Study of four week repeated dose toxic test of Sweet Bee Venom in Beagle Dogs
Objectives: This study was performed to analyse four week repeated dose toxicity of Sweet Bee Venom(Sweet BV) extracted from the bee venom in Beagle dogs. Methods: All experiments were conducted under the regulations of Good Laboratory Practice (GLP) at Biotoxtech Company, a non-clinical study auth...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2010-12-01
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| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3831/KPI.2010.13.4.005 |
| _version_ | 1818452704554385408 |
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| author | Jae-Seuk Park Kwang-Ho Lee Ki-Rok Kwon |
| author_facet | Jae-Seuk Park Kwang-Ho Lee Ki-Rok Kwon |
| author_sort | Jae-Seuk Park |
| collection | DOAJ |
| description | Objectives: This study was performed to analyse four week repeated dose toxicity of Sweet Bee Venom(Sweet BV) extracted from the bee venom in Beagle dogs.
Methods: All experiments were conducted under the regulations of Good Laboratory Practice (GLP) at Biotoxtech Company, a non-clinical study authorized institution. Male and female Beagle dogs of 5-6 months old were chosen for the pilot study of four week repeated dose toxicity of Sweet BV which was administered at the level of 0.56㎎/㎏ body weight which is eighty times higher than the clinical application dosage as the high dosage, followed by 0.28 and 0.14㎎/㎏ as midium and low
dosage, respectively. Equal amount of excipient(normal saline) to the Sweet BV experiment groups was administered as the control group every day for four weeks.
Results: 1. No mortality was witnessed in all of the experiment groups.
2. All experiment groups were appealed pain sense in the treating time compared to the control group, and hyperemia and movement disorder were observed around the area of administration in all experiment groups, and higher occurrence in the higher dosage treatment.
3. For weight measurement, Neither male nor female groups showed significant changes.
4. In the urine analysis, CBC and biochemistry didn't show any significant changes in the experiment groups compared with control group.
5. For weight measurement of organs, experiment groups didn't show any significant changes compared with control group.
6. To verify abnormalities of organs and tissues, thigh muscle which treated with Sweet BV, cerebrum, liver, lung, kidney, and spinal cords were removed and conducted histologocal observation with H-E staining. In the histologocal observation of thigh muscle, cell infiltration, inflammatory, degeneration, necrosis of muscle fiber, and fibrosis were found in both thigh tissue. And the changes were depend on the dose of Sweet BV. But another organs were not detected in any abnormalities.
7. The proper high dosage of Sweet BV for the thirteen week repeated test in Beagle dogs may be 0.28㎎/㎏ in one time.
Conclusion: Above findings suggest that Sweet BV is relatively safe treatment medium. Further studies on the subject should be conducted to yield more concrete evidences. |
| first_indexed | 2024-12-14T21:27:19Z |
| format | Article |
| id | doaj.art-718491e766854135bfa8395928b75665 |
| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-12-14T21:27:19Z |
| publishDate | 2010-12-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-718491e766854135bfa8395928b756652022-12-21T22:46:46ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562010-12-0113454110.3831/KPI.2010.13.4.005Study of four week repeated dose toxic test of Sweet Bee Venom in Beagle DogsJae-Seuk Park0Kwang-Ho Lee1Ki-Rok Kwon2Dept. of Acupuncture & Moxibustion, Oriental Medical College, Sangji UniversityDept. of Acupuncture & Moxibustion, Oriental Medical College, Sangji UniversityDept. of Acupuncture & Moxibustion, Oriental Medical College, Sangji UniversityObjectives: This study was performed to analyse four week repeated dose toxicity of Sweet Bee Venom(Sweet BV) extracted from the bee venom in Beagle dogs. Methods: All experiments were conducted under the regulations of Good Laboratory Practice (GLP) at Biotoxtech Company, a non-clinical study authorized institution. Male and female Beagle dogs of 5-6 months old were chosen for the pilot study of four week repeated dose toxicity of Sweet BV which was administered at the level of 0.56㎎/㎏ body weight which is eighty times higher than the clinical application dosage as the high dosage, followed by 0.28 and 0.14㎎/㎏ as midium and low dosage, respectively. Equal amount of excipient(normal saline) to the Sweet BV experiment groups was administered as the control group every day for four weeks. Results: 1. No mortality was witnessed in all of the experiment groups. 2. All experiment groups were appealed pain sense in the treating time compared to the control group, and hyperemia and movement disorder were observed around the area of administration in all experiment groups, and higher occurrence in the higher dosage treatment. 3. For weight measurement, Neither male nor female groups showed significant changes. 4. In the urine analysis, CBC and biochemistry didn't show any significant changes in the experiment groups compared with control group. 5. For weight measurement of organs, experiment groups didn't show any significant changes compared with control group. 6. To verify abnormalities of organs and tissues, thigh muscle which treated with Sweet BV, cerebrum, liver, lung, kidney, and spinal cords were removed and conducted histologocal observation with H-E staining. In the histologocal observation of thigh muscle, cell infiltration, inflammatory, degeneration, necrosis of muscle fiber, and fibrosis were found in both thigh tissue. And the changes were depend on the dose of Sweet BV. But another organs were not detected in any abnormalities. 7. The proper high dosage of Sweet BV for the thirteen week repeated test in Beagle dogs may be 0.28㎎/㎏ in one time. Conclusion: Above findings suggest that Sweet BV is relatively safe treatment medium. Further studies on the subject should be conducted to yield more concrete evidences.http://dx.doi.org/10.3831/KPI.2010.13.4.005Sweet Bee Venommelittinfour week repeated dose toxicityBeagle dog |
| spellingShingle | Jae-Seuk Park Kwang-Ho Lee Ki-Rok Kwon Study of four week repeated dose toxic test of Sweet Bee Venom in Beagle Dogs Journal of Pharmacopuncture Sweet Bee Venom melittin four week repeated dose toxicity Beagle dog |
| title | Study of four week repeated dose toxic test of Sweet Bee Venom in Beagle Dogs |
| title_full | Study of four week repeated dose toxic test of Sweet Bee Venom in Beagle Dogs |
| title_fullStr | Study of four week repeated dose toxic test of Sweet Bee Venom in Beagle Dogs |
| title_full_unstemmed | Study of four week repeated dose toxic test of Sweet Bee Venom in Beagle Dogs |
| title_short | Study of four week repeated dose toxic test of Sweet Bee Venom in Beagle Dogs |
| title_sort | study of four week repeated dose toxic test of sweet bee venom in beagle dogs |
| topic | Sweet Bee Venom melittin four week repeated dose toxicity Beagle dog |
| url | http://dx.doi.org/10.3831/KPI.2010.13.4.005 |
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