Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants
The present study aimed to evaluate the effect of supplementation of INRA-82 semen extender with different cryoprotectants (dimethyl sulphoxide; DMSO vs. dimethyl formamide; DMF) on the quality of white New Zealand rabbit buck spermatozoa. We also investigated the possible association between the sy...
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| Format: | Article |
| Language: | English |
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Universitat Politècnica de València
2019-06-01
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| Series: | World Rabbit Science |
| Subjects: | |
| Online Access: | https://polipapers.upv.es/index.php/wrs/article/view/10892 |
| _version_ | 1828450934674423808 |
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| author | Aya Mohamed Fadl Abdel-Raouf Morsy Ghallab Mostafa Mohamed Abou-Ahmed |
| author_facet | Aya Mohamed Fadl Abdel-Raouf Morsy Ghallab Mostafa Mohamed Abou-Ahmed |
| author_sort | Aya Mohamed Fadl |
| collection | DOAJ |
| description | The present study aimed to evaluate the effect of supplementation of INRA-82 semen extender with different cryoprotectants (dimethyl sulphoxide; DMSO vs. dimethyl formamide; DMF) on the quality of white New Zealand rabbit buck spermatozoa. We also investigated the possible association between the synergistic action of DMSO and DMF and their relation with INRA-82 extender composition. Semen was collected and pooled from 8 adult rabbit bucks. Pooled semen samples were diluted 1:1 with INRA-82 extender supplemented with DMSO 8%, DMF 8% or a combination of DMSO 4% and DMF 4%. The diluted semen samples were cryopreserved in 0.25 plastic straws. After thawing, progressive motility, sperm viability, sperm abnormalities, membrane integrity, acrosome status, viability index and DNA integrity were evaluated. The results showed that dilution of rabbit buck semen in INRA-82 supplemented with DMSO and DMF (4% each) before freezing significantly (P<0.05) improved sperm motility (42.00%), percentage of live spermatozoa (45.30%), proportions of spermatozoa with intact acrosome (59.75%) and percentage of spermatozoa with non-fragmented DNA (86.04%), compared to those diluted in INRA-82 supplemented either with DMSO 8% (+9, +10, +5 and +7 percentage points, respectively) or with DMF 8% alone (+18 +18, +12 and +9 percentage points, respectively). In conclusion, dilution of rabbit buck semen before freezing with INRA-82 extender supplemented with a combination of DMSO 4% and DMF 4% improved quality of frozen-thawed New Zealand White rabbit spermatozoa. Furthermore, our results also suggest that supplementation of INRA-82 with DMSO or with DMF alone at higher concentrations deteriorates the sperm quality. |
| first_indexed | 2024-12-10T23:28:56Z |
| format | Article |
| id | doaj.art-715bbbc7319347eb91fe3f61f3c10f4b |
| institution | Directory Open Access Journal |
| issn | 1257-5011 1989-8886 |
| language | English |
| last_indexed | 2024-12-10T23:28:56Z |
| publishDate | 2019-06-01 |
| publisher | Universitat Politècnica de València |
| record_format | Article |
| series | World Rabbit Science |
| spelling | doaj.art-715bbbc7319347eb91fe3f61f3c10f4b2022-12-22T01:29:27ZengUniversitat Politècnica de ValènciaWorld Rabbit Science1257-50111989-88862019-06-01272778310.4995/wrs.2019.108927554Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectantsAya Mohamed Fadl0Abdel-Raouf Morsy Ghallab1Mostafa Mohamed Abou-Ahmed2Cairo UniversityCairo UniversityDepartment of Theriogenology, Faculty of Veterinary MedicineThe present study aimed to evaluate the effect of supplementation of INRA-82 semen extender with different cryoprotectants (dimethyl sulphoxide; DMSO vs. dimethyl formamide; DMF) on the quality of white New Zealand rabbit buck spermatozoa. We also investigated the possible association between the synergistic action of DMSO and DMF and their relation with INRA-82 extender composition. Semen was collected and pooled from 8 adult rabbit bucks. Pooled semen samples were diluted 1:1 with INRA-82 extender supplemented with DMSO 8%, DMF 8% or a combination of DMSO 4% and DMF 4%. The diluted semen samples were cryopreserved in 0.25 plastic straws. After thawing, progressive motility, sperm viability, sperm abnormalities, membrane integrity, acrosome status, viability index and DNA integrity were evaluated. The results showed that dilution of rabbit buck semen in INRA-82 supplemented with DMSO and DMF (4% each) before freezing significantly (P<0.05) improved sperm motility (42.00%), percentage of live spermatozoa (45.30%), proportions of spermatozoa with intact acrosome (59.75%) and percentage of spermatozoa with non-fragmented DNA (86.04%), compared to those diluted in INRA-82 supplemented either with DMSO 8% (+9, +10, +5 and +7 percentage points, respectively) or with DMF 8% alone (+18 +18, +12 and +9 percentage points, respectively). In conclusion, dilution of rabbit buck semen before freezing with INRA-82 extender supplemented with a combination of DMSO 4% and DMF 4% improved quality of frozen-thawed New Zealand White rabbit spermatozoa. Furthermore, our results also suggest that supplementation of INRA-82 with DMSO or with DMF alone at higher concentrations deteriorates the sperm quality.https://polipapers.upv.es/index.php/wrs/article/view/10892rabbit buckcryopreservationINRA-82DMSODMF |
| spellingShingle | Aya Mohamed Fadl Abdel-Raouf Morsy Ghallab Mostafa Mohamed Abou-Ahmed Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants World Rabbit Science rabbit buck cryopreservation INRA-82 DMSO DMF |
| title | Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants |
| title_full | Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants |
| title_fullStr | Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants |
| title_full_unstemmed | Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants |
| title_short | Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants |
| title_sort | quality assessment of cryopreserved new zealand white rabbit spermatozoa in inra 82 extender containing different cryoprotectants |
| topic | rabbit buck cryopreservation INRA-82 DMSO DMF |
| url | https://polipapers.upv.es/index.php/wrs/article/view/10892 |
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