Divergent roles for Ly6C+CCR2+CX3CR1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen Leishmania major.
Inflammatory monocytes can be manipulated by environmental cues to perform multiple functions. To define the role of monocytes during primary or secondary infection with an intra-phagosomal pathogen we employed Leishmania major-red fluorescent protein (RFP) parasites and multi-color flow cytometry t...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-06-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1006479 |
| _version_ | 1819145858876178432 |
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| author | Audrey Romano Matheus B H Carneiro Nicole A Doria Eric H Roma Flavia L Ribeiro-Gomes Ehud Inbar Sang Hun Lee Jonatan Mendez Andrea Paun David L Sacks Nathan C Peters |
| author_facet | Audrey Romano Matheus B H Carneiro Nicole A Doria Eric H Roma Flavia L Ribeiro-Gomes Ehud Inbar Sang Hun Lee Jonatan Mendez Andrea Paun David L Sacks Nathan C Peters |
| author_sort | Audrey Romano |
| collection | DOAJ |
| description | Inflammatory monocytes can be manipulated by environmental cues to perform multiple functions. To define the role of monocytes during primary or secondary infection with an intra-phagosomal pathogen we employed Leishmania major-red fluorescent protein (RFP) parasites and multi-color flow cytometry to define and enumerate infected and uninfected inflammatory cells in the skin. During primary infection, infected monocytes had altered maturation and were the initial mononuclear host cell for parasite replication. In contrast, at a distal site of secondary infection in mice with a healed but persistent primary infection, this same population rapidly produced inducible nitric oxide synthase (iNOS) in an IFN-γ dependent manner and was critical for parasite killing. Maturation to a dendritic cell-like phenotype was not required for monocyte iNOS-production, and enhanced monocyte recruitment correlated with IFN-γ dependent cxcl10 expression. In contrast, neutrophils appeared to be a safe haven for parasites in both primary and secondary sites. Thus, inflammatory monocytes play divergent roles during primary versus secondary infection with an intra-phagosomal pathogen. |
| first_indexed | 2024-12-22T13:04:43Z |
| format | Article |
| id | doaj.art-719e457dc0f44cb48b46e5d060e60811 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-22T13:04:43Z |
| publishDate | 2017-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-719e457dc0f44cb48b46e5d060e608112022-12-21T18:24:55ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742017-06-01136e100647910.1371/journal.ppat.1006479Divergent roles for Ly6C+CCR2+CX3CR1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen Leishmania major.Audrey RomanoMatheus B H CarneiroNicole A DoriaEric H RomaFlavia L Ribeiro-GomesEhud InbarSang Hun LeeJonatan MendezAndrea PaunDavid L SacksNathan C PetersInflammatory monocytes can be manipulated by environmental cues to perform multiple functions. To define the role of monocytes during primary or secondary infection with an intra-phagosomal pathogen we employed Leishmania major-red fluorescent protein (RFP) parasites and multi-color flow cytometry to define and enumerate infected and uninfected inflammatory cells in the skin. During primary infection, infected monocytes had altered maturation and were the initial mononuclear host cell for parasite replication. In contrast, at a distal site of secondary infection in mice with a healed but persistent primary infection, this same population rapidly produced inducible nitric oxide synthase (iNOS) in an IFN-γ dependent manner and was critical for parasite killing. Maturation to a dendritic cell-like phenotype was not required for monocyte iNOS-production, and enhanced monocyte recruitment correlated with IFN-γ dependent cxcl10 expression. In contrast, neutrophils appeared to be a safe haven for parasites in both primary and secondary sites. Thus, inflammatory monocytes play divergent roles during primary versus secondary infection with an intra-phagosomal pathogen.https://doi.org/10.1371/journal.ppat.1006479 |
| spellingShingle | Audrey Romano Matheus B H Carneiro Nicole A Doria Eric H Roma Flavia L Ribeiro-Gomes Ehud Inbar Sang Hun Lee Jonatan Mendez Andrea Paun David L Sacks Nathan C Peters Divergent roles for Ly6C+CCR2+CX3CR1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen Leishmania major. PLoS Pathogens |
| title | Divergent roles for Ly6C+CCR2+CX3CR1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen Leishmania major. |
| title_full | Divergent roles for Ly6C+CCR2+CX3CR1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen Leishmania major. |
| title_fullStr | Divergent roles for Ly6C+CCR2+CX3CR1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen Leishmania major. |
| title_full_unstemmed | Divergent roles for Ly6C+CCR2+CX3CR1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen Leishmania major. |
| title_short | Divergent roles for Ly6C+CCR2+CX3CR1+ inflammatory monocytes during primary or secondary infection of the skin with the intra-phagosomal pathogen Leishmania major. |
| title_sort | divergent roles for ly6c ccr2 cx3cr1 inflammatory monocytes during primary or secondary infection of the skin with the intra phagosomal pathogen leishmania major |
| url | https://doi.org/10.1371/journal.ppat.1006479 |
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