Coconut Callus Initiation for Cell Suspension Culture
The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/4/968 |
| _version_ | 1797618516812103680 |
|---|---|
| author | Eveline Y. Y. Kong Julianne Biddle Sundaravelpandian Kalaipandian Steve W. Adkins |
| author_facet | Eveline Y. Y. Kong Julianne Biddle Sundaravelpandian Kalaipandian Steve W. Adkins |
| author_sort | Eveline Y. Y. Kong |
| collection | DOAJ |
| description | The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for developing cell suspension cultures. Hence, this study attempted to acquire friable EC through the following approaches: The manipulation of (1) medium type and subculture frequency, (2) a reduced 2,4-dichlorophenoxy acetic acid concentration during subculture, (3) the nitrate level and the ammonium-to-nitrate ratio, and the addition of amino acid mixture, (4) the addition of L-proline, and (5) the reduction of medium nutrients. Unfortunately, none of these culture conditions produced friable coconut EC. Even though friable EC was not achieved via these approaches, some of the conditions were found to influence the formation of compact EC, therefore these results are important for further studies focused on somatic embryogenesis in coconut and other species. |
| first_indexed | 2024-03-11T08:14:52Z |
| format | Article |
| id | doaj.art-7183bdea991a47e3a6feed918fcb814f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T08:14:52Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-7183bdea991a47e3a6feed918fcb814f2023-11-16T22:49:52ZengMDPI AGPlants2223-77472023-02-0112496810.3390/plants12040968Coconut Callus Initiation for Cell Suspension CultureEveline Y. Y. Kong0Julianne Biddle1Sundaravelpandian Kalaipandian2Steve W. Adkins3School of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, AustraliaSchool of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, AustraliaSchool of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, AustraliaSchool of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, AustraliaThe development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for developing cell suspension cultures. Hence, this study attempted to acquire friable EC through the following approaches: The manipulation of (1) medium type and subculture frequency, (2) a reduced 2,4-dichlorophenoxy acetic acid concentration during subculture, (3) the nitrate level and the ammonium-to-nitrate ratio, and the addition of amino acid mixture, (4) the addition of L-proline, and (5) the reduction of medium nutrients. Unfortunately, none of these culture conditions produced friable coconut EC. Even though friable EC was not achieved via these approaches, some of the conditions were found to influence the formation of compact EC, therefore these results are important for further studies focused on somatic embryogenesis in coconut and other species.https://www.mdpi.com/2223-7747/12/4/968calluscell suspension culturecoconut<i>Cocos nucifera</i> L.embryogenic callustissue culture |
| spellingShingle | Eveline Y. Y. Kong Julianne Biddle Sundaravelpandian Kalaipandian Steve W. Adkins Coconut Callus Initiation for Cell Suspension Culture Plants callus cell suspension culture coconut <i>Cocos nucifera</i> L. embryogenic callus tissue culture |
| title | Coconut Callus Initiation for Cell Suspension Culture |
| title_full | Coconut Callus Initiation for Cell Suspension Culture |
| title_fullStr | Coconut Callus Initiation for Cell Suspension Culture |
| title_full_unstemmed | Coconut Callus Initiation for Cell Suspension Culture |
| title_short | Coconut Callus Initiation for Cell Suspension Culture |
| title_sort | coconut callus initiation for cell suspension culture |
| topic | callus cell suspension culture coconut <i>Cocos nucifera</i> L. embryogenic callus tissue culture |
| url | https://www.mdpi.com/2223-7747/12/4/968 |
| work_keys_str_mv | AT evelineyykong coconutcallusinitiationforcellsuspensionculture AT juliannebiddle coconutcallusinitiationforcellsuspensionculture AT sundaravelpandiankalaipandian coconutcallusinitiationforcellsuspensionculture AT stevewadkins coconutcallusinitiationforcellsuspensionculture |