Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01141/full |
| _version_ | 1818384734707777536 |
|---|---|
| author | Laurent Dortet Laurent Dortet Laurent Dortet Laurent Dortet Rémy A. Bonnin Rémy A. Bonnin Rémy A. Bonnin Simon Le Hello Laetitia Fabre Richard Bonnet Richard Bonnet Markus Kostrzewa Alain Filloux Gerald Larrouy-Maumus |
| author_facet | Laurent Dortet Laurent Dortet Laurent Dortet Laurent Dortet Rémy A. Bonnin Rémy A. Bonnin Rémy A. Bonnin Simon Le Hello Laetitia Fabre Richard Bonnet Richard Bonnet Markus Kostrzewa Alain Filloux Gerald Larrouy-Maumus |
| author_sort | Laurent Dortet |
| collection | DOAJ |
| description | Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin resistance. Currently, minimal inhibitory concentration (MIC) determination by broth microdilution, the gold standard for the detection of polymyxin resistance, is time consuming (24 h) and challenging to perform in clinical and veterinary laboratories. Here we present the use of the MALDIxin to detect colistin resistant Salmonella enterica using the MALDxin test on the routine matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system. |
| first_indexed | 2024-12-14T03:26:58Z |
| format | Article |
| id | doaj.art-7154d72b6cf24d29a529a1ae09db57da |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T03:26:58Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7154d72b6cf24d29a529a1ae09db57da2022-12-21T23:18:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01141519570Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass SpectrometerLaurent Dortet0Laurent Dortet1Laurent Dortet2Laurent Dortet3Rémy A. Bonnin4Rémy A. Bonnin5Rémy A. Bonnin6Simon Le Hello7Laetitia Fabre8Richard Bonnet9Richard Bonnet10Markus Kostrzewa11Alain Filloux12Gerald Larrouy-Maumus13MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United KingdomDepartment of Bacteriology-Hygiene, Bicêtre Hospital, Assistance Publique – Hôpitaux de Paris, Le Kremlin-Bicêtre, FranceEA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum ß-Lactamases”, Paris-Sud University, LabEx Lermit, Faculty of Medecine, Le Kremlin-Bicêtre, FranceFrench National Reference Centre for Antibiotic Resistance, Le Kremlin-Bicêtre, FranceDepartment of Bacteriology-Hygiene, Bicêtre Hospital, Assistance Publique – Hôpitaux de Paris, Le Kremlin-Bicêtre, FranceEA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum ß-Lactamases”, Paris-Sud University, LabEx Lermit, Faculty of Medecine, Le Kremlin-Bicêtre, FranceFrench National Reference Centre for Antibiotic Resistance, Le Kremlin-Bicêtre, FranceInstitut Pasteur, French National Reference Centre for E. coli, Shigella and Salmonella, Paris, FranceInstitut Pasteur, French National Reference Centre for E. coli, Shigella and Salmonella, Paris, FranceFrench National Reference Centre for Antibiotic Resistance, Le Kremlin-Bicêtre, FranceDepartment of Bacteriology, University Hospital of Clermont-Ferrand, Clermont-Ferrand, FranceBruker Daltonik GmbH, Bremen, GermanyMRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United KingdomMRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United KingdomResistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin resistance. Currently, minimal inhibitory concentration (MIC) determination by broth microdilution, the gold standard for the detection of polymyxin resistance, is time consuming (24 h) and challenging to perform in clinical and veterinary laboratories. Here we present the use of the MALDIxin to detect colistin resistant Salmonella enterica using the MALDxin test on the routine matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system.https://www.frontiersin.org/article/10.3389/fmicb.2020.01141/fullMALDI mass spectrometrylipid AcolistinSalmonella entericadiagno stic |
| spellingShingle | Laurent Dortet Laurent Dortet Laurent Dortet Laurent Dortet Rémy A. Bonnin Rémy A. Bonnin Rémy A. Bonnin Simon Le Hello Laetitia Fabre Richard Bonnet Richard Bonnet Markus Kostrzewa Alain Filloux Gerald Larrouy-Maumus Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer Frontiers in Microbiology MALDI mass spectrometry lipid A colistin Salmonella enterica diagno stic |
| title | Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer |
| title_full | Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer |
| title_fullStr | Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer |
| title_full_unstemmed | Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer |
| title_short | Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer |
| title_sort | detection of colistin resistance in salmonella enterica using maldixin test on the routine maldi biotyper sirius mass spectrometer |
| topic | MALDI mass spectrometry lipid A colistin Salmonella enterica diagno stic |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01141/full |
| work_keys_str_mv | AT laurentdortet detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT laurentdortet detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT laurentdortet detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT laurentdortet detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT remyabonnin detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT remyabonnin detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT remyabonnin detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT simonlehello detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT laetitiafabre detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT richardbonnet detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT richardbonnet detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT markuskostrzewa detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT alainfilloux detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer AT geraldlarrouymaumus detectionofcolistinresistanceinsalmonellaentericausingmaldixintestontheroutinemaldibiotypersiriusmassspectrometer |