Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma
Introduction: Cancer has multifactorial aetiology and is a multistep process involving initiation, promotion and tumour progression. Cellular proliferation is one of the important indicators for the biologic aggressiveness of a malignant lesion. The dysregulated proliferation may be a significan...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-06-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/6094/12645_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
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| author | Chandrashekar Poosarla Maya Ramesh K. Ramesh Swetha Gudiseva Sekar Bala Murali Sundar |
| author_facet | Chandrashekar Poosarla Maya Ramesh K. Ramesh Swetha Gudiseva Sekar Bala Murali Sundar |
| author_sort | Chandrashekar Poosarla |
| collection | DOAJ |
| description | Introduction: Cancer has multifactorial aetiology and is a multistep
process involving initiation, promotion and tumour progression.
Cellular proliferation is one of the important indicators for the
biologic aggressiveness of a malignant lesion. The dysregulated
proliferation may be a significant change to determine the potential
prognosis of various malignant tumours.
Aim: The aim of this study was to evaluate the expression of
proliferating cell nuclear antigen (PCNA) as an indicator for
clinical aggressiveness in oral premalignancy and squamous cell
carcinoma.
Materials and Methods: A total of 50 blocks were taken from the
Department of Oral Pathology which was diagnosed previously
histopathologically. It comprised of normal oral mucosa (10),
dysplasia (10) and grades of oral squamous cell carcinoma (30)
of patients between the age group of 40–60 years. From each
block, sections of 4 micro metre thicknesses were prepared
and placed on poly- L lysine coated slides. These sections were
immunohistochemically stained with monoclonal proliferating cell
antibody (PC10). The stained slides were evaluated by a single
examiner for cell count.
Results: A comparison between study groups and controls
showed a probability value (p-value) < 0.05. Significant increase
in the proliferative index from the normal to oral squamous cell
carcinoma was noticed. Poorly differentiated squamous cell
carcinoma showed maximum proliferative index followed by
moderately differentiated, well differentiated squamous cell
carcinoma, dysplasia and normal mucosa.
Conclusion: Present study concluded that PCNA index can be
used to assess the proliferation and aggressiveness in dysplasia
and different grades oral squamous cell carcinoma. |
| first_indexed | 2024-12-16T07:02:35Z |
| format | Article |
| id | doaj.art-719c5441e8db4728b2bcf0e02d54acda |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-16T07:02:35Z |
| publishDate | 2015-06-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-719c5441e8db4728b2bcf0e02d54acda2022-12-21T22:40:07ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-06-0196ZC39ZC4110.7860/JCDR/2015/12645.6094Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell CarcinomaChandrashekar Poosarla0Maya Ramesh1K. Ramesh2Swetha Gudiseva3Sekar Bala4Murali Sundar5Reader, Department of Oral Pathology, SIBAR Institute of Dental Sciences, Guntur, Andhra Pradesh, India.Reader, Department of Oral Pathology, Vinayaka Missions Sankarachariyar Dental College, Salem, Tamil Nadu, India. Professor, Department of Pedodontics, Vinayaka Missions Sankarachariyar Dental college, Salem, Tamil Nadu, India. Dental Practioner, Guntur, Andhra Pradesh, India. Professor, Department of Oral Pathology, Vinayaka Missions Sankarachariyar Dental college, Salem, Tamil Nadu, India.Professor, Department of Oral Pathology, Vinayaka Missions Sankarachariyar Dental college, Salem, Tamil Nadu, India.Introduction: Cancer has multifactorial aetiology and is a multistep process involving initiation, promotion and tumour progression. Cellular proliferation is one of the important indicators for the biologic aggressiveness of a malignant lesion. The dysregulated proliferation may be a significant change to determine the potential prognosis of various malignant tumours. Aim: The aim of this study was to evaluate the expression of proliferating cell nuclear antigen (PCNA) as an indicator for clinical aggressiveness in oral premalignancy and squamous cell carcinoma. Materials and Methods: A total of 50 blocks were taken from the Department of Oral Pathology which was diagnosed previously histopathologically. It comprised of normal oral mucosa (10), dysplasia (10) and grades of oral squamous cell carcinoma (30) of patients between the age group of 40–60 years. From each block, sections of 4 micro metre thicknesses were prepared and placed on poly- L lysine coated slides. These sections were immunohistochemically stained with monoclonal proliferating cell antibody (PC10). The stained slides were evaluated by a single examiner for cell count. Results: A comparison between study groups and controls showed a probability value (p-value) < 0.05. Significant increase in the proliferative index from the normal to oral squamous cell carcinoma was noticed. Poorly differentiated squamous cell carcinoma showed maximum proliferative index followed by moderately differentiated, well differentiated squamous cell carcinoma, dysplasia and normal mucosa. Conclusion: Present study concluded that PCNA index can be used to assess the proliferation and aggressiveness in dysplasia and different grades oral squamous cell carcinoma.https://jcdr.net/articles/PDF/6094/12645_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdfdysplasiaimmunohistochemistryoral cancer |
| spellingShingle | Chandrashekar Poosarla Maya Ramesh K. Ramesh Swetha Gudiseva Sekar Bala Murali Sundar Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma Journal of Clinical and Diagnostic Research dysplasia immunohistochemistry oral cancer |
| title | Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma |
| title_full | Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma |
| title_fullStr | Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma |
| title_short | Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma |
| title_sort | proliferating cell nuclear antigen in premalignancy and oral squamous cell carcinoma |
| topic | dysplasia immunohistochemistry oral cancer |
| url | https://jcdr.net/articles/PDF/6094/12645_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
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