Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in rats
Background/Objective: Epimedii Folium is the most important osteogenic herb formulated for the traditional Chinese Medicine Xian Ling Gu Bao (XLGB) capsule. The present study compared XLGB capsules containing two different Epimedium species, i.e., either Epimedium pubescens (XEP) or Epimedium korean...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-01-01
|
| Series: | Journal of Orthopaedic Translation |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214031X1500042X |
| _version_ | 1818571429139972096 |
|---|---|
| author | Shi-Hui Chen Xin-Luan Wang Li-Zhen Zheng Yi Dai Jia-Yong Zhang Bao-Lin Guo Zhi-Jun Yang Xin-Sheng Yao Ling Qin |
| author_facet | Shi-Hui Chen Xin-Luan Wang Li-Zhen Zheng Yi Dai Jia-Yong Zhang Bao-Lin Guo Zhi-Jun Yang Xin-Sheng Yao Ling Qin |
| author_sort | Shi-Hui Chen |
| collection | DOAJ |
| description | Background/Objective: Epimedii Folium is the most important osteogenic herb formulated for the traditional Chinese Medicine Xian Ling Gu Bao (XLGB) capsule. The present study compared XLGB capsules containing two different Epimedium species, i.e., either Epimedium pubescens (XEP) or Epimedium koreanum (XEK), with the focus being on the chemical constituents and antiosteoporotic efficacy.
Methods: Ultra performance liquid chromatography was used to demonstrate the different chemical constituents. Biomechanical tests, histological, and cytological evaluation were performed to characterise and compare the bone mineral density, bone strength, microstructure of bone tissue, and biological activity between XEP and XEK using an established ovariectomised (OVX) rat model.
Results: Six flavonoids with different contents between XEK and XEP were identified. As compared with the OVX group, significantly higher bone mineral density, elastic-modulus, and compressive strength were found in both the XEK group and XEP group (p < 0.05 for all, n = 8). Histomorphometric data presented significantly higher osteoblast surface ratio and osteoid area accompanied by significantly lower values of erosion surface and adiopocytes area in two treatment groups (p < 0.05, n = 6). XLGB Fufang with either XEK or XEP all showed significant preventive effects in OVX-induced osteoporosis and deterioration of bone mechanical properties.
Conclusion: The significance of the current preclinical experimental study was that these two Epimedium species used for formulating XLGB capsules were equally effective for the prevention of oestrogen-depletion induced osteoporosis. |
| first_indexed | 2024-12-14T13:55:32Z |
| format | Article |
| id | doaj.art-719634737bd24e699151a6b98a2affb3 |
| institution | Directory Open Access Journal |
| issn | 2214-031X |
| language | English |
| last_indexed | 2024-12-14T13:55:32Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Orthopaedic Translation |
| spelling | doaj.art-719634737bd24e699151a6b98a2affb32022-12-21T22:58:51ZengElsevierJournal of Orthopaedic Translation2214-031X2016-01-014C142710.1016/j.jot.2015.07.001Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in ratsShi-Hui Chen0Xin-Luan Wang1Li-Zhen Zheng2Yi Dai3Jia-Yong Zhang4Bao-Lin Guo5Zhi-Jun Yang6Xin-Sheng Yao7Ling Qin8Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR ChinaMusculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR ChinaMusculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR ChinaInstitute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, PR ChinaSchool of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, PR ChinaInstitute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR ChinaSchool of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, PR ChinaInstitute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, PR ChinaMusculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR ChinaBackground/Objective: Epimedii Folium is the most important osteogenic herb formulated for the traditional Chinese Medicine Xian Ling Gu Bao (XLGB) capsule. The present study compared XLGB capsules containing two different Epimedium species, i.e., either Epimedium pubescens (XEP) or Epimedium koreanum (XEK), with the focus being on the chemical constituents and antiosteoporotic efficacy. Methods: Ultra performance liquid chromatography was used to demonstrate the different chemical constituents. Biomechanical tests, histological, and cytological evaluation were performed to characterise and compare the bone mineral density, bone strength, microstructure of bone tissue, and biological activity between XEP and XEK using an established ovariectomised (OVX) rat model. Results: Six flavonoids with different contents between XEK and XEP were identified. As compared with the OVX group, significantly higher bone mineral density, elastic-modulus, and compressive strength were found in both the XEK group and XEP group (p < 0.05 for all, n = 8). Histomorphometric data presented significantly higher osteoblast surface ratio and osteoid area accompanied by significantly lower values of erosion surface and adiopocytes area in two treatment groups (p < 0.05, n = 6). XLGB Fufang with either XEK or XEP all showed significant preventive effects in OVX-induced osteoporosis and deterioration of bone mechanical properties. Conclusion: The significance of the current preclinical experimental study was that these two Epimedium species used for formulating XLGB capsules were equally effective for the prevention of oestrogen-depletion induced osteoporosis.http://www.sciencedirect.com/science/article/pii/S2214031X1500042XEpimediumosteoporosisovariectomised ratstreatment efficacy |
| spellingShingle | Shi-Hui Chen Xin-Luan Wang Li-Zhen Zheng Yi Dai Jia-Yong Zhang Bao-Lin Guo Zhi-Jun Yang Xin-Sheng Yao Ling Qin Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in rats Journal of Orthopaedic Translation Epimedium osteoporosis ovariectomised rats treatment efficacy |
| title | Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in rats |
| title_full | Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in rats |
| title_fullStr | Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in rats |
| title_full_unstemmed | Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in rats |
| title_short | Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in rats |
| title_sort | comparative study of two types of herbal capsules with different epimedium species for the prevention of ovariectomised induced osteoporosis in rats |
| topic | Epimedium osteoporosis ovariectomised rats treatment efficacy |
| url | http://www.sciencedirect.com/science/article/pii/S2214031X1500042X |
| work_keys_str_mv | AT shihuichen comparativestudyoftwotypesofherbalcapsuleswithdifferentepimediumspeciesforthepreventionofovariectomisedinducedosteoporosisinrats AT xinluanwang comparativestudyoftwotypesofherbalcapsuleswithdifferentepimediumspeciesforthepreventionofovariectomisedinducedosteoporosisinrats AT lizhenzheng comparativestudyoftwotypesofherbalcapsuleswithdifferentepimediumspeciesforthepreventionofovariectomisedinducedosteoporosisinrats AT yidai comparativestudyoftwotypesofherbalcapsuleswithdifferentepimediumspeciesforthepreventionofovariectomisedinducedosteoporosisinrats AT jiayongzhang comparativestudyoftwotypesofherbalcapsuleswithdifferentepimediumspeciesforthepreventionofovariectomisedinducedosteoporosisinrats AT baolinguo comparativestudyoftwotypesofherbalcapsuleswithdifferentepimediumspeciesforthepreventionofovariectomisedinducedosteoporosisinrats AT zhijunyang comparativestudyoftwotypesofherbalcapsuleswithdifferentepimediumspeciesforthepreventionofovariectomisedinducedosteoporosisinrats AT xinshengyao comparativestudyoftwotypesofherbalcapsuleswithdifferentepimediumspeciesforthepreventionofovariectomisedinducedosteoporosisinrats AT lingqin comparativestudyoftwotypesofherbalcapsuleswithdifferentepimediumspeciesforthepreventionofovariectomisedinducedosteoporosisinrats |