Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasm
Agaves have been a valuable resource in dryland areas for centuries, providing fibers (sisal), food, and beverages. However, the advent of synthetic fibers has led to a decrease in research on Agave, resulting in the cessation of breeding programs in Brazil. With the rise of climate change, there is...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Chemical Engineering |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fceng.2023.1218668/full |
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| author | Fabio Trigo Raya Fabio Trigo Raya Lucas Miguel de Carvalho Juliana José Larissa Prado da Cruz Rafael Leonardo Almeida Heliur Alves de Almeida Delevatti Neidiquele Maria Silveira Simone Ferreira da Silva Maria Dolores Pissolato Adriele Bárbara de Oliveira Wagner José Villela dos Reis Luís Guilherme Furlan de Abreu Jesús Gutiérrez Marcelo Falsarella Carazzolle Ana Cristina Fermino Soares Jorge Nieto Sotelo Rafael Vasconcelos Ribeiro Gonçalo Amarante Guimarães Pereira |
| author_facet | Fabio Trigo Raya Fabio Trigo Raya Lucas Miguel de Carvalho Juliana José Larissa Prado da Cruz Rafael Leonardo Almeida Heliur Alves de Almeida Delevatti Neidiquele Maria Silveira Simone Ferreira da Silva Maria Dolores Pissolato Adriele Bárbara de Oliveira Wagner José Villela dos Reis Luís Guilherme Furlan de Abreu Jesús Gutiérrez Marcelo Falsarella Carazzolle Ana Cristina Fermino Soares Jorge Nieto Sotelo Rafael Vasconcelos Ribeiro Gonçalo Amarante Guimarães Pereira |
| author_sort | Fabio Trigo Raya |
| collection | DOAJ |
| description | Agaves have been a valuable resource in dryland areas for centuries, providing fibers (sisal), food, and beverages. However, the advent of synthetic fibers has led to a decrease in research on Agave, resulting in the cessation of breeding programs in Brazil. With the rise of climate change, there is renewed interest in Agave for its potential as a biofuel feedstock in semiarid regions. Since 2016, we have been collecting Agave accessions throughout the country and retrieving what is left of Brazil’s original breeding program to establish a new germplasm bank. Here, we evaluated 21 of those accessions growing in the field. We used molecular markers and morphophysiological traits to characterize the plants. Based on the Mayahuelin molecular marker, we were able to reconstruct a phylogeny for the Brazilian accessions. The morphophysiological traits explained 34.6% of the phenotypic variation in the dataset, with physiological traits such as leaf water content, effective quantum efficiency of photosystem II (ΦPSII), and specific leaf mass (SLM) as the most significant traits. Specifically, we evaluated nine Agave species and found that the physiological traits, rather than the morphological ones, were the most significant. Leaf water content was negatively correlated with specific leaf mass, which could be used as a marker for selecting cultivars with higher biomass accumulation. Interestingly, ΦPSII and chlorophyll content were negatively correlated, suggesting photochemical adaptations throughout the rosette. Molecular and phenotypic data suggest that A. amaniensis, which is frequently considered a synonym of A. sisalana, is effectively another species. Overall, this study provides valuable information on the physiological traits of Brazilian Agave accessions and is a starting point for selecting more productive and climate-resilient cultivars for biorenewables production. |
| first_indexed | 2024-03-11T21:29:55Z |
| format | Article |
| id | doaj.art-71a00dc541134b6998210de4338096fa |
| institution | Directory Open Access Journal |
| issn | 2673-2718 |
| language | English |
| last_indexed | 2024-03-11T21:29:55Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemical Engineering |
| spelling | doaj.art-71a00dc541134b6998210de4338096fa2023-09-27T11:02:29ZengFrontiers Media S.A.Frontiers in Chemical Engineering2673-27182023-09-01510.3389/fceng.2023.12186681218668Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasmFabio Trigo Raya0Fabio Trigo Raya1Lucas Miguel de Carvalho2Juliana José3Larissa Prado da Cruz4Rafael Leonardo Almeida5Heliur Alves de Almeida Delevatti6Neidiquele Maria Silveira7Simone Ferreira da Silva8Maria Dolores Pissolato9Adriele Bárbara de Oliveira10Wagner José Villela dos Reis11Luís Guilherme Furlan de Abreu12Jesús Gutiérrez13Marcelo Falsarella Carazzolle14Ana Cristina Fermino Soares15Jorge Nieto Sotelo16Rafael Vasconcelos Ribeiro17Gonçalo Amarante Guimarães Pereira18Department of Genetics, Evolution, Microbiology and Immunology, Laboratory of Genomics and BioEnergy (LGE), University of Campinas (Unicamp), Campinas, BrazilDepartment of Plant Biology, Laboratory of Crop Physiology (LCroP), University of Campinas (Unicamp), Campinas, BrazilDepartment of Plant Biology, Laboratory of Crop Physiology (LCroP), University of Campinas (Unicamp), Campinas, BrazilDepartment of Genetics, Evolution, Microbiology and Immunology, Laboratory of Genomics and BioEnergy (LGE), University of Campinas (Unicamp), Campinas, BrazilDepartment of Plant Biology, Laboratory of Crop Physiology (LCroP), University of Campinas (Unicamp), Campinas, BrazilDepartment of Plant