The Role of NFATC4 Gene in Human Cutaneous Squamous Cell Carcinoma
Nuclear factor of activated T-cells, cytoplasmic 4 (NFATC4) has been implicated in keratinocyte development and several types of cancer. A well-defined role for NFATC4 in cutaneous squamous cell carcinoma (CSCC) has not yet been established. In this study, NFATC4 gene function in CSCC development wa...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Indian Journal of Dermatology |
| Subjects: | |
| Online Access: | http://www.e-ijd.org/article.asp?issn=0019-5154;year=2023;volume=68;issue=2;spage=156;epage=160;aulast=Li |
| _version_ | 1797824309285093376 |
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| author | Fengjuan Li Hongquan Chen Xiaoou Lu |
| author_facet | Fengjuan Li Hongquan Chen Xiaoou Lu |
| author_sort | Fengjuan Li |
| collection | DOAJ |
| description | Nuclear factor of activated T-cells, cytoplasmic 4 (NFATC4) has been implicated in keratinocyte development and several types of cancer. A well-defined role for NFATC4 in cutaneous squamous cell carcinoma (CSCC) has not yet been established. In this study, NFATC4 gene function in CSCC development was examined. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to measure the mRNA expression of NFATC4 in CSCC tissues and controls. A431 and Colo16 cell proliferation, invasion, and apoptosis were measured by CCK-8 assay, transwell invasion, and flow cytometry, respectively, after an NFATC4 expression lentivirus infection. Animal models were applied to validate the function of the NFATC4 gene. (1) CSCC tissues showed a significant decrease in NFATC4 expression compared to controls. (2) Overexpression of NFATc4 suppresses A431 and Colo16 cell proliferation and invasion but promotes cell apoptosis. (3) Mouse models overexpressing NFATC4 showed reduced tumourigenesis. It was suggested that NFATC4 might be a tumour suppressor gene in CSCC. |
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| format | Article |
| id | doaj.art-7187a4b965ed45808dc093b8e5f8d358 |
| institution | Directory Open Access Journal |
| issn | 0019-5154 1998-3611 |
| language | English |
| last_indexed | 2024-03-13T10:37:06Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dermatology |
| spelling | doaj.art-7187a4b965ed45808dc093b8e5f8d3582023-05-18T05:46:28ZengWolters Kluwer Medknow PublicationsIndian Journal of Dermatology0019-51541998-36112023-01-0168215616010.4103/ijd.ijd_837_22The Role of NFATC4 Gene in Human Cutaneous Squamous Cell CarcinomaFengjuan LiHongquan ChenXiaoou LuNuclear factor of activated T-cells, cytoplasmic 4 (NFATC4) has been implicated in keratinocyte development and several types of cancer. A well-defined role for NFATC4 in cutaneous squamous cell carcinoma (CSCC) has not yet been established. In this study, NFATC4 gene function in CSCC development was examined. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to measure the mRNA expression of NFATC4 in CSCC tissues and controls. A431 and Colo16 cell proliferation, invasion, and apoptosis were measured by CCK-8 assay, transwell invasion, and flow cytometry, respectively, after an NFATC4 expression lentivirus infection. Animal models were applied to validate the function of the NFATC4 gene. (1) CSCC tissues showed a significant decrease in NFATC4 expression compared to controls. (2) Overexpression of NFATc4 suppresses A431 and Colo16 cell proliferation and invasion but promotes cell apoptosis. (3) Mouse models overexpressing NFATC4 showed reduced tumourigenesis. It was suggested that NFATC4 might be a tumour suppressor gene in CSCC.http://www.e-ijd.org/article.asp?issn=0019-5154;year=2023;volume=68;issue=2;spage=156;epage=160;aulast=Licalcineurincarcinogenesiscutaneous squamous cell carcinomanfatc4 |
| spellingShingle | Fengjuan Li Hongquan Chen Xiaoou Lu The Role of NFATC4 Gene in Human Cutaneous Squamous Cell Carcinoma Indian Journal of Dermatology calcineurin carcinogenesis cutaneous squamous cell carcinoma nfatc4 |
| title | The Role of NFATC4 Gene in Human Cutaneous Squamous Cell Carcinoma |
| title_full | The Role of NFATC4 Gene in Human Cutaneous Squamous Cell Carcinoma |
| title_fullStr | The Role of NFATC4 Gene in Human Cutaneous Squamous Cell Carcinoma |
| title_full_unstemmed | The Role of NFATC4 Gene in Human Cutaneous Squamous Cell Carcinoma |
| title_short | The Role of NFATC4 Gene in Human Cutaneous Squamous Cell Carcinoma |
| title_sort | role of nfatc4 gene in human cutaneous squamous cell carcinoma |
| topic | calcineurin carcinogenesis cutaneous squamous cell carcinoma nfatc4 |
| url | http://www.e-ijd.org/article.asp?issn=0019-5154;year=2023;volume=68;issue=2;spage=156;epage=160;aulast=Li |
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