A new biosensing platform based on L-cysteine-capped Fe3O4 nanoparticles embedded in chitosan-MWCNT matrix: Electrochemical kinetic and sensing studies
We present a report on the electrochemical kinetics and sensing studies conducted on a flexible ITO-PET transducer, fabricated with a new nanocomposite of L-cysteine coated iron oxide nanoparticles (LCys@Fe3O4 NPs) and multi-wall carbon nanotubes (MWCNT), for the first time. Synthesized via the wet-...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137023001097 |
| _version_ | 1797398500143529984 |
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| author | Tamal Sarkar Nirmita Dutta Gorachand Dutta |
| author_facet | Tamal Sarkar Nirmita Dutta Gorachand Dutta |
| author_sort | Tamal Sarkar |
| collection | DOAJ |
| description | We present a report on the electrochemical kinetics and sensing studies conducted on a flexible ITO-PET transducer, fabricated with a new nanocomposite of L-cysteine coated iron oxide nanoparticles (LCys@Fe3O4 NPs) and multi-wall carbon nanotubes (MWCNT), for the first time. Synthesized via the wet-chemical co-precipitation method, the LCys@Fe3O4 NPs exhibit a pure cubic phase. A nanocomposite of these LCys@Fe3O4 NPs are prepared via dispersion in chitosan (CS) solution along with MWCNT, and further drop-casted onto a low-cost, flexible ITO-PET sheet. The fabricated CS-MWCNT-LCys@Fe3O4/ITO-PET electrode shows a higher surface roughness, compared to either MWCNT or LCys@Fe3O4 modified electrode counterpart. The CS-MWCNT-LCys@Fe3O4 nanocomposite exhibits a twofold enhancement of the peak anodic voltammetric current, and a tenfold decrease in the charge transfer resistance, as compared to the LCys@Fe3O4 nanoparticles. Additionally, a mathematical model is devised to evaluate the kinetic parameters (ks, α & DFe(II)) of the redox species K4[Fe(CN)6]/K3[Fe(CN)6] on the CS-MWCNT-LCys@Fe3O4/ITO-PET surface, confirming a quasi-reversible electron transfer process. We couple this transducer with an immunosandwich format to facilitate rapid and ultrasensitive electrochemical measurement of protein biomarkers. Proof-of-concept is carried out by measuring the SARS-CoV-2 spike glycoprotein on the biofunctionalized Ab1/CS-MWCNT-LCys@Fe3O4/ITO-PET immunoelectrodes within a wide linear range (1 pg mL-1-1 μg mL-1). The immunoelectrode encompasses a limit of detection of 0.932 pg mL-1, and a sensitivity of 0.499 μA pg−1 mL cm−2. Thus, CS-MWCNT-LCys@Fe3O4 presents a new and excellent nanocomposite for biosensing application, while the Ab1/CS-MWCNT-LCys@Fe3O4/ITO-PET immunoelectrode can serve as an efficient, flexible, and cost-effective biosensing platform which can further be utilised to fabricate point-of-care biosensors. |
| first_indexed | 2024-03-09T01:26:24Z |
| format | Article |
| id | doaj.art-7182443df09c4e1c8f6eb7490de851af |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-03-09T01:26:24Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-7182443df09c4e1c8f6eb7490de851af2023-12-10T06:17:27ZengElsevierBiosensors and Bioelectronics: X2590-13702023-12-0115100412A new biosensing platform based on L-cysteine-capped Fe3O4 nanoparticles embedded in chitosan-MWCNT matrix: Electrochemical kinetic and sensing studiesTamal Sarkar0Nirmita Dutta1Gorachand Dutta2NanoBiosensors and Biodevices Lab, School of Medical Sciences and Technology, Indian Institute of Technology, Kharagpur, 721302, IndiaNanoBiosensors and Biodevices Lab, School of Medical Sciences and Technology, Indian Institute of Technology, Kharagpur, 721302, IndiaCorresponding author.; NanoBiosensors and Biodevices Lab, School of Medical Sciences and Technology, Indian Institute of Technology, Kharagpur, 