Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves
Abstract The global spread of critical-priority antimicrobial-resistant Enterobacterales by food is a public health problem. Wild-caught seafood are broadly consumed worldwide, but exposure to land-based pollution can favor their contamination by clinically relevant antimicrobial-resistant bacteria....
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| Format: | Article |
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Nature Portfolio
2022-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-09598-8 |
| _version_ | 1819209995896487936 |
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| author | Vanessa Bueris Fábio P. Sellera Bruna Fuga Elder Sano Marcelo P. N. Carvalho Samuel C. F. Couto Quézia Moura Nilton Lincopan |
| author_facet | Vanessa Bueris Fábio P. Sellera Bruna Fuga Elder Sano Marcelo P. N. Carvalho Samuel C. F. Couto Quézia Moura Nilton Lincopan |
| author_sort | Vanessa Bueris |
| collection | DOAJ |
| description | Abstract The global spread of critical-priority antimicrobial-resistant Enterobacterales by food is a public health problem. Wild-caught seafood are broadly consumed worldwide, but exposure to land-based pollution can favor their contamination by clinically relevant antimicrobial-resistant bacteria. As part of the Grand Challenges Explorations: New Approaches to Characterize the Global Burden of Antimicrobial Resistance Program, we performed genomic surveillance and cell culture-based virulence investigation of WHO critical priority Enterobacterales isolated from marine bivalves collected in the Atlantic Coast of South America. Broad-spectrum cephalosporin-resistant Klebsiella pneumoniae and Escherichia coli isolates were recovered from eight distinct geographical locations. These strains harbored bla CTX-M-type or bla CMY-type genes. Most of the surveyed genomes confirmed the convergence of wide virulome and resistome (i.e., antimicrobials, heavy metals, biocides, and pesticides resistance). We identified strains belonging to the international high-risk clones K. pneumoniae ST307 and E. coli ST131 carrying important virulence genes, whereas in vitro experiments confirmed the high virulence potential of these strains. Thermolabile and thermostable toxins were identified in some strains, and all of them were biofilm producers. These data point to an alarming presence of resistance and virulence genes in marine environments, which may favor horizontal gene transfer and the spread of these traits to other bacterial species. |
| first_indexed | 2024-12-23T06:04:09Z |
| format | Article |
| id | doaj.art-717ef3f969db425d95e3735e72a2b430 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T06:04:09Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-717ef3f969db425d95e3735e72a2b4302022-12-21T17:57:36ZengNature PortfolioScientific Reports2045-23222022-04-0112111210.1038/s41598-022-09598-8Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine BivalvesVanessa Bueris0Fábio P. Sellera1Bruna Fuga2Elder Sano3Marcelo P. N. Carvalho4Samuel C. F. Couto5Quézia Moura6Nilton Lincopan7Department of Microbiology, Institute of Biomedical Sciences, University of São PauloDepartment of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São PauloDepartment of Microbiology, Institute of Biomedical Sciences, University of São PauloDepartment of Microbiology, Institute of Biomedical Sciences, University of São PauloDepartment of Veterinary Clinic and Surgery, Federal University of Minas GeraisLaboratory of Genetics, Butantan InstituteDepartment of Microbiology, Institute of Biomedical Sciences, University of São PauloDepartment of Microbiology, Institute of Biomedical Sciences, University of São PauloAbstract The global spread of critical-priority antimicrobial-resistant Enterobacterales by food is a public health problem. Wild-caught seafood are broadly consumed worldwide, but exposure to land-based pollution can favor their contamination by clinically relevant antimicrobial-resistant bacteria. As part of the Grand Challenges Explorations: New Approaches to Characterize the Global Burden of Antimicrobial Resistance Program, we performed genomic surveillance and cell culture-based virulence investigation of WHO critical priority Enterobacterales isolated from marine bivalves collected in the Atlantic Coast of South America. Broad-spectrum cephalosporin-resistant Klebsiella pneumoniae and Escherichia coli isolates were recovered from eight distinct geographical locations. These strains harbored bla CTX-M-type or bla CMY-type genes. Most of the surveyed genomes confirmed the convergence of wide virulome and resistome (i.e., antimicrobials, heavy metals, biocides, and pesticides resistance). We identified strains belonging to the international high-risk clones K. pneumoniae ST307 and E. coli ST131 carrying important virulence genes, whereas in vitro experiments confirmed the high virulence potential of these strains. Thermolabile and thermostable toxins were identified in some strains, and all of them were biofilm producers. These data point to an alarming presence of resistance and virulence genes in marine environments, which may favor horizontal gene transfer and the spread of these traits to other bacterial species.https://doi.org/10.1038/s41598-022-09598-8 |
| spellingShingle | Vanessa Bueris Fábio P. Sellera Bruna Fuga Elder Sano Marcelo P. N. Carvalho Samuel C. F. Couto Quézia Moura Nilton Lincopan Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves Scientific Reports |
| title | Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
| title_full | Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
| title_fullStr | Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
| title_full_unstemmed | Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
| title_short | Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves |
| title_sort | convergence of virulence and resistance in international clones of who critical priority enterobacterales isolated from marine bivalves |
| url | https://doi.org/10.1038/s41598-022-09598-8 |
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