| Summary: | <i>Moringa oleifera</i> Lam. is rich in phytochemical compounds especially glucosinolates (GSs) and isothiocyanates (ITCs), which are active compounds for cancer chemoprevention benefits of Brassicaceae vegetables. In this study, we determined the total contents of GSs and ITCs and their specific profiles in different <i>Moringa</i> tissues including seeds, stems, leaves and roots. Seeds (seeds with shell and seed kernel) showed significantly higher levels of total GSs and ITCs than that of other <i>Moringa</i> tissues. The hydrogen sulfide (H<sub>2</sub>S) releasing capacity of total ITCs extracted from different <i>Moringa</i> tissues was determined by lead (II) acetate assay in 24-well plates. The H<sub>2</sub>S releasing capacity of different <i>Moringa</i> tissues were evaluated and compared. <i>Moringa</i> seeds showed the highest H<sub>2</sub>S releasing capacity, followed by roots, leaves and stems. Our results suggest that <i>Moringa</i> based foods may exhibit health benefits due to its GSs and ITCs contents that are the precursors for H<sub>2</sub>S, in addition to the recognized action mechanisms of ITCs.
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