Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts
Curative bone marrow transplantation (BMT) therapies for sickle cell disease (SCD) can cause infertility. The Fertility Preservation Program (FPP) in Pittsburgh cryopreserves testicular tissues for SCD patients prior to BMT in anticipation that those tissues can be thawed in the future and matured t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350612300243X |
| _version_ | 1797401757032120320 |
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| author | Mitchell R. Harancher Meena Sukhwani Carlos A. Castro Michael H. Hsieh Kyle E. Orwig |
| author_facet | Mitchell R. Harancher Meena Sukhwani Carlos A. Castro Michael H. Hsieh Kyle E. Orwig |
| author_sort | Mitchell R. Harancher |
| collection | DOAJ |
| description | Curative bone marrow transplantation (BMT) therapies for sickle cell disease (SCD) can cause infertility. The Fertility Preservation Program (FPP) in Pittsburgh cryopreserves testicular tissues for SCD patients prior to BMT in anticipation that those tissues can be thawed in the future and matured to produce sperm. Here, we generated and validated two isogenic patient-derived induced pluripotent stem cell (iPSC) lines from testicular biopsy fibroblasts of a 12-year-old SCD patient. |
| first_indexed | 2024-03-09T02:14:44Z |
| format | Article |
| id | doaj.art-7166c5f4e1a648bcacc6878ea1ae3812 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-09T02:14:44Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-7166c5f4e1a648bcacc6878ea1ae38122023-12-07T05:28:25ZengElsevierStem Cell Research1873-50612023-12-0173103257Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblastsMitchell R. Harancher0Meena Sukhwani1Carlos A. Castro2Michael H. Hsieh3Kyle E. Orwig4Molecular Genetics and Developmental Biology Graduate Program, University of Pittsburgh School of Medicine, USA; Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USADepartment of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USADepartment of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USAChildren’s National Hospital, Washington, DC, USAMolecular Genetics and Developmental Biology Graduate Program, University of Pittsburgh School of Medicine, USA; Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, USA; Corresponding author.Curative bone marrow transplantation (BMT) therapies for sickle cell disease (SCD) can cause infertility. The Fertility Preservation Program (FPP) in Pittsburgh cryopreserves testicular tissues for SCD patients prior to BMT in anticipation that those tissues can be thawed in the future and matured to produce sperm. Here, we generated and validated two isogenic patient-derived induced pluripotent stem cell (iPSC) lines from testicular biopsy fibroblasts of a 12-year-old SCD patient.http://www.sciencedirect.com/science/article/pii/S187350612300243X |
| spellingShingle | Mitchell R. Harancher Meena Sukhwani Carlos A. Castro Michael H. Hsieh Kyle E. Orwig Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts Stem Cell Research |
| title | Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts |
| title_full | Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts |
| title_fullStr | Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts |
| title_full_unstemmed | Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts |
| title_short | Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts |
| title_sort | generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts |
| url | http://www.sciencedirect.com/science/article/pii/S187350612300243X |
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