Biology, Laboratory of Crop Physiology (LCroP), University of Campinas (Unicamp), Campinas, BrazilDepartment of Plant Biology, Laboratory of Crop Physiology (LCroP), University of Campinas (Unicamp), Campinas, BrazilDepartment of Plant Biology, Laboratory of Crop Physiology (LCroP), University of Campinas (Unicamp), Campinas, BrazilDepartment of Plant Biology, Laboratory of Crop Physiology (LCroP), University of Campinas (Unicamp), Campinas, BrazilDepartment of Plant Biology, Laboratory of Crop Physiology (LCroP), University of Campinas (Unicamp), Campinas, BrazilDepartment of Genetics, Evolution, Microbiology and Immunology, Laboratory of Genomics and BioEnergy (LGE), University of Campinas (Unicamp), Campinas, BrazilDepartment of Genetics, Evolution, Microbiology and Immunology, Laboratory of Genomics and BioEnergy (LGE), University of Campinas (Unicamp), Campinas, BrazilDepartment of Genetics, Evolution, Microbiology and Immunology, Laboratory of Genomics and BioEnergy (LGE), University of Campinas (Unicamp), Campinas, BrazilBotanical Garden, Institute of Biology, National Autonomous University of Mexico, Mexico City, MexicoDepartment of Genetics, Evolution, Microbiology and Immunology, Laboratory of Genomics and BioEnergy (LGE), University of Campinas (Unicamp), Campinas, BrazilCenter of Agricultural, Environmental and Biological Sciences, Federal University of Recôncavo of Bahia (UFRB), Cruz das Almas, BrazilBotanical Garden, Institute of Biology, National Autonomous University of Mexico, Mexico City, MexicoDepartment of Plant Biology, Laboratory of Crop Physiology (LCroP), University of Campinas (Unicamp), Campinas, BrazilDepartment of Genetics, Evolution, Microbiology and Immunology, Laboratory of Genomics and BioEnergy (LGE), University of Campinas (Unicamp), Campinas, BrazilAgaves have been a valuable resource in dryland areas for centuries, providing fibers (sisal), food, and beverages. However, the advent of synthetic fibers has led to a decrease in research on Agave, resulting in the cessation of breeding programs in Brazil. With the rise of climate change, there is renewed interest in Agave for its potential as a biofuel feedstock in semiarid regions. Since 2016, we have been collecting Agave accessions throughout the country and retrieving what is left of Brazil’s original breeding program to establish a new germplasm bank. Here, we evaluated 21 of those accessions growing in the field. We used molecular markers and morphophysiological traits to characterize the plants. Based on the Mayahuelin molecular marker, we were able to reconstruct a phylogeny for the Brazilian accessions. The morphophysiological traits explained 34.6% of the phenotypic variation in the dataset, with physiological traits such as leaf water content, effective quantum efficiency of photosystem II (ΦPSII), and specific leaf mass (SLM) as the most significant traits. Specifically, we evaluated nine Agave species and found that the physiological traits, rather than the morphological ones, were the most significant. Leaf water content was negatively correlated with specific leaf mass, which could be used as a marker for selecting cultivars with higher biomass accumulation. Interestingly, ΦPSII and chlorophyll content were negatively correlated, suggesting photochemical adaptations throughout the rosette. Molecular and phenotypic data suggest that A. amaniensis, which is frequently considered a synonym of A. sisalana, is effectively another species. Overall, this study provides valuable information on the physiological traits of Brazilian Agave accessions and is a starting point for selecting more productive and climate-resilient cultivars for biorenewables production.https://www.frontiersin.org/articles/10.3389/fceng.2023.1218668/fullAgaveBiofuelsgenetic resourcesnatural fiberssisal |
| spellingShingle | Fabio Trigo Raya Fabio Trigo Raya Lucas Miguel de Carvalho Juliana José Larissa Prado da Cruz Rafael Leonardo Almeida Heliur Alves de Almeida Delevatti Neidiquele Maria Silveira Simone Ferreira da Silva Maria Dolores Pissolato Adriele Bárbara de Oliveira Wagner José Villela dos Reis Luís Guilherme Furlan de Abreu Jesús Gutiérrez Marcelo Falsarella Carazzolle Ana Cristina Fermino Soares Jorge Nieto Sotelo Rafael Vasconcelos Ribeiro Gonçalo Amarante Guimarães Pereira Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasm Frontiers in Chemical Engineering Agave Biofuels genetic resources natural fibers sisal |
| title | Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasm |
| title_full | Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasm |
| title_fullStr | Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasm |
| title_full_unstemmed | Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasm |
| title_short | Rescuing the Brazilian Agave breeding program: morphophysiological and molecular characterization of a new germplasm |
| title_sort | rescuing the brazilian agave breeding program morphophysiological and molecular characterization of a new germplasm |
| topic | Agave Biofuels genetic resources natural fibers sisal |
| url | https://www.frontiersin.org/articles/10.3389/fceng.2023.1218668/full |
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