Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview
With the advent of molecular technology, several isothermal techniques for rapid detection of zoonotic pathogens have been developed. Among them, recombinase polymerase amplification (RPA) is becoming an important technology for rapid, sensitive, and economical detection of zoonotic pathogens. RPA t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Compuscript Ltd
2022-02-01
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| Series: | Zoonoses |
| Online Access: | https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2022-0002 |
| _version_ | 1797399428891410432 |
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| author | Ruichen Lv Nianhong Lu Junhu Wang Yuexi Li Yong Qi |
| author_facet | Ruichen Lv Nianhong Lu Junhu Wang Yuexi Li Yong Qi |
| author_sort | Ruichen Lv |
| collection | DOAJ |
| description | With the advent of molecular technology, several isothermal techniques for rapid detection of zoonotic pathogens have been developed. Among them, recombinase polymerase amplification (RPA) is becoming an important technology for rapid, sensitive, and economical detection of zoonotic pathogens. RPA technology has the advantage of being able to be implemented in field settings, because the method requires minimal sample preparation and is performed at a constant low temperature (37–42°C). RPA is rapidly becoming a promising tool for the rapid detection, prevention, and control of zoonotic diseases. This article discusses the principles of RPA technology and its derivatives, including RPA coupled with lateral flow testing (RPA-LF), real-time fluorescence RPA, electrochemical RPA, and flocculation RPA, and their applications in the detection of zoonotic pathogens. |
| first_indexed | 2024-03-09T01:40:08Z |
| format | Article |
| id | doaj.art-719c8f84cdfc421f9a331b5edf27a761 |
| institution | Directory Open Access Journal |
| issn | 2737-7466 2737-7474 |
| language | English |
| last_indexed | 2024-03-09T01:40:08Z |
| publishDate | 2022-02-01 |
| publisher | Compuscript Ltd |
| record_format | Article |
| series | Zoonoses |
| spelling | doaj.art-719c8f84cdfc421f9a331b5edf27a7612023-12-08T17:00:55ZengCompuscript LtdZoonoses2737-74662737-74742022-02-012199010.15212/ZOONOSES-2022-0002Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An OverviewRuichen LvNianhong LuJunhu WangYuexi LiYong QiWith the advent of molecular technology, several isothermal techniques for rapid detection of zoonotic pathogens have been developed. Among them, recombinase polymerase amplification (RPA) is becoming an important technology for rapid, sensitive, and economical detection of zoonotic pathogens. RPA technology has the advantage of being able to be implemented in field settings, because the method requires minimal sample preparation and is performed at a constant low temperature (37–42°C). RPA is rapidly becoming a promising tool for the rapid detection, prevention, and control of zoonotic diseases. This article discusses the principles of RPA technology and its derivatives, including RPA coupled with lateral flow testing (RPA-LF), real-time fluorescence RPA, electrochemical RPA, and flocculation RPA, and their applications in the detection of zoonotic pathogens.https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2022-0002 |
| spellingShingle | Ruichen Lv Nianhong Lu Junhu Wang Yuexi Li Yong Qi Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview Zoonoses |
| title | Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview |
| title_full | Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview |
| title_fullStr | Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview |
| title_full_unstemmed | Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview |
| title_short | Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview |
| title_sort | recombinase polymerase amplification for rapid detection of zoonotic pathogens an overview |
| url | https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2022-0002 |
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