Prospective fecal microbiomic biomarkers for chronic wasting disease
ABSTRACTChronic wasting disease (CWD) is a naturally occurring prion disease in cervids that has been rapidly proliferating in the United States. Here, we investigated a potential link between CWD infection and gut microbiome by analyzing 50 fecal samples obtained from CWD-positive animals of differ...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2024-03-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.03750-22 |
| _version_ | 1797276746348756992 |
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| author | Adam Didier Maureen Bourner Guy Kleks Avihai Zolty Brajendra Kumar Tracy Nichols Karie Durynski Susan Bender Michelle Gibison Lisa Murphy Julie C. Ellis Dawei W. Dong Anna Kashina |
| author_facet | Adam Didier Maureen Bourner Guy Kleks Avihai Zolty Brajendra Kumar Tracy Nichols Karie Durynski Susan Bender Michelle Gibison Lisa Murphy Julie C. Ellis Dawei W. Dong Anna Kashina |
| author_sort | Adam Didier |
| collection | DOAJ |
| description | ABSTRACTChronic wasting disease (CWD) is a naturally occurring prion disease in cervids that has been rapidly proliferating in the United States. Here, we investigated a potential link between CWD infection and gut microbiome by analyzing 50 fecal samples obtained from CWD-positive animals of different sexes from various regions in the USA compared to 50 CWD-negative controls using high throughput sequencing of the 16S ribosomal RNA and targeted metabolomics. Our analysis reveals promising trends in the gut microbiota that could potentially be CWD-dependent, including several bacterial taxa at each rank level, as well as taxa pairs, that can differentiate between CWD-negative and CWD-positive deer. Through machine-learning, these taxa and taxa pairs at each rank level could facilitate identification of around 70% of both the CWD-negative and the CWD-positive samples. Our results provide a potential tool for diagnostics and surveillance of CWD in the wild, as well as conceptual advances in our understanding of the disease.IMPORTANCEThis is a comprehensive study that tests the connection between the composition of the gut microbiome in deer in response to chronic wasting disease (CWD). We analyzed 50 fecal samples obtained from CWD-positive animals compared to 50 CWD-negative controls to identify CWD-dependent changes in the gut microbiome, matched with the analysis of fecal metabolites. Our results show promising trends suggesting that fecal microbial composition can directly correspond to CWD disease status. These results point to the microbial composition of the feces as a potential tool for diagnostics and surveillance of CWD in the wild, including non-invasive CWD detection in asymptomatic deer and deer habitats, and enable conceptual advances in our understanding of the disease. |
| first_indexed | 2024-03-07T15:33:43Z |
| format | Article |
| id | doaj.art-71722ea04f7245ad826cea730dfa38f3 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-07T15:33:43Z |
| publishDate | 2024-03-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-71722ea04f7245ad826cea730dfa38f32024-03-05T14:04:36ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-03-0112310.1128/spectrum.03750-22Prospective fecal microbiomic biomarkers for chronic wasting diseaseAdam Didier0Maureen Bourner1Guy Kleks2Avihai Zolty3Brajendra Kumar4Tracy Nichols5Karie Durynski6Susan Bender7Michelle Gibison8Lisa Murphy9Julie C. Ellis10Dawei W. Dong11Anna Kashina12MilliporeSigma, Merck KGaA, Darmstadt, GermanyMilliporeSigma, Merck KGaA, Darmstadt, GermanySigma Aldrich Israel Ltd., Merck KGaA, Darmstadt, GermanySigma Aldrich Israel Ltd., Merck KGaA, Darmstadt, GermanySigma Aldrich Chemical Pvt. Ltd., Merck KGaA, Darmstadt, GermanyUnited States Department of Agriculture, Washington, DC, USASchool of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USASchool of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USAWildlife Futures Program, Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USAWildlife Futures Program, Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USAWildlife Futures Program, Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USASchool of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USASchool of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USAABSTRACTChronic wasting disease (CWD) is a naturally occurring prion disease in cervids that has been rapidly proliferating in the United States. Here, we investigated a potential link between CWD infection and gut microbiome by analyzing 50 fecal samples obtained from CWD-positive animals of different sexes from various regions in the USA compared to 50 CWD-negative controls using high throughput sequencing of the 16S ribosomal RNA and targeted metabolomics. Our analysis reveals promising trends in the gut microbiota that could potentially be CWD-dependent, including several bacterial taxa at each rank level, as well as taxa pairs, that can differentiate between CWD-negative and CWD-positive deer. Through machine-learning, these taxa and taxa pairs at each rank level could facilitate identification of around 70% of both the CWD-negative and the CWD-positive samples. Our results provide a potential tool for diagnostics and surveillance of CWD in the wild, as well as conceptual advances in our understanding of the disease.IMPORTANCEThis is a comprehensive study that tests the connection between the composition of the gut microbiome in deer in response to chronic wasting disease (CWD). We analyzed 50 fecal samples obtained from CWD-positive animals compared to 50 CWD-negative controls to identify CWD-dependent changes in the gut microbiome, matched with the analysis of fecal metabolites. Our results show promising trends suggesting that fecal microbial composition can directly correspond to CWD disease status. These results point to the microbial composition of the feces as a potential tool for diagnostics and surveillance of CWD in the wild, including non-invasive CWD detection in asymptomatic deer and deer habitats, and enable conceptual advances in our understanding of the disease.https://journals.asm.org/doi/10.1128/spectrum.03750-22fecal microbiomicsprionfecal metabolomicsdiagnosticschronic wasting disease |
| spellingShingle | Adam Didier Maureen Bourner Guy Kleks Avihai Zolty Brajendra Kumar Tracy Nichols Karie Durynski Susan Bender Michelle Gibison Lisa Murphy Julie C. Ellis Dawei W. Dong Anna Kashina Prospective fecal microbiomic biomarkers for chronic wasting disease Microbiology Spectrum fecal microbiomics prion fecal metabolomics diagnostics chronic wasting disease |
| title | Prospective fecal microbiomic biomarkers for chronic wasting disease |
| title_full | Prospective fecal microbiomic biomarkers for chronic wasting disease |
| title_fullStr | Prospective fecal microbiomic biomarkers for chronic wasting disease |
| title_full_unstemmed | Prospective fecal microbiomic biomarkers for chronic wasting disease |
| title_short | Prospective fecal microbiomic biomarkers for chronic wasting disease |
| title_sort | prospective fecal microbiomic biomarkers for chronic wasting disease |
| topic | fecal microbiomics prion fecal metabolomics diagnostics chronic wasting disease |
| url | https://journals.asm.org/doi/10.1128/spectrum.03750-22 |
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