Metal Tolerance Protein Encoding Gene Family in <i>Fagopyrum tartaricum</i>: Genome-Wide Identification, Characterization and Expression under Multiple Metal Stresses
Metal tolerance proteins (MTP) as divalent cation transporters are essential for plant metal tolerance and homeostasis. However, the characterization and the definitive phylogeny of the MTP gene family in <i>Fagopyrum tartaricum</i>, and their roles in response to metal stress are still...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/7/850 |
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| author | Zhiqiang Li Chenglong Wang Kaiyi Wang Jiayu Zhao Jirong Shao Hui Chen Meiliang Zhou Xuemei Zhu |
| author_facet | Zhiqiang Li Chenglong Wang Kaiyi Wang Jiayu Zhao Jirong Shao Hui Chen Meiliang Zhou Xuemei Zhu |
| author_sort | Zhiqiang Li |
| collection | DOAJ |
| description | Metal tolerance proteins (MTP) as divalent cation transporters are essential for plant metal tolerance and homeostasis. However, the characterization and the definitive phylogeny of the MTP gene family in <i>Fagopyrum tartaricum</i>, and their roles in response to metal stress are still unknown. In the present study, MTP genes in <i>Fagopyrum tartaricum</i> were identified, and their phylogenetic relationships, structural characteristics, physicochemical parameters, as well as expression profiles under five metal stresses including Fe, Mn, Cu, Zn, and Cd were also investigated. Phylogenetic relationship analysis showed that 12 <i>Fagopyrum tartaricum</i> MTP genes were classified into three major clusters and seven groups. All <i>FtMTP</i>s had typical structural features of the MTP gene family and were predicted to be located in the cell vacuole. The upstream region of <i>FtMTP</i>s contained abundant cis-acting elements, implying their functions in development progress and stress response. Tissue-specific expression analysis results indicated the regulation of <i>FtMTP</i>s in the growth and development of <i>Fagopyrum tataricum</i>. Besides, the expression of most <i>FtMTP</i> genes could be induced by multiple metals and showed different expression patterns under at least two metal stresses. These findings provide useful information for the research of the metal tolerance mechanism and genetic improvement of <i>Fagopyrum tataricum</i>. |
| first_indexed | 2024-03-09T11:31:36Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:31:36Z |
| publishDate | 2022-03-01 |
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| series | Plants |
| spelling | doaj.art-71801e4bdf354dc091b587224f3e06442023-11-30T23:49:50ZengMDPI AGPlants2223-77472022-03-0111785010.3390/plants11070850Metal Tolerance Protein Encoding Gene Family in <i>Fagopyrum tartaricum</i>: Genome-Wide Identification, Characterization and Expression under Multiple Metal StressesZhiqiang Li0Chenglong Wang1Kaiyi Wang2Jiayu Zhao3Jirong Shao4Hui Chen5Meiliang Zhou6Xuemei Zhu7College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, ChinaSchool of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaCollege of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, ChinaMetal tolerance proteins (MTP) as divalent cation transporters are essential for plant metal tolerance and homeostasis. However, the characterization and the definitive phylogeny of the MTP gene family in <i>Fagopyrum tartaricum</i>, and their roles in response to metal stress are still unknown. In the present study, MTP genes in <i>Fagopyrum tartaricum</i> were identified, and their phylogenetic relationships, structural characteristics, physicochemical parameters, as well as expression profiles under five metal stresses including Fe, Mn, Cu, Zn, and Cd were also investigated. Phylogenetic relationship analysis showed that 12 <i>Fagopyrum tartaricum</i> MTP genes were classified into three major clusters and seven groups. All <i>FtMTP</i>s had typical structural features of the MTP gene family and were predicted to be located in the cell vacuole. The upstream region of <i>FtMTP</i>s contained abundant cis-acting elements, implying their functions in development progress and stress response. Tissue-specific expression analysis results indicated the regulation of <i>FtMTP</i>s in the growth and development of <i>Fagopyrum tataricum</i>. Besides, the expression of most <i>FtMTP</i> genes could be induced by multiple metals and showed different expression patterns under at least two metal stresses. These findings provide useful information for the research of the metal tolerance mechanism and genetic improvement of <i>Fagopyrum tataricum</i>.https://www.mdpi.com/2223-7747/11/7/850<i>Fagopyrum tataricum</i>gene expressionmetal stressmetal tolerance protein |
| spellingShingle | Zhiqiang Li Chenglong Wang Kaiyi Wang Jiayu Zhao Jirong Shao Hui Chen Meiliang Zhou Xuemei Zhu Metal Tolerance Protein Encoding Gene Family in <i>Fagopyrum tartaricum</i>: Genome-Wide Identification, Characterization and Expression under Multiple Metal Stresses Plants <i>Fagopyrum tataricum</i> gene expression metal stress metal tolerance protein |
| title | Metal Tolerance Protein Encoding Gene Family in <i>Fagopyrum tartaricum</i>: Genome-Wide Identification, Characterization and Expression under Multiple Metal Stresses |
| title_full | Metal Tolerance Protein Encoding Gene Family in <i>Fagopyrum tartaricum</i>: Genome-Wide Identification, Characterization and Expression under Multiple Metal Stresses |
| title_fullStr | Metal Tolerance Protein Encoding Gene Family in <i>Fagopyrum tartaricum</i>: Genome-Wide Identification, Characterization and Expression under Multiple Metal Stresses |
| title_full_unstemmed | Metal Tolerance Protein Encoding Gene Family in <i>Fagopyrum tartaricum</i>: Genome-Wide Identification, Characterization and Expression under Multiple Metal Stresses |
| title_short | Metal Tolerance Protein Encoding Gene Family in <i>Fagopyrum tartaricum</i>: Genome-Wide Identification, Characterization and Expression under Multiple Metal Stresses |
| title_sort | metal tolerance protein encoding gene family in i fagopyrum tartaricum i genome wide identification characterization and expression under multiple metal stresses |
| topic | <i>Fagopyrum tataricum</i> gene expression metal stress metal tolerance protein |
| url | https://www.mdpi.com/2223-7747/11/7/850 |
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