Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding
Semi-dwarfing improves the lodging resistance and yield of rice, and the vast majority of modern rice varieties harbor the sd1 allele to decrease plant height, resulting in reduced genetic diversity and negative agronomic traits. Thus, exploring alternative sources of dwarfism is imperative for rice...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-11-01
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| Series: | Rice Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630822000750 |
| _version_ | 1798019491037184000 |
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| author | Liu Yantong Li Ting Jiang Zhishu Zeng Chuihai He Rong Qiu Jiao Lin Xiaoli Peng Limei Song Yongping Zhou Dahu Cai Yicong Zhu Changlan Fu Junru He Haohua Xu Jie |
| author_facet | Liu Yantong Li Ting Jiang Zhishu Zeng Chuihai He Rong Qiu Jiao Lin Xiaoli Peng Limei Song Yongping Zhou Dahu Cai Yicong Zhu Changlan Fu Junru He Haohua Xu Jie |
| author_sort | Liu Yantong |
| collection | DOAJ |
| description | Semi-dwarfing improves the lodging resistance and yield of rice, and the vast majority of modern rice varieties harbor the sd1 allele to decrease plant height, resulting in reduced genetic diversity and negative agronomic traits. Thus, exploring alternative sources of dwarfism is imperative for rice breeding. Here, we identified a novel RGA1 allele, d1-w, from a local indica variety Xiaolixiang (XLX) using a map-based cloning approach. Compared with other rice varieties, RGA1 in XLX contained a unique single nucleotide polymorphism that resulted in an additional transcript and reduced functional RGA1 transcript level. The RGA1 from Nipponbare was introduced into XLX to estimate the value of d1-w in rice breeding. Compared with transgenic XLX plants (XLXD1), XLX exhibited reduced plant height, increased stem strength, lower reactive oxygen species accumulation, delayed senescence, stronger photosynthesis, higher grain yield and quality (including external, milling and nutritional qualities), and enhanced resistance to drought and Rhizoctonia solani. Therefore, we proposed that the d1-w allele has potential as an excellent dwarfism resource for rice breeding. |
| first_indexed | 2024-04-11T16:41:37Z |
| format | Article |
| id | doaj.art-719bcc9de1aa43039ae8682a26f34102 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-04-11T16:41:37Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj.art-719bcc9de1aa43039ae8682a26f341022022-12-22T04:13:40ZengElsevierRice Science1672-63082022-11-01296522534Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice BreedingLiu Yantong0Li Ting1Jiang Zhishu2Zeng Chuihai3He Rong4Qiu Jiao5Lin Xiaoli6Peng Limei7Song Yongping8Zhou Dahu9Cai Yicong10Zhu Changlan11Fu Junru12He Haohua13Xu Jie14Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaCorresponding author: Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China,; Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaCorresponding author: Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China,; Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaSemi-dwarfing improves the lodging resistance and yield of rice, and the vast majority of modern rice varieties harbor the sd1 allele to decrease plant height, resulting in reduced genetic diversity and negative agronomic traits. Thus, exploring alternative sources of dwarfism is imperative for rice breeding. Here, we identified a novel RGA1 allele, d1-w, from a local indica variety Xiaolixiang (XLX) using a map-based cloning approach. Compared with other rice varieties, RGA1 in XLX contained a unique single nucleotide polymorphism that resulted in an additional transcript and reduced functional RGA1 transcript level. The RGA1 from Nipponbare was introduced into XLX to estimate the value of d1-w in rice breeding. Compared with transgenic XLX plants (XLXD1), XLX exhibited reduced plant height, increased stem strength, lower reactive oxygen species accumulation, delayed senescence, stronger photosynthesis, higher grain yield and quality (including external, milling and nutritional qualities), and enhanced resistance to drought and Rhizoctonia solani. Therefore, we proposed that the d1-w allele has potential as an excellent dwarfism resource for rice breeding.http://www.sciencedirect.com/science/article/pii/S1672630822000750riceweak alleleRGA1dwarf germplasm resourceyieldgrain quality |
| spellingShingle | Liu Yantong Li Ting Jiang Zhishu Zeng Chuihai He Rong Qiu Jiao Lin Xiaoli Peng Limei Song Yongping Zhou Dahu Cai Yicong Zhu Changlan Fu Junru He Haohua Xu Jie Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding Rice Science rice weak allele RGA1 dwarf germplasm resource yield grain quality |
| title | Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding |
| title_full | Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding |
| title_fullStr | Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding |
| title_full_unstemmed | Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding |
| title_short | Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding |
| title_sort | characterization of a novel weak allele of rga1 d1 and its potential application in rice breeding |
| topic | rice weak allele RGA1 dwarf germplasm resource yield grain quality |
| url | http://www.sciencedirect.com/science/article/pii/S1672630822000750 |
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