Dietary carbon loaded with nano-ZnO alters the gut microbiota community to mediate bile acid metabolism and potentiate intestinal immune function in fattening beef cattle
Abstract Background To our knowledge, carbon loaded with nano-ZnO (NZnOC) represents a new nutritional additive for the animal husbandry industry. However, the mechanism by which NZnOC mediates beef cattle growth and intestinal health is not fully understood. This study aimed to investigate the effe...
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BMC
2022-12-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03483-2 |
| _version_ | 1811203771891449856 |
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| author | Haibo Zhang Weikun Guan Lizhi Li Dongsheng Guo Xiangfei Zhang Jiuqiang Guan Runxiao Luo Siying Zheng Jiangwen Fu Yingying Cheng Qin He |
| author_facet | Haibo Zhang Weikun Guan Lizhi Li Dongsheng Guo Xiangfei Zhang Jiuqiang Guan Runxiao Luo Siying Zheng Jiangwen Fu Yingying Cheng Qin He |
| author_sort | Haibo Zhang |
| collection | DOAJ |
| description | Abstract Background To our knowledge, carbon loaded with nano-ZnO (NZnOC) represents a new nutritional additive for the animal husbandry industry. However, the mechanism by which NZnOC mediates beef cattle growth and intestinal health is not fully understood. This study aimed to investigate the effects of carbon loaded with nano-ZnO (NZnOC) supplementation on growth performance, gut microbiota, bile acid (BAs) metabolism and intestinal immunity in fattening cattle. Twenty cattle (16 ± 0.95 months) were randomly assigned to two dietary groups: CON (control, without feed additive) and NZnOC (diet supplemented with 80 mg NZnOC/kg diet dry matter basic) for 60 d. The colon digesta microbiota composition and BAs concentration were determined by microbiota metagenomics and gas chromatography methods, respectively. Results The results showed that the NZnOC-supplemented cattle had greater final weight, average daily gain and gain-to-feed ratio than those in the CON group. Cattle fed the NZnOC diet had a higher relative abundance of the secondary BAs synthesizing phyla Firmicutes, Tenericutes and Actinobacteria than those fed the CON diet. Dietary supplementation with NZnOC increased the relative abundance of the secondary BAs synthesis microbiota genera Clostridium, Ruminococcus, Eubacterium, and Brevibacillus in colon digesta. Cattle fed the NZnOC diet had increased activities of 3α-hydroxysteroid dehydrogenase (EC: 1.1.1.52) and bile acid-CoA ligase BaiB (EC: 6.2.1.7) in the colon digesta compared with those fed the CON diet. The primary BAs taurocholic acid, taurochenodeoxycholic acid and taurodeoxycholate acid were significantly decreased by dietary NZnOC supplementation, while the secondary BAs deoxycholic acid, taurolithocholic acid, beta-muricholic acid, 12-ketolithocholic acid and ursodeoxycholic acid were significantly increased. Dietary supplementation with NZnOC increased the mRNA abundance of G protein-coupled bile acid receptor 1, protein kinase cAMP-activated catalytic subunit alpha, cyclic-AMP response element binding protein 1 and interleukin (IL)-10 in the colon mucosa of cattle, while the mRNA abundance of tumor necrosis factor and IL-1β were significantly decreased. Conclusions In summary, dietary supplementation with NZnOC can facilitate the growth performance and intestinal immune function of cattle by improving BAs metabolism. NZnOC can be supplemented in the diet as a safe regulator of gut microbiota and as a feed additive in the ruminants industry. |
| first_indexed | 2024-04-12T03:00:45Z |
| format | Article |
| id | doaj.art-7179d27bf4a649c2b8b9fcb263a64e00 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-12T03:00:45Z |
| publishDate | 2022-12-01 |
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| series | BMC Veterinary Research |
| spelling | doaj.art-7179d27bf4a649c2b8b9fcb263a64e002022-12-22T03:50:41ZengBMCBMC Veterinary Research1746-61482022-12-0118111610.1186/s12917-022-03483-2Dietary carbon loaded with nano-ZnO alters the gut microbiota community to mediate bile acid metabolism and potentiate intestinal immune function in fattening beef cattleHaibo Zhang0Weikun Guan1Lizhi Li2Dongsheng Guo3Xiangfei Zhang4Jiuqiang Guan5Runxiao Luo6Siying Zheng7Jiangwen Fu8Yingying Cheng9Qin He10Institute of College of Life Science and Resources and Environment, Yichun UniversityInstitute of College of Life Science and Resources and Environment, Yichun UniversityInstitute of College of Life Science and Resources and Environment, Yichun UniversityInstitute of College of Life Science and Resources and Environment, Yichun UniversitySichuan Academy of Grassland SciencesSichuan Academy of Grassland SciencesInstitute of College of Life Science and Resources