BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA
The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resis...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Scientific Сentre for Family Health and Human Reproduction Problems
2018-09-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/706 |
| _version_ | 1797237341656449024 |
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| author | A. Yu. Borisenko Yu. P. Dzhioev N. P. Peretolchina L. A. Stepanenko V. A. Kuzminova Yu. M. Zemlyanskaya L. A. Kokorina N. A. Arefieva O. N. Reva Y. Wang Z. Qu V. I. Zlobin |
| author_facet | A. Yu. Borisenko Yu. P. Dzhioev N. P. Peretolchina L. A. Stepanenko V. A. Kuzminova Yu. M. Zemlyanskaya L. A. Kokorina N. A. Arefieva O. N. Reva Y. Wang Z. Qu V. I. Zlobin |
| author_sort | A. Yu. Borisenko |
| collection | DOAJ |
| description | The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resistant Staphylococcus aureus. These antibiotic resistance in common pathogens have made antimicrobial therapy of many infections. Scientists need to look for new ways of treating bacterial infections in the work, using the developed algorithm from the methods of search software in the genomic structure of Staphylococcus aureus subsp. aureus ST228, the CRISPR/Cas locus and the division structures of its CRISPR cassette. The results of the bacteriophage search through the decoded spacer sequences of CRISPR-cassettes of this strain were also obtained using the developed algorithm of the software methods of bioinformatics. It was determined that the CRISPR/Cas system of strain of ST228 of S. aureus was of type IIIA. It is shown that cas-genes are in the immediate vicinity of CRISPR cassettes. The spacer structures in the detected CRISPR cassette are the Staphylococcus, Mycobacterium, Streptococcus, Bacillus, Gordonia, Arthrobacter, Streptomyces. The implementation of the algorithm of program methods for locating CRISPR/Cas-loci can be applied to many other decoded bacterial genomes to return bacteriophage therapy. |
| first_indexed | 2024-03-08T12:18:03Z |
| format | Article |
| id | doaj.art-7172ef98e787428b9ecc43e4d8acfdb1 |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:18:13Z |
| publishDate | 2018-09-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-7172ef98e787428b9ecc43e4d8acfdb12024-03-28T07:49:20ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962018-09-0135495310.29413/ABS.2018-3.5.7666BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIAA. Yu. Borisenko0Yu. P. Dzhioev1N. P. Peretolchina2L. A. Stepanenko3V. A. Kuzminova4Yu. M. Zemlyanskaya5L. A. Kokorina6N. A. Arefieva7O. N. Reva8Y. Wang9Z. Qu10V. I. Zlobin11Irkutsk State Medical University.Irkutsk State Medical University.Irkutsk State Medical University.Irkutsk State Medical University.Irkutsk State University.Irkutsk State Medical University.Irkutsk State Medical University.Irkutsk State University.Centre for Bioinformatics and Computational Biology, Department of Biochemistry, Genetics and Microbiology, University of Pretoria.Department of Microbiology, Harbin Medical University.Department of Microbiology, Harbin Medical University.Irkutsk State Medical University.The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resistant Staphylococcus aureus. These antibiotic resistance in common pathogens have made antimicrobial therapy of many infections. Scientists need to look for new ways of treating bacterial infections in the work, using the developed algorithm from the methods of search software in the genomic structure of Staphylococcus aureus subsp. aureus ST228, the CRISPR/Cas locus and the division structures of its CRISPR cassette. The results of the bacteriophage search through the decoded spacer sequences of CRISPR-cassettes of this strain were also obtained using the developed algorithm of the software methods of bioinformatics. It was determined that the CRISPR/Cas system of strain of ST228 of S. aureus was of type IIIA. It is shown that cas-genes are in the immediate vicinity of CRISPR cassettes. The spacer structures in the detected CRISPR cassette are the Staphylococcus, Mycobacterium, Streptococcus, Bacillus, Gordonia, Arthrobacter, Streptomyces. The implementation of the algorithm of program methods for locating CRISPR/Cas-loci can be applied to many other decoded bacterial genomes to return bacteriophage therapy.https://www.actabiomedica.ru/jour/article/view/706crispr/casсистема |
| spellingShingle | A. Yu. Borisenko Yu. P. Dzhioev N. P. Peretolchina L. A. Stepanenko V. A. Kuzminova Yu. M. Zemlyanskaya L. A. Kokorina N. A. Arefieva O. N. Reva Y. Wang Z. Qu V. I. Zlobin BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA Acta Biomedica Scientifica crispr/casсистема |
| title | BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA |
| title_full | BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA |
| title_fullStr | BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA |
| title_full_unstemmed | BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA |
| title_short | BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA |
| title_sort | bioinformation search and analysis of structures of crispr cas systems in phage staphylococus aureus genome and estimation of profiles of phage detected through crispr cassette bacteria |
| topic | crispr/casсистема |
| url | https://www.actabiomedica.ru/jour/article/view/706 |
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