Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes
Possible immunogenicity of the Cas9 protein raises concerns about therapeutic applications. Here the authors identify pre-existing CD8+T-cell immunity in healthy individuals and in response modify Cas9 to remove the immunodominant epitopes.
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-09693-x |
| _version_ | 1819036108796723200 |
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| author | Shayesteh R. Ferdosi Radwa Ewaisha Farzaneh Moghadam Sri Krishna Jin G. Park Mo R. Ebrahimkhani Samira Kiani Karen S. Anderson |
| author_facet | Shayesteh R. Ferdosi Radwa Ewaisha Farzaneh Moghadam Sri Krishna Jin G. Park Mo R. Ebrahimkhani Samira Kiani Karen S. Anderson |
| author_sort | Shayesteh R. Ferdosi |
| collection | DOAJ |
| description | Possible immunogenicity of the Cas9 protein raises concerns about therapeutic applications. Here the authors identify pre-existing CD8+T-cell immunity in healthy individuals and in response modify Cas9 to remove the immunodominant epitopes. |
| first_indexed | 2024-12-21T08:00:17Z |
| format | Article |
| id | doaj.art-7163fcf8bec84bbaa28f45dd3cb383d7 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-21T08:00:17Z |
| publishDate | 2019-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-7163fcf8bec84bbaa28f45dd3cb383d72022-12-21T19:10:54ZengNature PortfolioNature Communications2041-17232019-04-0110111010.1038/s41467-019-09693-xMultifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopesShayesteh R. Ferdosi0Radwa Ewaisha1Farzaneh Moghadam2Sri Krishna3Jin G. Park4Mo R. Ebrahimkhani5Samira Kiani6Karen S. Anderson7Center for Personalized Diagnostics, Biodesign Institute, Arizona State UniversityCenter for Personalized Diagnostics, Biodesign Institute, Arizona State UniversitySchool of Biological and Health Systems Engineering, Arizona State UniversityCenter for Personalized Diagnostics, Biodesign Institute, Arizona State UniversityCenter for Personalized Diagnostics, Biodesign Institute, Arizona State UniversitySchool of Biological and Health Systems Engineering, Arizona State UniversitySchool of Biological and Health Systems Engineering, Arizona State UniversityCenter for Personalized Diagnostics, Biodesign Institute, Arizona State UniversityPossible immunogenicity of the Cas9 protein raises concerns about therapeutic applications. Here the authors identify pre-existing CD8+T-cell immunity in healthy individuals and in response modify Cas9 to remove the immunodominant epitopes.https://doi.org/10.1038/s41467-019-09693-x |
| spellingShingle | Shayesteh R. Ferdosi Radwa Ewaisha Farzaneh Moghadam Sri Krishna Jin G. Park Mo R. Ebrahimkhani Samira Kiani Karen S. Anderson Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes Nature Communications |
| title | Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes |
| title_full | Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes |
| title_fullStr | Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes |
| title_full_unstemmed | Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes |
| title_short | Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes |
| title_sort | multifunctional crispr cas9 with engineered immunosilenced human t cell epitopes |
| url | https://doi.org/10.1038/s41467-019-09693-x |
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