Production of optical pure L-lactic acid from Cabernet Sauvignon grape pomace by engineered Lactiplantibacillus plantarum
Cabernet Sauvignon grape pomace contains carbohydrates and various amino acids that could be used as substrates for lactic acid (LA) production. In this study, a mutant strain of L. plantarum with deletion of the D-lactate synthesis gene was developed and used to produce optical pure L-LA from grape...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Energy Research |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenrg.2023.1228827/full |
| _version_ | 1827901018014220288 |
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| author | Yuli Shen Bohua Kang Yi Lu Xinyuan Du Chuan Qin Jing Li Zhaoer Zhao Ran Yu Suan Shi Lujia Han |
| author_facet | Yuli Shen Bohua Kang Yi Lu Xinyuan Du Chuan Qin Jing Li Zhaoer Zhao Ran Yu Suan Shi Lujia Han |
| author_sort | Yuli Shen |
| collection | DOAJ |
| description | Cabernet Sauvignon grape pomace contains carbohydrates and various amino acids that could be used as substrates for lactic acid (LA) production. In this study, a mutant strain of L. plantarum with deletion of the D-lactate synthesis gene was developed and used to produce optical pure L-LA from grape pomace. The highest optical purity of the L-LA produced by this mutant strain was 99.61%. The direct bioconversion of the raw substrate showed a low LA yield. Several pretreatment methods were applied to improve the LA yield, including ball milling, hydrothermal, dilute acid pretreatment, alkaline pretreatment, and combined wet alkaline mechanical pretreatment. Due to the efficient delignification, alkaline pretreatment achieved the highest lactic acid yield of 96.26% at 15% solid loading, with corresponding LA concentration and volumetric productivity of 18.45 g/L and 2.30 g/L·h, respectively. |
| first_indexed | 2024-03-12T23:38:47Z |
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| id | doaj.art-7158f9454f6e4266979afdc2f82720aa |
| institution | Directory Open Access Journal |
| issn | 2296-598X |
| language | English |
| last_indexed | 2024-03-12T23:38:47Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Energy Research |
| spelling | doaj.art-7158f9454f6e4266979afdc2f82720aa2023-07-15T02:26:06ZengFrontiers Media S.A.Frontiers in Energy Research2296-598X2023-07-011110.3389/fenrg.2023.12288271228827Production of optical pure L-lactic acid from Cabernet Sauvignon grape pomace by engineered Lactiplantibacillus plantarumYuli Shen0Bohua Kang1Yi Lu2Xinyuan Du3Chuan Qin4Jing Li5Zhaoer Zhao6Ran Yu7Suan Shi8Lujia Han9Engineering Laboratory for Agro Biomass Recycling and Valorizing, College of Engineering, China Agricultural University, Beijing, ChinaEngineering Laboratory for Agro Biomass Recycling and Valorizing, College of Engineering, China Agricultural University, Beijing, ChinaEngineering Laboratory for Agro Biomass Recycling and Valorizing, College of Engineering, China Agricultural University, Beijing, ChinaEngineering Laboratory for Agro Biomass Recycling and Valorizing, College of Engineering, China Agricultural University, Beijing, ChinaEngineering Laboratory for Agro Biomass Recycling and Valorizing, College of Engineering, China Agricultural University, Beijing, ChinaSchool of Ecology and Environment, Beijing Technology and Business University, Beijing, ChinaKey Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaSino-French Joint Venture Dynasty Winery Ltd., Tianjin, ChinaEngineering Laboratory for Agro Biomass Recycling and Valorizing, College of Engineering, China Agricultural University, Beijing, ChinaEngineering Laboratory for Agro Biomass Recycling and Valorizing, College of Engineering, China Agricultural University, Beijing, ChinaCabernet Sauvignon grape pomace contains carbohydrates and various amino acids that could be used as substrates for lactic acid (LA) production. In this study, a mutant strain of L. plantarum with deletion of the D-lactate synthesis gene was developed and used to produce optical pure L-LA from grape pomace. The highest optical purity of the L-LA produced by this mutant strain was 99.61%. The direct bioconversion of the raw substrate showed a low LA yield. Several pretreatment methods were applied to improve the LA yield, including ball milling, hydrothermal, dilute acid pretreatment, alkaline pretreatment, and combined wet alkaline mechanical pretreatment. Due to the efficient delignification, alkaline pretreatment achieved the highest lactic acid yield of 96.26% at 15% solid loading, with corresponding LA concentration and volumetric productivity of 18.45 g/L and 2.30 g/L·h, respectively.https://www.frontiersin.org/articles/10.3389/fenrg.2023.1228827/fullgrape pomacepretreatmentlactic aciddelignificationCabernet Sauvignon |
| spellingShingle | Yuli Shen Bohua Kang Yi Lu Xinyuan Du Chuan Qin Jing Li Zhaoer Zhao Ran Yu Suan Shi Lujia Han Production of optical pure L-lactic acid from Cabernet Sauvignon grape pomace by engineered Lactiplantibacillus plantarum Frontiers in Energy Research grape pomace pretreatment lactic acid delignification Cabernet Sauvignon |
| title | Production of optical pure L-lactic acid from Cabernet Sauvignon grape pomace by engineered Lactiplantibacillus plantarum |
| title_full | Production of optical pure L-lactic acid from Cabernet Sauvignon grape pomace by engineered Lactiplantibacillus plantarum |
| title_fullStr | Production of optical pure L-lactic acid from Cabernet Sauvignon grape pomace by engineered Lactiplantibacillus plantarum |
| title_full_unstemmed | Production of optical pure L-lactic acid from Cabernet Sauvignon grape pomace by engineered Lactiplantibacillus plantarum |
| title_short | Production of optical pure L-lactic acid from Cabernet Sauvignon grape pomace by engineered Lactiplantibacillus plantarum |
| title_sort | production of optical pure l lactic acid from cabernet sauvignon grape pomace by engineered lactiplantibacillus plantarum |
| topic | grape pomace pretreatment lactic acid delignification Cabernet Sauvignon |
| url | https://www.frontiersin.org/articles/10.3389/fenrg.2023.1228827/full |
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