Modulation of protective reflex cough by acute immune driven inflammation of lower airways in anesthetized rabbits.
Chronic irritating cough in patients with allergic disorders may reflect behavioral or reflex response that is inappropriately matched to the stimulus present in the respiratory tract. Such dysregulated response is likely caused by sensory nerve damage driven by allergic mediators leading to cough h...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0226442 |
| _version_ | 1818588987556626432 |
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| author | Laurent Foucaud Bruno Demoulin Anne-Laure Leblanc Iulia Ioan Cyril Schweitzer Silvia Demoulin-Alexikova |
| author_facet | Laurent Foucaud Bruno Demoulin Anne-Laure Leblanc Iulia Ioan Cyril Schweitzer Silvia Demoulin-Alexikova |
| author_sort | Laurent Foucaud |
| collection | DOAJ |
| description | Chronic irritating cough in patients with allergic disorders may reflect behavioral or reflex response that is inappropriately matched to the stimulus present in the respiratory tract. Such dysregulated response is likely caused by sensory nerve damage driven by allergic mediators leading to cough hypersensitivity. Some indirect findings suggest that even acid-sensitive, capsaicin-insensitive A-δ fibers called "cough receptors" that are likely responsible for protective reflex cough may be modulated through immune driven inflammation. The aim of this study was to find out whether protective reflex cough is altered during acute allergic airway inflammation in rabbits sensitized to ovalbumin. In order to evaluate the effect of such inflammation exclusively on protective reflex cough, C-fiber mediated cough was silenced using general anesthesia. Cough provocation using citric acid inhalation and mechanical stimulation of trachea was realized in 16 ovalbumin (OVA) sensitized, anesthetized and tracheotomised rabbits 24h after OVA (OVA group, n = 9) or saline challenge (control group, n = 7). Number of coughs provoked by citric acid inhalation did not differ between OVA and control group (12,2 ±6,1 vs. 17,9 ± 6,9; p = 0.5). Allergic airway inflammation induced significant modulation of cough threshold (CT) to mechanical stimulus. Mechanically induced cough reflex in OVA group was either up-regulated (subgroup named "responders" CT: 50 msec (50-50); n = 5 p = 0.003) or down-regulated (subgroup named "non responders", CT: 1200 msec (1200-1200); n = 4 p = 0.001) when compared to control group (CT: 150 msec (75-525)). These results advocate that allergen may induce longer lasting changes of reflex cough pathway, leading to its up- or down-regulation. These findings may be of interest as they suggest that effective therapies for chronic cough in allergic patients should target sensitized component of both, reflex and behavioral cough. |
| first_indexed | 2024-12-16T09:33:29Z |
| format | Article |
| id | doaj.art-7188d218e596483eb9f1a8498816965b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:33:29Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7188d218e596483eb9f1a8498816965b2022-12-21T22:36:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011412e022644210.1371/journal.pone.0226442Modulation of protective reflex cough by acute immune driven inflammation of lower airways in anesthetized rabbits.Laurent FoucaudBruno DemoulinAnne-Laure LeblancIulia IoanCyril SchweitzerSilvia Demoulin-AlexikovaChronic irritating cough in patients with allergic disorders may reflect behavioral or reflex response that is inappropriately matched to the stimulus present in the respiratory tract. Such dysregulated response is likely caused by sensory nerve damage driven by allergic mediators leading to cough hypersensitivity. Some indirect findings suggest that even acid-sensitive, capsaicin-insensitive A-δ fibers called "cough receptors" that are likely responsible for protective reflex cough may be modulated through immune driven inflammation. The aim of this study was to find out whether protective reflex cough is altered during acute allergic airway inflammation in rabbits sensitized to ovalbumin. In order to evaluate the effect of such inflammation exclusively on protective reflex cough, C-fiber mediated cough was silenced using general anesthesia. Cough provocation using citric acid inhalation and mechanical stimulation of trachea was realized in 16 ovalbumin (OVA) sensitized, anesthetized and tracheotomised rabbits 24h after OVA (OVA group, n = 9) or saline challenge (control group, n = 7). Number of coughs provoked by citric acid inhalation did not differ between OVA and control group (12,2 ±6,1 vs. 17,9 ± 6,9; p = 0.5). Allergic airway inflammation induced significant modulation of cough threshold (CT) to mechanical stimulus. Mechanically induced cough reflex in OVA group was either up-regulated (subgroup named "responders" CT: 50 msec (50-50); n = 5 p = 0.003) or down-regulated (subgroup named "non responders", CT: 1200 msec (1200-1200); n = 4 p = 0.001) when compared to control group (CT: 150 msec (75-525)). These results advocate that allergen may induce longer lasting changes of reflex cough pathway, leading to its up- or down-regulation. These findings may be of interest as they suggest that effective therapies for chronic cough in allergic patients should target sensitized component of both, reflex and behavioral cough.https://doi.org/10.1371/journal.pone.0226442 |
| spellingShingle | Laurent Foucaud Bruno Demoulin Anne-Laure Leblanc Iulia Ioan Cyril Schweitzer Silvia Demoulin-Alexikova Modulation of protective reflex cough by acute immune driven inflammation of lower airways in anesthetized rabbits. PLoS ONE |
| title | Modulation of protective reflex cough by acute immune driven inflammation of lower airways in anesthetized rabbits. |
| title_full | Modulation of protective reflex cough by acute immune driven inflammation of lower airways in anesthetized rabbits. |
| title_fullStr | Modulation of protective reflex cough by acute immune driven inflammation of lower airways in anesthetized rabbits. |
| title_full_unstemmed | Modulation of protective reflex cough by acute immune driven inflammation of lower airways in anesthetized rabbits. |
| title_short | Modulation of protective reflex cough by acute immune driven inflammation of lower airways in anesthetized rabbits. |
| title_sort | modulation of protective reflex cough by acute immune driven inflammation of lower airways in anesthetized rabbits |
| url | https://doi.org/10.1371/journal.pone.0226442 |
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