and Environment, Yichun UniversityInstitute of College of Life Science and Resources and Environment, Yichun UniversityInstitute of College of Life Science and Resources and Environment, Yichun UniversityInstitute of College of Life Science and Resources and Environment, Yichun UniversityCollege of Life Sciences, Nanchang Normal UniversityAbstract Background To our knowledge, carbon loaded with nano-ZnO (NZnOC) represents a new nutritional additive for the animal husbandry industry. However, the mechanism by which NZnOC mediates beef cattle growth and intestinal health is not fully understood. This study aimed to investigate the effects of carbon loaded with nano-ZnO (NZnOC) supplementation on growth performance, gut microbiota, bile acid (BAs) metabolism and intestinal immunity in fattening cattle. Twenty cattle (16 ± 0.95 months) were randomly assigned to two dietary groups: CON (control, without feed additive) and NZnOC (diet supplemented with 80 mg NZnOC/kg diet dry matter basic) for 60 d. The colon digesta microbiota composition and BAs concentration were determined by microbiota metagenomics and gas chromatography methods, respectively. Results The results showed that the NZnOC-supplemented cattle had greater final weight, average daily gain and gain-to-feed ratio than those in the CON group. Cattle fed the NZnOC diet had a higher relative abundance of the secondary BAs synthesizing phyla Firmicutes, Tenericutes and Actinobacteria than those fed the CON diet. Dietary supplementation with NZnOC increased the relative abundance of the secondary BAs synthesis microbiota genera Clostridium, Ruminococcus, Eubacterium, and Brevibacillus in colon digesta. Cattle fed the NZnOC diet had increased activities of 3α-hydroxysteroid dehydrogenase (EC: 1.1.1.52) and bile acid-CoA ligase BaiB (EC: 6.2.1.7) in the colon digesta compared with those fed the CON diet. The primary BAs taurocholic acid, taurochenodeoxycholic acid and taurodeoxycholate acid were significantly decreased by dietary NZnOC supplementation, while the secondary BAs deoxycholic acid, taurolithocholic acid, beta-muricholic acid, 12-ketolithocholic acid and ursodeoxycholic acid were significantly increased. Dietary supplementation with NZnOC increased the mRNA abundance of G protein-coupled bile acid receptor 1, protein kinase cAMP-activated catalytic subunit alpha, cyclic-AMP response element binding protein 1 and interleukin (IL)-10 in the colon mucosa of cattle, while the mRNA abundance of tumor necrosis factor and IL-1β were significantly decreased. Conclusions In summary, dietary supplementation with NZnOC can facilitate the growth performance and intestinal immune function of cattle by improving BAs metabolism. NZnOC can be supplemented in the diet as a safe regulator of gut microbiota and as a feed additive in the ruminants industry.https://doi.org/10.1186/s12917-022-03483-2CattleCarbon loaded with nano-ZnOGut microbiotaBile acid metabolismIntestinal immune function |
| spellingShingle | Haibo Zhang Weikun Guan Lizhi Li Dongsheng Guo Xiangfei Zhang Jiuqiang Guan Runxiao Luo Siying Zheng Jiangwen Fu Yingying Cheng Qin He Dietary carbon loaded with nano-ZnO alters the gut microbiota community to mediate bile acid metabolism and potentiate intestinal immune function in fattening beef cattle BMC Veterinary Research Cattle Carbon loaded with nano-ZnO Gut microbiota Bile acid metabolism Intestinal immune function |
| title | Dietary carbon loaded with nano-ZnO alters the gut microbiota community to mediate bile acid metabolism and potentiate intestinal immune function in fattening beef cattle |
| title_full | Dietary carbon loaded with nano-ZnO alters the gut microbiota community to mediate bile acid metabolism and potentiate intestinal immune function in fattening beef cattle |
| title_fullStr | Dietary carbon loaded with nano-ZnO alters the gut microbiota community to mediate bile acid metabolism and potentiate intestinal immune function in fattening beef cattle |
| title_full_unstemmed | Dietary carbon loaded with nano-ZnO alters the gut microbiota community to mediate bile acid metabolism and potentiate intestinal immune function in fattening beef cattle |
| title_short | Dietary carbon loaded with nano-ZnO alters the gut microbiota community to mediate bile acid metabolism and potentiate intestinal immune function in fattening beef cattle |
| title_sort | dietary carbon loaded with nano zno alters the gut microbiota community to mediate bile acid metabolism and potentiate intestinal immune function in fattening beef cattle |
| topic | Cattle Carbon loaded with nano-ZnO Gut microbiota Bile acid metabolism Intestinal immune function |
| url | https://doi.org/10.1186/s12917-022-03483-2 |
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