Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus
In cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the sta...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.651060/full |
| _version_ | 1818742083676012544 |
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| author | Maria Nordengrün Goran Abdurrahman Janina Treffon Janina Treffon Hannah Wächter Barbara C. Kahl Barbara M. Bröker |
| author_facet | Maria Nordengrün Goran Abdurrahman Janina Treffon Janina Treffon Hannah Wächter Barbara C. Kahl Barbara M. Bröker |
| author_sort | Maria Nordengrün |
| collection | DOAJ |
| description | In cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the staphylococcal serine protease-like proteins (Spls) can induce type 2 immune responses in humans and mice. We measured the serum IgE levels specific to 7 proteases of S. aureus by ELISA, targeting 5 Spls (76 CF patients and 46 controls) and the staphopains A and B (16 CF patients and 46 controls). Then we compared cytokine release and phenotype of T cells that had been stimulated with Spls between 5 CF patients and 5 controls. CF patients had strongly increased serum IgE binding to all Spls but not to the staphopains. Compared to healthy controls, their Spl-stimulated T cells released more type 2 cytokines (IL-4, IL-5, IL-13) and more IL-6 with no difference in the secretion of type 1- or type 3 cytokines (IFNγ, IL-17A, IL-17F). IL-10 production was low in CF T cells. The phenotype of the Spl-exposed T cells shifted towards a Th2 or Th17 profile in CF but to a Th1 profile in controls. Sensitization to S. aureus Spls is common in CF. This discovery could explain episodes of allergic inflammation of hitherto unknown causation in CF and extend the diagnostic and therapeutic portfolio. |
| first_indexed | 2024-12-18T02:06:53Z |
| format | Article |
| id | doaj.art-715ca3e6ed52480f8c40a135bab85e07 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-18T02:06:53Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-715ca3e6ed52480f8c40a135bab85e072022-12-21T21:24:34ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-03-011210.3389/fimmu.2021.651060651060Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureusMaria Nordengrün0Goran Abdurrahman1Janina Treffon2Janina Treffon3Hannah Wächter4Barbara C. Kahl5Barbara M. Bröker6Department of Immunology, Institute for Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, GermanyDepartment of Immunology, Institute for Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, GermanyInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyInstitute of Hygiene, University Hospital Münster, Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyDepartment of Immunology, Institute for Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, GermanyIn cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the staphylococcal serine protease-like proteins (Spls) can induce type 2 immune responses in humans and mice. We measured the serum IgE levels specific to 7 proteases of S. aureus by ELISA, targeting 5 Spls (76 CF patients and 46 controls) and the staphopains A and B (16 CF patients and 46 controls). Then we compared cytokine release and phenotype of T cells that had been stimulated with Spls between 5 CF patients and 5 controls. CF patients had strongly increased serum IgE binding to all Spls but not to the staphopains. Compared to healthy controls, their Spl-stimulated T cells released more type 2 cytokines (IL-4, IL-5, IL-13) and more IL-6 with no difference in the secretion of type 1- or type 3 cytokines (IFNγ, IL-17A, IL-17F). IL-10 production was low in CF T cells. The phenotype of the Spl-exposed T cells shifted towards a Th2 or Th17 profile in CF but to a Th1 profile in controls. Sensitization to S. aureus Spls is common in CF. This discovery could explain episodes of allergic inflammation of hitherto unknown causation in CF and extend the diagnostic and therapeutic portfolio.https://www.frontiersin.org/articles/10.3389/fimmu.2021.651060/fullcystic fibrosisStaphylococcus aureusallergytype 2 immune responseIgETh2 cells |
| spellingShingle | Maria Nordengrün Goran Abdurrahman Janina Treffon Janina Treffon Hannah Wächter Barbara C. Kahl Barbara M. Bröker Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus Frontiers in Immunology cystic fibrosis Staphylococcus aureus allergy type 2 immune response IgE Th2 cells |
| title | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
| title_full | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
| title_fullStr | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
| title_full_unstemmed | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
| title_short | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
| title_sort | allergic reactions to serine protease like proteins of staphylococcus aureus |
| topic | cystic fibrosis Staphylococcus aureus allergy type 2 immune response IgE Th2 cells |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.651060/full |
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