A novel long non-coding RNA SLNCR1 promotes proliferation, migration, and invasion of melanoma via transcriptionally regulating SOX5
Abstract Steroid receptor RNA activator (SRA)-like non-coding RNA (SLNCR1) has been implicated in various tumorigenic processes, but the precise regulatory role in melanoma progression remains uncertain. We performed a comprehensive analysis to investigate the prognostic value of SLNCR1 expression i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2024-04-01
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| Series: | Cell Death Discovery |
| Online Access: | https://doi.org/10.1038/s41420-024-01922-7 |
| _version_ | 1797220003192242176 |
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| author | Lele Cong Qing Zhao Hongyan Sun Zilong Zhou Yue Hu Chunyi Li Miao Hao Xianling Cong |
| author_facet | Lele Cong Qing Zhao Hongyan Sun Zilong Zhou Yue Hu Chunyi Li Miao Hao Xianling Cong |
| author_sort | Lele Cong |
| collection | DOAJ |
| description | Abstract Steroid receptor RNA activator (SRA)-like non-coding RNA (SLNCR1) has been implicated in various tumorigenic processes, but the precise regulatory role in melanoma progression remains uncertain. We performed a comprehensive analysis to investigate the prognostic value of SLNCR1 expression in patients with melanoma by TCGA database and melanoma tissue samples via the Kaplan–Meier method. Subsequently, we conducted qRT-PCR and Fluorescence in Situ Hybridization (FISH) assays to identify SLNCR1 expression levels and localization in tissues and cells, respectively. Loss-of-function assays utilizing shRNAs vectors were used to investigate the potential impact of SLNCR1. Our data showed that SLNCR1 is significantly up-regulated in human malignant melanoma tissues and cell lines and functions as an oncogene. Silencing of SLNCR1 suppressed melanoma cell proliferation, migration, invasion, and inhibited tumorigenesis in a mouse xenograft model. Additionally, we employed bioinformatic predictive analysis, combined with dual-luciferase reporter analysis and functional rescue assays, to elucidate the mechanistic target of the SLNCR1/SOX5 axis in melanoma. Mechanistically, we discovered that SLNCR1 promotes EMT of human melanoma by targeting SOX5, as downregulation of SLNCR1 expression leads to a decrease in SOX5 protein levels and inhibits melanoma tumorigenesis. Our research offers promising insights for more precise diagnosis and treatment of human melanoma. |
| first_indexed | 2024-04-24T12:42:37Z |
| format | Article |
| id | doaj.art-7167a52d4c424b5a90d3ffad51a0c47d |
| institution | Directory Open Access Journal |
| issn | 2058-7716 |
| language | English |
| last_indexed | 2024-04-24T12:42:37Z |
| publishDate | 2024-04-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death Discovery |
| spelling | doaj.art-7167a52d4c424b5a90d3ffad51a0c47d2024-04-07T11:08:32ZengNature Publishing GroupCell Death Discovery2058-77162024-04-0110111110.1038/s41420-024-01922-7A novel long non-coding RNA SLNCR1 promotes proliferation, migration, and invasion of melanoma via transcriptionally regulating SOX5Lele Cong0Qing Zhao1Hongyan Sun2Zilong Zhou3Yue Hu4Chunyi Li5Miao Hao6Xianling Cong7Department of Dermatology, China-Japan Union Hospital of Jilin UniversityDepartment of Neurology, China-Japan Union Hospital of Jilin UniversityDepartment of Biobank, China-Japan Union Hospital of Jilin UniversityDepartment of Biobank, China-Japan Union Hospital of Jilin UniversityDepartment of Biobank, China-Japan Union Hospital of Jilin UniversityInstitute of Antler Science and Product Technology, Changchun Sci-Tech UniversityScientific Research Center, China-Japan Union Hospital of Jilin UniversityDepartment of Biobank, China-Japan Union Hospital of Jilin UniversityAbstract Steroid receptor RNA activator (SRA)-like non-coding RNA (SLNCR1) has been implicated in various tumorigenic processes, but the precise regulatory role in melanoma progression remains uncertain. We performed a comprehensive analysis to investigate the prognostic value of SLNCR1 expression in patients with melanoma by TCGA database and melanoma tissue samples via the Kaplan–Meier method. Subsequently, we conducted qRT-PCR and Fluorescence in Situ Hybridization (FISH) assays to identify SLNCR1 expression levels and localization in tissues and cells, respectively. Loss-of-function assays utilizing shRNAs vectors were used to investigate the potential impact of SLNCR1. Our data showed that SLNCR1 is significantly up-regulated in human malignant melanoma tissues and cell lines and functions as an oncogene. Silencing of SLNCR1 suppressed melanoma cell proliferation, migration, invasion, and inhibited tumorigenesis in a mouse xenograft model. Additionally, we employed bioinformatic predictive analysis, combined with dual-luciferase reporter analysis and functional rescue assays, to elucidate the mechanistic target of the SLNCR1/SOX5 axis in melanoma. Mechanistically, we discovered that SLNCR1 promotes EMT of human melanoma by targeting SOX5, as downregulation of SLNCR1 expression leads to a decrease in SOX5 protein levels and inhibits melanoma tumorigenesis. Our research offers promising insights for more precise diagnosis and treatment of human melanoma.https://doi.org/10.1038/s41420-024-01922-7 |
| spellingShingle | Lele Cong Qing Zhao Hongyan Sun Zilong Zhou Yue Hu Chunyi Li Miao Hao Xianling Cong A novel long non-coding RNA SLNCR1 promotes proliferation, migration, and invasion of melanoma via transcriptionally regulating SOX5 Cell Death Discovery |
| title | A novel long non-coding RNA SLNCR1 promotes proliferation, migration, and invasion of melanoma via transcriptionally regulating SOX5 |
| title_full | A novel long non-coding RNA SLNCR1 promotes proliferation, migration, and invasion of melanoma via transcriptionally regulating SOX5 |
| title_fullStr | A novel long non-coding RNA SLNCR1 promotes proliferation, migration, and invasion of melanoma via transcriptionally regulating SOX5 |
| title_full_unstemmed | A novel long non-coding RNA SLNCR1 promotes proliferation, migration, and invasion of melanoma via transcriptionally regulating SOX5 |
| title_short | A novel long non-coding RNA SLNCR1 promotes proliferation, migration, and invasion of melanoma via transcriptionally regulating SOX5 |
| title_sort | novel long non coding rna slncr1 promotes proliferation migration and invasion of melanoma via transcriptionally regulating sox5 |
| url | https://doi.org/10.1038/s41420-024-01922-7 |
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