Genome-Wide Identification and Characterization of MicroRNAs and Target Genes in Lonicera japonica.
MiRNAs function in post-transcriptional regulation of gene expression and play very important roles in plant development. Lonicera japonica is one of the important medicinal plants in China. However, few studies on the discovery of conserved and novel miRNAs from L. japonica were reported. In this s...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5053492?pdf=render |
| _version_ | 1818337648036544512 |
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| author | Heng Xia Libin Zhang Gang Wu Chunhua Fu Yan Long Jun Xiang Jianping Gan Yanhong Zhou Longjiang Yu Maoteng Li |
| author_facet | Heng Xia Libin Zhang Gang Wu Chunhua Fu Yan Long Jun Xiang Jianping Gan Yanhong Zhou Longjiang Yu Maoteng Li |
| author_sort | Heng Xia |
| collection | DOAJ |
| description | MiRNAs function in post-transcriptional regulation of gene expression and play very important roles in plant development. Lonicera japonica is one of the important medicinal plants in China. However, few studies on the discovery of conserved and novel miRNAs from L. japonica were reported. In this study, we employed deep sequencing technology to identify miRNAs in leaf and flower tissues of L. japonica. A total of 22.97 million clean reads from flower and leaf tissues were obtained, which generated 146 conserved miRNAs distributed in 20 families and 110 novel miRNAs. Accordingly, 72 differentially expressed miRNAs (P≤0.001) between leaves and flowers and their potential target genes were identified and validated. The qRT-PCR validation showed that majority of the differentially expressed miRNAs showed significant tissue-specific expression in L. japonica. Furthermore, the miRNA-mRNA and mRNA-mRNA regulatory networks were constructed using Cytoscape software. Taken together, this study identified a large number of miRNAs and target genes in L. japonica, which not only provides the first global miRNA expression profiles, but also sheds light on functional genomics research on L. japonica in the future. |
| first_indexed | 2024-12-13T14:58:33Z |
| format | Article |
| id | doaj.art-717403c88cf54506bb08463387b344d2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T14:58:33Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-717403c88cf54506bb08463387b344d22022-12-21T23:41:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011110e016414010.1371/journal.pone.0164140Genome-Wide Identification and Characterization of MicroRNAs and Target Genes in Lonicera japonica.Heng XiaLibin ZhangGang WuChunhua FuYan LongJun XiangJianping GanYanhong ZhouLongjiang YuMaoteng LiMiRNAs function in post-transcriptional regulation of gene expression and play very important roles in plant development. Lonicera japonica is one of the important medicinal plants in China. However, few studies on the discovery of conserved and novel miRNAs from L. japonica were reported. In this study, we employed deep sequencing technology to identify miRNAs in leaf and flower tissues of L. japonica. A total of 22.97 million clean reads from flower and leaf tissues were obtained, which generated 146 conserved miRNAs distributed in 20 families and 110 novel miRNAs. Accordingly, 72 differentially expressed miRNAs (P≤0.001) between leaves and flowers and their potential target genes were identified and validated. The qRT-PCR validation showed that majority of the differentially expressed miRNAs showed significant tissue-specific expression in L. japonica. Furthermore, the miRNA-mRNA and mRNA-mRNA regulatory networks were constructed using Cytoscape software. Taken together, this study identified a large number of miRNAs and target genes in L. japonica, which not only provides the first global miRNA expression profiles, but also sheds light on functional genomics research on L. japonica in the future.http://europepmc.org/articles/PMC5053492?pdf=render |
| spellingShingle | Heng Xia Libin Zhang Gang Wu Chunhua Fu Yan Long Jun Xiang Jianping Gan Yanhong Zhou Longjiang Yu Maoteng Li Genome-Wide Identification and Characterization of MicroRNAs and Target Genes in Lonicera japonica. PLoS ONE |
| title | Genome-Wide Identification and Characterization of MicroRNAs and Target Genes in Lonicera japonica. |
| title_full | Genome-Wide Identification and Characterization of MicroRNAs and Target Genes in Lonicera japonica. |
| title_fullStr | Genome-Wide Identification and Characterization of MicroRNAs and Target Genes in Lonicera japonica. |
| title_full_unstemmed | Genome-Wide Identification and Characterization of MicroRNAs and Target Genes in Lonicera japonica. |
| title_short | Genome-Wide Identification and Characterization of MicroRNAs and Target Genes in Lonicera japonica. |
| title_sort | genome wide identification and characterization of micrornas and target genes in lonicera japonica |
| url | http://europepmc.org/articles/PMC5053492?pdf=render |
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