Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected Hydrogels
Insulin loaded to the polymer network of hydrogels may affect the speed and the quality of wound healing in diabetic patients. The aim of our research was to develop a formulation of insulin that could be applied to the skin. We chose hydrogels commonly used for pharmaceutical compounding, which can...
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MDPI AG
2021-08-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/8/1215 |
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| author | Aneta Ostróżka-Cieślik Małgorzata Maciążek-Jurczyk Jadwiga Pożycka Barbara Dolińska |
| author_facet | Aneta Ostróżka-Cieślik Małgorzata Maciążek-Jurczyk Jadwiga Pożycka Barbara Dolińska |
| author_sort | Aneta Ostróżka-Cieślik |
| collection | DOAJ |
| description | Insulin loaded to the polymer network of hydrogels may affect the speed and the quality of wound healing in diabetic patients. The aim of our research was to develop a formulation of insulin that could be applied to the skin. We chose hydrogels commonly used for pharmaceutical compounding, which can provide a form of therapy available to every patient. We prepared different gel formulations using Carbopol<sup>®</sup> Ultrez<sup>TM</sup> 10, Carbopol<sup>®</sup> Ultrez<sup>TM</sup> 30, methyl cellulose, and glycerin ointment. The hormone concentration was 1 mg/g of the hydrogel. We assessed the influence of model hydrogels on the pharmaceutical availability of insulin in vitro, and we examined the rheological and the texture parameters of the prepared formulations. Based on spectroscopic methods, we evaluated the influence of model hydrogels on secondary and tertiary structures of insulin. The analysis of rheograms showed that hydrogels are typical of shear-thinning non-Newtonian thixotropic fluids. Insulin release from the formulations occurs in a prolonged manner, providing a longer duration of action of the hormone. The stability of insulin in hydrogels was confirmed. The presence of model hydrogel carriers affects the secondary and the tertiary structures of insulin. The obtained results indicate that hydrogels are promising carriers in the treatment of diabetic foot ulcers. The most effective treatment can be achieved with a methyl cellulose-based insulin preparation. |
| first_indexed | 2024-03-10T08:28:21Z |
| format | Article |
| id | doaj.art-71a378b5ede4451aac12634a38812dad |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T08:28:21Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-71a378b5ede4451aac12634a38812dad2023-11-22T09:14:26ZengMDPI AGPharmaceutics1999-49232021-08-01138121510.3390/pharmaceutics13081215Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected HydrogelsAneta Ostróżka-Cieślik0Małgorzata Maciążek-Jurczyk1Jadwiga Pożycka2Barbara Dolińska3Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Kasztanowa 3, 41-200 Sosnowiec, PolandDepartment of Physical Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, PolandDepartment of Physical Pharmacy, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, PolandDepartment of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Kasztanowa 3, 41-200 Sosnowiec, PolandInsulin loaded to the polymer network of hydrogels may affect the speed and the quality of wound healing in diabetic patients. The aim of our research was to develop a formulation of insulin that could be applied to the skin. We chose hydrogels commonly used for pharmaceutical compounding, which can provide a form of therapy available to every patient. We prepared different gel formulations using Carbopol<sup>®</sup> Ultrez<sup>TM</sup> 10, Carbopol<sup>®</sup> Ultrez<sup>TM</sup> 30, methyl cellulose, and glycerin ointment. The hormone concentration was 1 mg/g of the hydrogel. We assessed the influence of model hydrogels on the pharmaceutical availability of insulin in vitro, and we examined the rheological and the texture parameters of the prepared formulations. Based on spectroscopic methods, we evaluated the influence of model hydrogels on secondary and tertiary structures of insulin. The analysis of rheograms showed that hydrogels are typical of shear-thinning non-Newtonian thixotropic fluids. Insulin release from the formulations occurs in a prolonged manner, providing a longer duration of action of the hormone. The stability of insulin in hydrogels was confirmed. The presence of model hydrogel carriers affects the secondary and the tertiary structures of insulin. The obtained results indicate that hydrogels are promising carriers in the treatment of diabetic foot ulcers. The most effective treatment can be achieved with a methyl cellulose-based insulin preparation.https://www.mdpi.com/1999-4923/13/8/1215hydrogelinsulinin vitro drug release studyrheologytexture analysisspectroscopic methods |
| spellingShingle | Aneta Ostróżka-Cieślik Małgorzata Maciążek-Jurczyk Jadwiga Pożycka Barbara Dolińska Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected Hydrogels Pharmaceutics hydrogel insulin in vitro drug release study rheology texture analysis spectroscopic methods |
| title | Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected Hydrogels |
| title_full | Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected Hydrogels |
| title_fullStr | Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected Hydrogels |
| title_full_unstemmed | Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected Hydrogels |
| title_short | Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected Hydrogels |
| title_sort | pre formulation studies physicochemical characteristics and in vitro release kinetics of insulin from selected hydrogels |
| topic | hydrogel insulin in vitro drug release study rheology texture analysis spectroscopic methods |
| url | https://www.mdpi.com/1999-4923/13/8/1215 |
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