In Vitro Assembly of Virus-Like Particles and Their Applications
Virus-like particles (VLPs) are increasingly used for vaccine development and drug delivery. Assembly of VLPs from purified monomers in a chemically defined reaction is advantageous compared to in vivo assembly, because it avoids encapsidation of host-derived components and enables loading with adde...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/4/334 |
| _version_ | 1797538129218895872 |
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| author | Dinh To Le Kristian M. Müller |
| author_facet | Dinh To Le Kristian M. Müller |
| author_sort | Dinh To Le |
| collection | DOAJ |
| description | Virus-like particles (VLPs) are increasingly used for vaccine development and drug delivery. Assembly of VLPs from purified monomers in a chemically defined reaction is advantageous compared to in vivo assembly, because it avoids encapsidation of host-derived components and enables loading with added cargoes. This review provides an overview of ex cella VLP production methods focusing on capsid protein production, factors that impact the in vitro assembly, and approaches to characterize in vitro VLPs. The uses of in vitro produced VLPs as vaccines and for therapeutic delivery are also reported. |
| first_indexed | 2024-03-10T12:26:06Z |
| format | Article |
| id | doaj.art-719a5dd525394023b80c8ebfa377421b |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T12:26:06Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-719a5dd525394023b80c8ebfa377421b2023-11-21T15:00:18ZengMDPI AGLife2075-17292021-04-0111433410.3390/life11040334In Vitro Assembly of Virus-Like Particles and Their ApplicationsDinh To Le0Kristian M. Müller1Cellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, 33615 Bielefeld, GermanyCellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, 33615 Bielefeld, GermanyVirus-like particles (VLPs) are increasingly used for vaccine development and drug delivery. Assembly of VLPs from purified monomers in a chemically defined reaction is advantageous compared to in vivo assembly, because it avoids encapsidation of host-derived components and enables loading with added cargoes. This review provides an overview of ex cella VLP production methods focusing on capsid protein production, factors that impact the in vitro assembly, and approaches to characterize in vitro VLPs. The uses of in vitro produced VLPs as vaccines and for therapeutic delivery are also reported.https://www.mdpi.com/2075-1729/11/4/334capsid proteinin vitro assemblyvirus-like particleVLP-based vaccinesdrug delivery |
| spellingShingle | Dinh To Le Kristian M. Müller In Vitro Assembly of Virus-Like Particles and Their Applications Life capsid protein in vitro assembly virus-like particle VLP-based vaccines drug delivery |
| title | In Vitro Assembly of Virus-Like Particles and Their Applications |
| title_full | In Vitro Assembly of Virus-Like Particles and Their Applications |
| title_fullStr | In Vitro Assembly of Virus-Like Particles and Their Applications |
| title_full_unstemmed | In Vitro Assembly of Virus-Like Particles and Their Applications |
| title_short | In Vitro Assembly of Virus-Like Particles and Their Applications |
| title_sort | in vitro assembly of virus like particles and their applications |
| topic | capsid protein in vitro assembly virus-like particle VLP-based vaccines drug delivery |
| url | https://www.mdpi.com/2075-1729/11/4/334 |
| work_keys_str_mv | AT dinhtole invitroassemblyofviruslikeparticlesandtheirapplications AT kristianmmuller invitroassemblyofviruslikeparticlesandtheirapplications |