The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis
Abstract Background The MYB transcription factor family is one of the largest transcriptional factor families in plants and plays a multifaceted role in plant growth and development. However, MYB transcription factors involved in pathogen resistance in apple remain poorly understood. Results We iden...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1918-4 |
| _version_ | 1818280236870008832 |
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| author | Ya-Li Zhang Chun-Ling Zhang Gui-Luan Wang Yong-Xu Wang Chen-Hui Qi Qiang Zhao Chun-Xiang You Yuan-Yuan Li Yu-Jin Hao |
| author_facet | Ya-Li Zhang Chun-Ling Zhang Gui-Luan Wang Yong-Xu Wang Chen-Hui Qi Qiang Zhao Chun-Xiang You Yuan-Yuan Li Yu-Jin Hao |
| author_sort | Ya-Li Zhang |
| collection | DOAJ |
| description | Abstract Background The MYB transcription factor family is one of the largest transcriptional factor families in plants and plays a multifaceted role in plant growth and development. However, MYB transcription factors involved in pathogen resistance in apple remain poorly understood. Results We identified a new MYB family member from apple, and named it MdMYB30. MdMYB30 was localized to the nucleus, and was highly expressed in young apple leaves. Transcription of MdMYB30 was induced by abiotic stressors, such as polyethylene glycol and abscisic acid. Scanning electron microscopy and gas chromatograph–mass spectrometry analyses demonstrated that ectopically expressing MdMYB30 in Arabidopsis changed the wax content, the number of wax crystals, and the transcription of wax-related genes. MdMYB30 bound to the MdKCS1 promoter to activate its expression and regulate wax biosynthesis. MdMYB30 also contributed to plant surface properties and increased resistance to the bacterial strain Pst DC3000. Furthermore, a virus-based transformation in apple fruits and transgenic apple calli demonstrated that MdMYB30 increased resistance to Botryosphaeria dothidea. Our findings suggest that MdMYB30 plays a vital role in the accumulation of cuticular wax and enhances disease resistance in apple. Conclusions MdMYB30 bound to the MdKCS1 gene promoter to activate its transcription and regulate cuticular wax content and composition, which influenced the surface properties and expression of pathogenesis-related genes to resistance against pathogens. MdMYB30 appears to be a crucial element in the formation of the plant cuticle and confers apple with a tolerance to pathogens. |
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| language | English |
| last_indexed | 2024-12-12T23:46:01Z |
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| series | BMC Plant Biology |
| spelling | doaj.art-7165fb146d6b4215bf8e2780a63f73b22022-12-22T00:06:51ZengBMCBMC Plant Biology1471-22292019-08-0119111410.1186/s12870-019-1918-4The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesisYa-Li Zhang0Chun-Ling Zhang1Gui-Luan Wang2Yong-Xu Wang3Chen-Hui Qi4Qiang Zhao5Chun-Xiang You6Yuan-Yuan Li7Yu-Jin Hao8National Key Laboratory of Crop Biology; Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural UniversityNational Key Laboratory of Crop Biology; Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural UniversityNational Key Laboratory of Crop Biology; Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural UniversityNational Key Laboratory of Crop Biology; Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural UniversityNational Key Laboratory of Crop Biology; Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural UniversityNational Key Laboratory of Crop Biology; Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural UniversityNational Key Laboratory of Crop Biology; Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural UniversityNational Key Laboratory of Crop Biology; Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural UniversityNational Key Laboratory of Crop Biology; Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production; College of Horticulture Science and Engineering, Shandong Agricultural UniversityAbstract Background The MYB transcription factor family is one of the largest transcriptional factor families in plants and plays a multifaceted role in plant growth and development. However, MYB transcription factors involved in pathogen resistance in apple remain poorly understood. Results We identified a new MYB family member from apple, and named it MdMYB30. MdMYB30 was localized to the nucleus, and was highly expressed in young apple leaves. Transcription of MdMYB30 was induced by abiotic stressors, such as polyethylene glycol and abscisic acid. Scanning electron microscopy and gas chromatograph–mass spectrometry analyses demonstrated that ectopically expressing MdMYB30 in Arabidopsis changed the wax content, the number of wax crystals, and the transcription of wax-related genes. MdMYB30 bound to the MdKCS1 promoter to activate its expression and regulate wax biosynthesis. MdMYB30 also contributed to plant surface properties and increased resistance to the bacterial strain Pst DC3000. Furthermore, a virus-based transformation in apple fruits and transgenic apple calli demonstrated that MdMYB30 increased resistance to Botryosphaeria dothidea. Our findings suggest that MdMYB30 plays a vital role in the accumulation of cuticular wax and enhances disease resistance in apple. Conclusions MdMYB30 bound to the MdKCS1 gene promoter to activate its transcription and regulate cuticular wax content and composition, which influenced the surface properties and expression of pathogenesis-related genes to resistance against pathogens. MdMYB30 appears to be a crucial element in the formation of the plant cuticle and confers apple with a tolerance to pathogens.http://link.springer.com/article/10.1186/s12870-019-1918-4AppleCuticular waxMdMYB30Pathogens resistance |
| spellingShingle | Ya-Li Zhang Chun-Ling Zhang Gui-Luan Wang Yong-Xu Wang Chen-Hui Qi Qiang Zhao Chun-Xiang You Yuan-Yuan Li Yu-Jin Hao The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis BMC Plant Biology Apple Cuticular wax MdMYB30 Pathogens resistance |
| title | The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis |
| title_full | The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis |
| title_fullStr | The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis |
| title_full_unstemmed | The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis |
| title_short | The R2R3 MYB transcription factor MdMYB30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis |
| title_sort | r2r3 myb transcription factor mdmyb30 modulates plant resistance against pathogens by regulating cuticular wax biosynthesis |
| topic | Apple Cuticular wax MdMYB30 Pathogens resistance |
| url | http://link.springer.com/article/10.1186/s12870-019-1918-4 |
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