepublicDepartment of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovak RepublicThe assessment of detection sensitivity of five microbial inhibition tests (MITs), STAR (screening test for antibiotic residues) with the test strain Bacillus subtilis BGA, Delvotest® S P-NT, Total Antibiotics, Kalidos TB, and Kalidos MP with the test strain Bacillus stearothermophilus var. calidolactis to five aminoglycosides (AMGs), gentamicin, neomycin, streptomycin, kanamycin, and spectinomycin in fortified milk samples were studied. The sensitivity of MITs to AMGs was evaluated on the basis of experimental determination of detection limits (LODs) of MITs for AMGs. The LODs of these tests were compared with the maximum residue limits (MRLs) established for milk by the Commission Regulation (EU) No. 37/2010. LODs of STAR for AMGs in fortified milk samples were at the levels of MRL for neomycin (1.50 µg/g), gentamicin (0.10 µg/g), streptomycin (0.20 µg/g) and kanamycin (0.15 µg/g). Spectinomycin (0.20 µg/g) was not detected at the level of MRL. The LODs determined by Delvotest® SP-NT, Total Antibiotics and Kalidos MP were comparable, but only gentamicin and neomycin were reliably detected at the levels of MRL. Kalidos TB was more sensitive to AMGs than Delvotest® SP-NT, Total Antibiotics and Kalidos MP. Gentamicin, neomycin and streptomycin were detected at the levels of MRL.https://cjfs.agriculturejournals.cz/artkey/cjf-201204-0004_comparison-of-detection-sensitivity-of-five-microbial-inhibition-tests-for-the-screening-of-aminoglycoside-resi.phpantibiotic residuesmicrobial screeningdetection limits |
| spellingShingle | Zuzana Sýkorová Goffová Ivona Kožárová Dionýz Máté Slavomír Marcinčák Zuzana Gondová Drahomíra Sopková Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk Czech Journal of Food Sciences antibiotic residues microbial screening detection limits |
| title | Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk |
| title_full | Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk |
| title_fullStr | Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk |
| title_full_unstemmed | Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk |
| title_short | Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk |
| title_sort | comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk |
| topic | antibiotic residues microbial screening detection limits |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201204-0004_comparison-of-detection-sensitivity-of-five-microbial-inhibition-tests-for-the-screening-of-aminoglycoside-resi.php |
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