Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification
Abstract Background Improving lignocellulolytic enzymes’ diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification. Although many chemical features are considered as detrimental to saccharification, enzymes’ dynamics with...
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-018-1267-9 |
| _version_ | 1818264689449107456 |
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| author | Mickaël Herbaut Aya Zoghlami Gabriel Paës |
| author_facet | Mickaël Herbaut Aya Zoghlami Gabriel Paës |
| author_sort | Mickaël Herbaut |
| collection | DOAJ |
| description | Abstract Background Improving lignocellulolytic enzymes’ diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification. Although many chemical features are considered as detrimental to saccharification, enzymes’ dynamics within the cell walls remains poorly explored and understood. To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose. In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification. Results Probes’ diffusion was mainly affected by probes size and pretreatments but only slightly by saccharification time. This means that, despite the removal of polysaccharides during saccharification, diffusion of probes was not improved since they became hindered by changes in lignin conformation, whose relative amount increased over time. Porosity measurements showed that probes’ diffusion was highly correlated with the amount of pores having a diameter at least five times the size of the probes. Testing the relationship with saccharification demonstrated that accessibility of 1.3–1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5–20 nm. Conclusion Mobility measurements performed before hydrolysis can serve to explain and even predict saccharification with accuracy. The discrepancy observed between probes’ size and pores’ diameters to explain accessibility is likely due to biomass features such as lignin content and composition that prevent probes’ diffusion through non-specific interactions probably leading to pores’ entanglements. |
| first_indexed | 2024-12-12T19:38:54Z |
| format | Article |
| id | doaj.art-716b92a2777d4322a28aebef920e7058 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T19:38:54Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-716b92a2777d4322a28aebef920e70582022-12-22T00:14:15ZengBMCBiotechnology for Biofuels1754-68342018-10-0111111310.1186/s13068-018-1267-9Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharificationMickaël Herbaut0Aya Zoghlami1Gabriel Paës2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, University of Reims Champagne-ArdenneFractionation of AgroResources and Environment (FARE) Laboratory, INRA, University of Reims Champagne-ArdenneFractionation of AgroResources and Environment (FARE) Laboratory, INRA, University of Reims Champagne-ArdenneAbstract Background Improving lignocellulolytic enzymes’ diffusion and accessibility to their substrate in the plant cell walls is recognised as a critical issue for optimising saccharification. Although many chemical features are considered as detrimental to saccharification, enzymes’ dynamics within the cell walls remains poorly explored and understood. To address this issue, poplar fragments were submitted to hot water and ionic liquid pretreatments selected for their contrasted effects on both the structure and composition of lignocellulose. In addition to chemical composition and porosity analyses, the diffusion of polyethylene glycol probes of different sizes was measured at three different time points during the saccharification. Results Probes’ diffusion was mainly affected by probes size and pretreatments but only slightly by saccharification time. This means that, despite the removal of polysaccharides during saccharification, diffusion of probes was not improved since they became hindered by changes in lignin conformation, whose relative amount increased over time. Porosity measurements showed that probes’ diffusion was highly correlated with the amount of pores having a diameter at least five times the size of the probes. Testing the relationship with saccharification demonstrated that accessibility of 1.3–1.7-nm radius probes measured by FRAP on non-hydrolysed samples was highly correlated with poplar digestibility together with the measurement of initial porosity on the range 5–20 nm. Conclusion Mobility measurements performed before hydrolysis can serve to explain and even predict saccharification with accuracy. The discrepancy observed between probes’ size and pores’ diameters to explain accessibility is likely due to biomass features such as lignin content and composition that prevent probes’ diffusion through non-specific interactions probably leading to pores’ entanglements.http://link.springer.com/article/10.1186/s13068-018-1267-9BiomassPretreatmentSaccharificationPEG-rhodamineFRAPAccessibility |
| spellingShingle | Mickaël Herbaut Aya Zoghlami Gabriel Paës Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification Biotechnology for Biofuels Biomass Pretreatment Saccharification PEG-rhodamine FRAP Accessibility |
| title | Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification |
| title_full | Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification |
| title_fullStr | Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification |
| title_full_unstemmed | Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification |
| title_short | Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification |
| title_sort | dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification |
| topic | Biomass Pretreatment Saccharification PEG-rhodamine FRAP Accessibility |
| url | http://link.springer.com/article/10.1186/s13068-018-1267-9 |
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