Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J mice
Purpose: Many diet based studies on metabolic syndrome lack an in-depth study of feed nutrient profile post preparation. We analyzed the nutritional profile of formulated High Fat Simple Carbohydrate (HFSC) feed and studied its effect on target organs. Methods: Control feed and HFSC feed were analyz...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Journal of Nutrition & Intermediary Metabolism |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352385915300347 |
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| author | Serena Stephen D'Souza B. Sri Charan Bindu M. Mohammed Ali Alex Tisha K.V. Deepthi Silveira Siona Fernandes Santy Asha Abraham |
| author_facet | Serena Stephen D'Souza B. Sri Charan Bindu M. Mohammed Ali Alex Tisha K.V. Deepthi Silveira Siona Fernandes Santy Asha Abraham |
| author_sort | Serena Stephen D'Souza |
| collection | DOAJ |
| description | Purpose: Many diet based studies on metabolic syndrome lack an in-depth study of feed nutrient profile post preparation. We analyzed the nutritional profile of formulated High Fat Simple Carbohydrate (HFSC) feed and studied its effect on target organs.
Methods: Control feed and HFSC feed were analyzed for macro and micronutrient profile and fed to one month male C57BL/6J mice for 5 months during which feed intake, energy intake and feed efficiency was monitored. The effects of feeds were preliminarily studied on kidney, liver, adrenal gland and pancreas by histological staining.
Results: The HFSC feed had significantly lower carbohydrate, moisture, crude fiber, ash content, mineral, vitamin content, higher ω-6/ω-3 fatty acid ratio as compared to control feed. The HFSC fed mice had higher food intake; energy intake; feed efficiency; lymphocyte infiltration in the liver; hypertrophy of the kidney, adrenal medulla and degenerated islets of pancreas as compared to the control mice after 5 months of feeding.
Conclusion: Food with a higher ω-6/ω-3 fatty acid ratio and simple carbohydrate leads to deterioration of structural integrity of vital organs. |
| first_indexed | 2024-12-11T06:03:19Z |
| format | Article |
| id | doaj.art-7183ba5ed8904fdbba972c0b86f012b1 |
| institution | Directory Open Access Journal |
| issn | 2352-3859 |
| language | English |
| last_indexed | 2024-12-11T06:03:19Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Nutrition & Intermediary Metabolism |
| spelling | doaj.art-7183ba5ed8904fdbba972c0b86f012b12022-12-22T01:18:23ZengElsevierJournal of Nutrition & Intermediary Metabolism2352-38592016-03-013C414910.1016/j.jnim.2015.12.334Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J miceSerena Stephen D'Souza0B. Sri Charan Bindu1M. Mohammed Ali2Alex Tisha3K.V. Deepthi4Silveira Siona5Fernandes Santy6Asha Abraham7Father George Albuquerque Pai Cell and Molecular Biology Laboratory, Department of Postgraduate Studies and Research in Biotechnology, St Aloysius College, Mangalore – 575003, IndiaDepartment of Meat, Fish and Poultry Technology, Central Food Technological Research Institute, Mysore 570 020 IndiaDepartment of Meat, Fish and Poultry Technology, Central Food Technological Research Institute, Mysore 570 020 IndiaFather George Albuquerque Pai Cell and Molecular Biology Laboratory, Department of Postgraduate Studies and Research in Biotechnology, St Aloysius College, Mangalore – 575003, IndiaFather George Albuquerque Pai Cell and Molecular Biology Laboratory, Department of Postgraduate Studies and Research in Biotechnology, St Aloysius College, Mangalore – 575003, IndiaFather George Albuquerque Pai Cell and Molecular Biology Laboratory, Department of Postgraduate Studies and Research in Biotechnology, St Aloysius College, Mangalore – 575003, IndiaFather George Albuquerque Pai Cell and Molecular Biology Laboratory, Department of Postgraduate Studies and Research in Biotechnology, St Aloysius College, Mangalore – 575003, IndiaFather George Albuquerque Pai Cell and Molecular Biology Laboratory, Department of Postgraduate Studies and Research in Biotechnology, St Aloysius College, Mangalore – 575003, IndiaPurpose: Many diet based studies on metabolic syndrome lack an in-depth study of feed nutrient profile post preparation. We analyzed the nutritional profile of formulated High Fat Simple Carbohydrate (HFSC) feed and studied its effect on target organs. Methods: Control feed and HFSC feed were analyzed for macro and micronutrient profile and fed to one month male C57BL/6J mice for 5 months during which feed intake, energy intake and feed efficiency was monitored. The effects of feeds were preliminarily studied on kidney, liver, adrenal gland and pancreas by histological staining. Results: The HFSC feed had significantly lower carbohydrate, moisture, crude fiber, ash content, mineral, vitamin content, higher ω-6/ω-3 fatty acid ratio as compared to control feed. The HFSC fed mice had higher food intake; energy intake; feed efficiency; lymphocyte infiltration in the liver; hypertrophy of the kidney, adrenal medulla and degenerated islets of pancreas as compared to the control mice after 5 months of feeding. Conclusion: Food with a higher ω-6/ω-3 fatty acid ratio and simple carbohydrate leads to deterioration of structural integrity of vital organs.http://www.sciencedirect.com/science/article/pii/S2352385915300347C57BL/6JFatty acid profileHigh fat simple carbohydrate feedω-6/ω-3Fatty liverIslet hypertrophy |
| spellingShingle | Serena Stephen D'Souza B. Sri Charan Bindu M. Mohammed Ali Alex Tisha K.V. Deepthi Silveira Siona Fernandes Santy Asha Abraham Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J mice Journal of Nutrition & Intermediary Metabolism C57BL/6J Fatty acid profile High fat simple carbohydrate feed ω-6/ω-3 Fatty liver Islet hypertrophy |
| title | Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J mice |
| title_full | Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J mice |
| title_fullStr | Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J mice |
| title_full_unstemmed | Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J mice |
| title_short | Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J mice |
| title_sort | nutritional profile of high fat simple carbohydrate diet used to induce metabolic syndrome in c57bl 6j mice |
| topic | C57BL/6J Fatty acid profile High fat simple carbohydrate feed ω-6/ω-3 Fatty liver Islet hypertrophy |
| url | http://www.sciencedirect.com/science/article/pii/S2352385915300347 |
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