A map of cell type‐specific auxin responses
In plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same auxin stimulus. However, little is known about how auxin is interpreted by individual cell types. We performed a transcriptomic analysis of responses to...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2013-01-01
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| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb.2013.40 |
| _version_ | 1797280447794774016 |
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| author | Bastiaan O R Bargmann Steffen Vanneste Gabriel Krouk Tal Nawy Idan Efroni Eilon Shani Goh Choe Jiří Friml Dominique C Bergmann Mark Estelle Kenneth D Birnbaum |
| author_facet | Bastiaan O R Bargmann Steffen Vanneste Gabriel Krouk Tal Nawy Idan Efroni Eilon Shani Goh Choe Jiří Friml Dominique C Bergmann Mark Estelle Kenneth D Birnbaum |
| author_sort | Bastiaan O R Bargmann |
| collection | DOAJ |
| description | In plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same auxin stimulus. However, little is known about how auxin is interpreted by individual cell types. We performed a transcriptomic analysis of responses to auxin within four distinct tissues of the Arabidopsis thaliana root and demonstrate that different cell types show competence for discrete responses. The majority of auxin‐responsive genes displayed a spatial bias in their induction or repression. The novel data set was used to examine how auxin influences tissue‐specific transcriptional regulation of cell‐identity markers. Additionally, the data were used in combination with spatial expression maps of the root to plot a transcriptomic auxin‐response gradient across the apical and basal meristem. The readout revealed a strong correlation for thousands of genes between the relative response to auxin and expression along the longitudinal axis of the root. This data set and comparative analysis provide a transcriptome‐level spatial breakdown of the response to auxin within an organ where this hormone mediates many aspects of development. |
| first_indexed | 2024-03-07T16:41:21Z |
| format | Article |
| id | doaj.art-7199349a5d9b4841bcd77d6b80d2f533 |
| institution | Directory Open Access Journal |
| issn | 1744-4292 |
| language | English |
| last_indexed | 2024-03-07T16:41:21Z |
| publishDate | 2013-01-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj.art-7199349a5d9b4841bcd77d6b80d2f5332024-03-03T08:20:00ZengSpringer NatureMolecular Systems Biology1744-42922013-01-0191n/an/a10.1038/msb.2013.40A map of cell type‐specific auxin responsesBastiaan O R Bargmann0Steffen Vanneste1Gabriel Krouk2Tal Nawy3Idan Efroni4Eilon Shani5Goh Choe6Jiří Friml7Dominique C Bergmann8Mark Estelle9Kenneth D Birnbaum10Biology Department, Center for Genomics and Systems Biology, New York University New York NY USADepartment of Plant Systems Biology, VIB Ghent BelgiumLaboratoire de Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes—Claude Grignon Montpellier FranceBiology Department, Center for Genomics and Systems Biology, New York University New York NY USABiology Department, Center for Genomics and Systems Biology, New York University New York NY USADepartment of Cell and Developmental Biology, UCSD La Jolla CA USADepartment of Cell and Developmental Biology, UCSD La Jolla CA USADepartment of Plant Systems Biology, VIB Ghent BelgiumHoward Hughes Medical Institute, Stanford University, Stanford CA USADepartment of Cell and Developmental Biology, UCSD La Jolla CA USABiology Department, Center for Genomics and Systems Biology, New York University New York NY USAIn plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same auxin stimulus. However, little is known about how auxin is interpreted by individual cell types. We performed a transcriptomic analysis of responses to auxin within four distinct tissues of the Arabidopsis thaliana root and demonstrate that different cell types show competence for discrete responses. The majority of auxin‐responsive genes displayed a spatial bias in their induction or repression. The novel data set was used to examine how auxin influences tissue‐specific transcriptional regulation of cell‐identity markers. Additionally, the data were used in combination with spatial expression maps of the root to plot a transcriptomic auxin‐response gradient across the apical and basal meristem. The readout revealed a strong correlation for thousands of genes between the relative response to auxin and expression along the longitudinal axis of the root. This data set and comparative analysis provide a transcriptome‐level spatial breakdown of the response to auxin within an organ where this hormone mediates many aspects of development.https://doi.org/10.1038/msb.2013.40Arabidopsisdevelopmentroot apical meristemsignaling gradient |
| spellingShingle | Bastiaan O R Bargmann Steffen Vanneste Gabriel Krouk Tal Nawy Idan Efroni Eilon Shani Goh Choe Jiří Friml Dominique C Bergmann Mark Estelle Kenneth D Birnbaum A map of cell type‐specific auxin responses Molecular Systems Biology Arabidopsis development root apical meristem signaling gradient |
| title | A map of cell type‐specific auxin responses |
| title_full | A map of cell type‐specific auxin responses |
| title_fullStr | A map of cell type‐specific auxin responses |
| title_full_unstemmed | A map of cell type‐specific auxin responses |
| title_short | A map of cell type‐specific auxin responses |
| title_sort | map of cell type specific auxin responses |
| topic | Arabidopsis development root apical meristem signaling gradient |
| url | https://doi.org/10.1038/msb.2013.40 |
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