Basophil activation test in the diagnosis of allergic reactions to local anesthetics
Objective: to evaluate the detection of in vitro hypersensitivity to local anesthesia drugs by fl ow cytometry.Materials and methods: in the test of activation of basophils, the determination of the hypersensitivity reaction to local anesthetics of groups I, II (154 preparations) in 106 patients and...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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State Budget Educational Institute of Higher Professional Education, Rostov State Medical University, Ministry Health of Russian Federation
2022-03-01
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| Series: | Медицинский вестник Юга России |
| Subjects: | |
| Online Access: | https://www.medicalherald.ru/jour/article/view/1477 |
| Summary: | Objective: to evaluate the detection of in vitro hypersensitivity to local anesthesia drugs by fl ow cytometry.Materials and methods: in the test of activation of basophils, the determination of the hypersensitivity reaction to local anesthetics of groups I, II (154 preparations) in 106 patients and T-lymphocytes of type 2 immune response (CD3 + CD294 +) was carried out by fl ow cytometry using the Allergenicity kit test system. In the history of the examined patients, there was a certain food and/ or drug intolerance.Results: Th e levels of sensitization to diff erent groups of local anesthetics were compared in the basophil activation test. Th e highest levels of hypersensitivity were observed to local anesthetics containing vasoconstrictors, stabilizers. In patients with hypersensitivity to drugs for local anesthesia, an increase in the number of T-lymphocytes of type 2 of the immune response was observed.Conclusions: based on the identifi ed hypersensitivity in the basophil activation test to local anesthesia drugs, the safest anesthetics were established. Th e diagnostic signifi cance of determining the level of T-lymphocytes of type 2 of the immune response in drug hypersensitivity is shown. |
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| ISSN: | 2219-8075 2618-7876 |