MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases
Abstract Background Myxofibrosarcoma (MFS) is one of the most common soft tissue sarcomas. Previous studies have shown that MET protein overexpressed in MFS patients and can serve as a prognostic factor. The reasons for MET protein overexpression include amplification of the MET gene, which is locat...
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | Diagnostic Pathology |
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| Online Access: | http://link.springer.com/article/10.1186/s13000-018-0733-9 |
| _version_ | 1818969942729424896 |
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| author | Shirong Ma Linni Fan Yixiong Liu Yingmei Wang Kangjie Yu Lifeng Wang Na Fang Fang Liu Shuangping Guo Zhe Wang |
| author_facet | Shirong Ma Linni Fan Yixiong Liu Yingmei Wang Kangjie Yu Lifeng Wang Na Fang Fang Liu Shuangping Guo Zhe Wang |
| author_sort | Shirong Ma |
| collection | DOAJ |
| description | Abstract Background Myxofibrosarcoma (MFS) is one of the most common soft tissue sarcomas. Previous studies have shown that MET protein overexpressed in MFS patients and can serve as a prognostic factor. The reasons for MET protein overexpression include amplification of the MET gene, which is located on chromosome 7q. Triggered by an index case harboring chromosome 7 polysomy rather than MET gene amplification in myxofibrosarcoma, we investigated chromosome 7 polysomy in more cases. Methods Immunohistochemistry and fluorescence in situ hybridization (FISH) were performed in 30 MFS cases (including 2 epithelioid variant) to detect the expression of MET protein and gene status. Results MET was overexpressed in 14 cases out of 30, while thirteen cases were in higher FNCLCC grades (Grade 2–3). FISH showed that 11 cases having 3 signals on average of Met and more than 3 signals (Mean: 4.6) of centromere 7q (CEP7q). The MET/CEP7 ratio was about 0.65 on average, suggesting that chromosome 7 polysomy, rather than Met gene amplification, leading to the overexpression of MET protein in MFS. MET overexpression and chromosome 7 polysomy are positively correlated with higher Ki-67 index and higher grade and might have a high risk of local recurrence and metastasis. Conclusions It might reveals another explain of MET overexpression in myxofibrosarcoma, providing a clue for the therapy of MFS. |
| first_indexed | 2024-12-20T14:28:36Z |
| format | Article |
| id | doaj.art-715742c9aade4630b69565f7d0e26f48 |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-12-20T14:28:36Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-715742c9aade4630b69565f7d0e26f482022-12-21T19:37:41ZengBMCDiagnostic Pathology1746-15962018-08-011311810.1186/s13000-018-0733-9MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma casesShirong Ma0Linni Fan1Yixiong Liu2Yingmei Wang3Kangjie Yu4Lifeng Wang5Na Fang6Fang Liu7Shuangping Guo8Zhe Wang9State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical UniversityState Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical UniversityState Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical UniversityState Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical UniversityStudent Team 1, Class 3, Fourth Military Medical UniversityDepartment of Pathology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Pathology, Hubei Provincial Cancer hospitalDepartment of Pathology, Affiliated Foshan hospital, Sun Yet-sen UniversityState Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical UniversityState Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical UniversityAbstract Background Myxofibrosarcoma (MFS) is one of the most common soft tissue sarcomas. Previous studies have shown that MET protein overexpressed in MFS patients and can serve as a prognostic factor. The reasons for MET protein overexpression include amplification of the MET gene, which is located on chromosome 7q. Triggered by an index case harboring chromosome 7 polysomy rather than MET gene amplification in myxofibrosarcoma, we investigated chromosome 7 polysomy in more cases. Methods Immunohistochemistry and fluorescence in situ hybridization (FISH) were performed in 30 MFS cases (including 2 epithelioid variant) to detect the expression of MET protein and gene status. Results MET was overexpressed in 14 cases out of 30, while thirteen cases were in higher FNCLCC grades (Grade 2–3). FISH showed that 11 cases having 3 signals on average of Met and more than 3 signals (Mean: 4.6) of centromere 7q (CEP7q). The MET/CEP7 ratio was about 0.65 on average, suggesting that chromosome 7 polysomy, rather than Met gene amplification, leading to the overexpression of MET protein in MFS. MET overexpression and chromosome 7 polysomy are positively correlated with higher Ki-67 index and higher grade and might have a high risk of local recurrence and metastasis. Conclusions It might reveals another explain of MET overexpression in myxofibrosarcoma, providing a clue for the therapy of MFS.http://link.springer.com/article/10.1186/s13000-018-0733-9MyxofibrosarcomaMETFISHChromosome 7 polysomy |
| spellingShingle | Shirong Ma Linni Fan Yixiong Liu Yingmei Wang Kangjie Yu Lifeng Wang Na Fang Fang Liu Shuangping Guo Zhe Wang MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases Diagnostic Pathology Myxofibrosarcoma MET FISH Chromosome 7 polysomy |
| title | MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases |
| title_full | MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases |
| title_fullStr | MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases |
| title_full_unstemmed | MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases |
| title_short | MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases |
| title_sort | met overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not met amplification especially in high grade cases clinical and pathological review of 30 myxofibrosarcoma cases |
| topic | Myxofibrosarcoma MET FISH Chromosome 7 polysomy |
| url | http://link.springer.com/article/10.1186/s13000-018-0733-9 |
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