Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14
Persistent use of permethrin has resulted in its ubiquitous presence as a contaminant in surface streams and soils, yet little is known about the kinetics and metabolic behaviors of this pesticide. In this study, a novel bacterial strain Acinetobacter baumannii ZH-14 utilizing permethrin via partial...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00098/full |
| _version_ | 1828831926199255040 |
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| author | Hui Zhan Huishan Wang Lisheng Liao Yanmei Feng Xinghui Fan Lianhui Zhang Shaohua Chen |
| author_facet | Hui Zhan Huishan Wang Lisheng Liao Yanmei Feng Xinghui Fan Lianhui Zhang Shaohua Chen |
| author_sort | Hui Zhan |
| collection | DOAJ |
| description | Persistent use of permethrin has resulted in its ubiquitous presence as a contaminant in surface streams and soils, yet little is known about the kinetics and metabolic behaviors of this pesticide. In this study, a novel bacterial strain Acinetobacter baumannii ZH-14 utilizing permethrin via partial hydrolysis pathways was isolated from sewage sludge. Response surface methodology based on Box-Behnken design of cultural conditions was used for optimization resulting in 100% degradation of permethrin (50 mg·L−1) within 72 h. Strain ZH-14 degraded permethrin up to a concentration of 800 mg·L−1. Biodegradation kinetics analysis indicated that permethrin degradation by this strain was concentration dependent, with a maximum specific degradation rate, half-saturation constant, and inhibition constant of 0.0454 h−1, 4.7912 mg·L−1, and 367.2165 mg·L−1, respectively. High-performance liquid chromatography and gas chromatography-mass spectrometry identified 3-phenoxybenzenemethanol and 3-phenoxybenzaldehyde as the major intermediate metabolites of the permethrin degradation pathway. Bioaugmentation of permethrin-contaminated soils with strain ZH-14 significantly enhanced degradation, and over 85% of permethrin was degraded within 9 days with the degradation process following the first-order kinetic model. In addition to degradation of permethrin, strain ZH-14 was capable of degrading a large range of synthetic pyrethroids such as deltamethrin, bifenthrin, fenpropathrin, cyhalothrin, and beta-cypermethrin which are also widely used pesticides with environmental contamination problems, suggesting the promising potentials of A. baumannii ZH-14 in bioremediation of pyrethroid-contaminated terrestrial and aquatic environments. |
| first_indexed | 2024-12-12T16:43:26Z |
| format | Article |
| id | doaj.art-71abd69c8ecf4492828aeb4c906d42e0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T16:43:26Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-71abd69c8ecf4492828aeb4c906d42e02022-12-22T00:18:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-02-01910.3389/fmicb.2018.00098313623Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14Hui ZhanHuishan WangLisheng LiaoYanmei FengXinghui FanLianhui ZhangShaohua ChenPersistent use of permethrin has resulted in its ubiquitous presence as a contaminant in surface streams and soils, yet little is known about the kinetics and metabolic behaviors of this pesticide. In this study, a novel bacterial strain Acinetobacter baumannii ZH-14 utilizing permethrin via partial hydrolysis pathways was isolated from sewage sludge. Response surface methodology based on Box-Behnken design of cultural conditions was used for optimization resulting in 100% degradation of permethrin (50 mg·L−1) within 72 h. Strain ZH-14 degraded permethrin up to a concentration of 800 mg·L−1. Biodegradation kinetics analysis indicated that permethrin degradation by this strain was concentration dependent, with a maximum specific degradation rate, half-saturation constant, and inhibition constant of 0.0454 h−1, 4.7912 mg·L−1, and 367.2165 mg·L−1, respectively. High-performance liquid chromatography and gas chromatography-mass spectrometry identified 3-phenoxybenzenemethanol and 3-phenoxybenzaldehyde as the major intermediate metabolites of the permethrin degradation pathway. Bioaugmentation of permethrin-contaminated soils with strain ZH-14 significantly enhanced degradation, and over 85% of permethrin was degraded within 9 days with the degradation process following the first-order kinetic model. In addition to degradation of permethrin, strain ZH-14 was capable of degrading a large range of synthetic pyrethroids such as deltamethrin, bifenthrin, fenpropathrin, cyhalothrin, and beta-cypermethrin which are also widely used pesticides with environmental contamination problems, suggesting the promising potentials of A. baumannii ZH-14 in bioremediation of pyrethroid-contaminated terrestrial and aquatic environments.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00098/fullpermethrindegradation pathwaykineticsAcinetobacter baumanniibioremediation |
| spellingShingle | Hui Zhan Huishan Wang Lisheng Liao Yanmei Feng Xinghui Fan Lianhui Zhang Shaohua Chen Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14 Frontiers in Microbiology permethrin degradation pathway kinetics Acinetobacter baumannii bioremediation |
| title | Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14 |
| title_full | Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14 |
| title_fullStr | Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14 |
| title_full_unstemmed | Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14 |
| title_short | Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14 |
| title_sort | kinetics and novel degradation pathway of permethrin in acinetobacter baumannii zh 14 |
| topic | permethrin degradation pathway kinetics Acinetobacter baumannii bioremediation |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00098/full |
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