721302, IndiaWe present a report on the electrochemical kinetics and sensing studies conducted on a flexible ITO-PET transducer, fabricated with a new nanocomposite of L-cysteine coated iron oxide nanoparticles (LCys@Fe3O4 NPs) and multi-wall carbon nanotubes (MWCNT), for the first time. Synthesized via the wet-chemical co-precipitation method, the LCys@Fe3O4 NPs exhibit a pure cubic phase. A nanocomposite of these LCys@Fe3O4 NPs are prepared via dispersion in chitosan (CS) solution along with MWCNT, and further drop-casted onto a low-cost, flexible ITO-PET sheet. The fabricated CS-MWCNT-LCys@Fe3O4/ITO-PET electrode shows a higher surface roughness, compared to either MWCNT or LCys@Fe3O4 modified electrode counterpart. The CS-MWCNT-LCys@Fe3O4 nanocomposite exhibits a twofold enhancement of the peak anodic voltammetric current, and a tenfold decrease in the charge transfer resistance, as compared to the LCys@Fe3O4 nanoparticles. Additionally, a mathematical model is devised to evaluate the kinetic parameters (ks, α & DFe(II)) of the redox species K4[Fe(CN)6]/K3[Fe(CN)6] on the CS-MWCNT-LCys@Fe3O4/ITO-PET surface, confirming a quasi-reversible electron transfer process. We couple this transducer with an immunosandwich format to facilitate rapid and ultrasensitive electrochemical measurement of protein biomarkers. Proof-of-concept is carried out by measuring the SARS-CoV-2 spike glycoprotein on the biofunctionalized Ab1/CS-MWCNT-LCys@Fe3O4/ITO-PET immunoelectrodes within a wide linear range (1 pg mL-1-1 μg mL-1). The immunoelectrode encompasses a limit of detection of 0.932 pg mL-1, and a sensitivity of 0.499 μA pg−1 mL cm−2. Thus, CS-MWCNT-LCys@Fe3O4 presents a new and excellent nanocomposite for biosensing application, while the Ab1/CS-MWCNT-LCys@Fe3O4/ITO-PET immunoelectrode can serve as an efficient, flexible, and cost-effective biosensing platform which can further be utilised to fabricate point-of-care biosensors.http://www.sciencedirect.com/science/article/pii/S2590137023001097Iron oxide nanoparticlesL-cysteineCarbon nanotubeITO-PETElectrochemical biosensor |
| spellingShingle | Tamal Sarkar Nirmita Dutta Gorachand Dutta A new biosensing platform based on L-cysteine-capped Fe3O4 nanoparticles embedded in chitosan-MWCNT matrix: Electrochemical kinetic and sensing studies Biosensors and Bioelectronics: X Iron oxide nanoparticles L-cysteine Carbon nanotube ITO-PET Electrochemical biosensor |
| title | A new biosensing platform based on L-cysteine-capped Fe3O4 nanoparticles embedded in chitosan-MWCNT matrix: Electrochemical kinetic and sensing studies |
| title_full | A new biosensing platform based on L-cysteine-capped Fe3O4 nanoparticles embedded in chitosan-MWCNT matrix: Electrochemical kinetic and sensing studies |
| title_fullStr | A new biosensing platform based on L-cysteine-capped Fe3O4 nanoparticles embedded in chitosan-MWCNT matrix: Electrochemical kinetic and sensing studies |
| title_full_unstemmed | A new biosensing platform based on L-cysteine-capped Fe3O4 nanoparticles embedded in chitosan-MWCNT matrix: Electrochemical kinetic and sensing studies |
| title_short | A new biosensing platform based on L-cysteine-capped Fe3O4 nanoparticles embedded in chitosan-MWCNT matrix: Electrochemical kinetic and sensing studies |
| title_sort | new biosensing platform based on l cysteine capped fe3o4 nanoparticles embedded in chitosan mwcnt matrix electrochemical kinetic and sensing studies |
| topic | Iron oxide nanoparticles L-cysteine Carbon nanotube ITO-PET Electrochemical biosensor |
| url | http://www.sciencedirect.com/science/article/pii/S2590137023001097 |
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